West China Journal of Stomatology ›› 2024, Vol. 42 ›› Issue (3): 313-318.doi: 10.7518/hxkq.2024.2023340

• Basic Research • Previous Articles     Next Articles

Correlation between FOXN3-SIN3A complex expression in peripheral blood and non-syndromic cleft lip and palate in Xinjiang

Duolikun·Wufuer (), Dilibaier·Yimingjiang , Kamilijiang·Maimaitiming , Li Jun, Wulifan·Tuoleheng   

  1. Dept. of Maxillofacial Surgery, People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830000, China
  • Received:2023-10-11 Revised:2024-04-12 Online:2024-06-01 Published:2024-05-24
  • Contact: Duolikun·Wufuer E-mail:dolkun410@aliyun.com;dolkun410@a-liyun.com
  • Supported by:
    Natural Science Foundation of Xinjiang Uygur Autonomous Region(2021D01C158)

Abstract:

Objective This work aimed to study the correlation between FOXN3-SIN3A complex expression and non-syndromic oral clefts (NSOC) in Xinjiang. Methods In this study, 60 patients with NSOC attending the People’s Hospital of Xinjiang Uygur Autonomous Region were recruited into the case group, including 30 cleft lip with or without cleft palate (NSCL/P), 30 cleft palate only (CPO), and 30 healthy children in the control group. The expression levels of FOXN3, SIN3A, and NEAT1 in peripheral blood of each group were detected by high-throughput second-generation sequencing technology and quantitative reverse transcription polymerase chain reaction (RT-qPCR). Receiver operating characteristic (ROC) curve and area under the curve (AUC) were used to analyze the diagnostic efficiency of NSOC. Results The comparison of the NSOC and control groups showed that FOXN3, SIN3A, and NEAT1 genes increased compared with the control group. The differences were all statistically significant (P<0.05). The AUCs of FOXN3, SIN3A, and NEAT1 in the NSCL/P group were 0.933 [95%CI=(0.864, 1.000)], 0.822 [(95%CI=(0.713, 0.932)], and 1.000[95%CI= (1.000, 1.000)], respectively. The AUCs of FOX-N3, SIN3A, and NEAT1 in the CPO group were 0.891 [95%CI=(0.806, 0.976)], 0.688 [95%CI=(0.552, 0.824)], and 1.000 [95%CI=(1.000, 1.000)], respectively. Conclusion The results showed a correlation between the rising gene expression of FOXN3, SIN3A, and NEAT1 in peripheral blood and the occurrence of NSOC in Xinjiang. This work provides a theoretical basis for further study of the FOXN3-SIN3A complex as biomarkers to facilitate the early screening, disease prediction, and early prevention of NSOC.

Key words: non-syndromic cleft lip and palate, FOXN3, SIN3A, NEAT1, biomarker

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