关键词: 癌前病变, 腺病毒, 基因治疗

Abstract:

Objective To investigate and evaluate the appropriate virus titer and transfection efficiency of recombinant adenovirus p53 into the oral dysplastic epithelial cells（POE-9n） and provide reference for oral precancerosis research. Methods The transfection sensitivity of adenovirus into oral dysplastic epithelial cells was evaluated by the recombinant adenovirus p53 containing green fluorescent protein（rAd-GFP）. Different titre rAd-p53 was transfected into oral dysplastic epithelial cells to evaluate the effects of rAd-p53 on cell proliferation inhibition by MTT assay. The expression of exogenous p53 gene in POE-9n cells was detected by immunocytochemistry. Results More than 95% POE-9n cells were transfected by rAd-GFP with MOI from 100 to 500 and there was no statistical difference between different MOI values（r=0.124, P>0.05）. It was found that rAd-p53 had significant inhibition effects on POE- 9n cell proliferation with MOI from 100 to 500, and there were no significant differences at 96 h and 120 h after the transfection on cell proliferation inhibition（P>0.05）. P53 protein was well expressed in rAd-p53 transfected POE- 9n cells. Conclusion Exogenous p53 can be successfully transfected into POE-9n cells by rAd-p53 and the virus titer of MOI 100 was high enough to ensure efficient transfection.

Key words: pre-cancer lesion, adenovirus, gene therapy