Abstract:

Objective To examine and analyze the interleukin（IL）-1B gene single nucleotide polymorphism（SNP）at positions ＋3954, and explore the association between SNP and risk of chronic periodontitis（CP） among the Hui and Dongxiang minorities in Gansu province. Methods CP group consisted of 215 patients from Hui and Dongxiang and healthy control group consisted of 219 subjects. Anti-coagulated peripheral blood sample was obtained from each subject and genomic DNA was extracted from each sample. SNP at IL-1B+3954 were analyzed by standard polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） assay. Results Statistically significant differences were found in genotype and allele frequencies between CP group and healthy control group. The exist of position alleles C increased the incidence of CP. The trend of frequency distribution of gene polymorphism above in Dongxiang was the same as that of Hui. Conclusion The present study reveals that IL-1B+3954C/T genotypes are significantly associated with the risk of CP. These results indicate that genetic polymorphism is significantly associated with the risk of CP among the Hui and Dongxiang minorities in Gansu province.

Key words: chronic periodontitis, interleukin-1, single nucleotide polymorphism