Abstract:

Objective To observe the effects of oxymatrine on the expression of interleukin-6（IL-6）, interleukin- 1β（IL-1β）mRNA of human periodontal ligament cell（PDLC） stimulated by lipopolysaccharides（LPS）, and to discuss oxymatrine’s inhibition mechanism on periodontal inflammation stimulated by LPS. Methods Firstly, isolate PDLC externally and culture them; for oxymatrine experimental group we used different combination of LPS and oxymatrine in different concentration, and for the matched group we use DEME nutrient solutions of 1% FBS. Then reverse transcription -polymerase chain reaction（RT -PCR） for checking the level of IL -6 and IL -1β mRNA. Results 25 μg·mL-1 LPS can significantly enhance the expression of both IL-6 and IL-1β mRNA’s level, and oxymatrine could restrain above phenomena. Conclusion Oxymatrine can restrain the expression of IL-6 and IL-1β mRNA of human PDLC stimulated by LPS.

Key words: oxymatrine, lipopolysaccharides, periodontal ligament cell, inflammation