华西口腔医学杂志

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口腔鳞状细胞癌组织中差异微小RNA/mRNA表达谱的对接研究

刘思玉1,2  谢龙1,2  祁兵3  马长艳4  桑磊1,2  李宏卫1,2   

  1. 1.南京医科大学口腔医学研究所；2.南京医科大学附属口腔医院口腔颌面外科；
    3.病理科；4.南京医科大学细胞生物学与医学遗传学系，南京 210029
  • 出版日期:2014-08-01 发布日期:2014-08-01
  • 通讯作者: 李宏卫，主任医师，博士，E-mail：lhwqhxa@sina.com
  • 作者简介:刘思玉，住院医师，硕士，E-mail：siyuliu641@163.com
  • 基金资助:

    江苏高校优势学科建设工程基金资助项目（2014-37）

Differential expression profiles of microRNAs/mRNA and docking study in oral squamous cell carcinoma

 Liu Siyu1,2, Xie Long1,2, Qi Bing3, Ma Changyan4, Sang Lei1,2, Li Hongwei1,2   

  1. 1.Institute of Stomatology, Nanjing Medical University, Nanjing 210029, China;2.Dept. of Oral and Maxillofacial Surgery, The Stomatology Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China; 3. Dept. of Oral Pathology, The Stomatology Hospital Affiliated to Nanjing Medical Uni-versity, Nanjing 210029, China; 4. Dept. of Developmental Genetics, Nanjing Medical University, Nanjing 210029, China
  • Online:2014-08-01 Published:2014-08-01

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摘要：

目的  构建分析口腔鳞状细胞癌（OSCC）中差异表达的微小RNA（miRNA）和mRNA表达谱，初步预测与OSCC发生和发展相关的 miRNA和mRNA。方法  运用高通量深度测序技术构建 miRNA和mRNA表达谱，通过 Gene Ontology功能显著性富集分析预测与OSCC细胞周期、增殖、分化和凋亡相关的miRNA和mRNA。结果  共发现77个miRNA和1 298个mRNA显著性差异表达，富集分析显示与OSCC细胞周期、增殖、分化和凋亡相关的 miRNA共73个，且一个 miRNA可调控多个 mRNA。结论  OSCC中差异表达的 miRNA和mRNA对OSCC的发生和发展有潜在的作用。

关键词: 口腔鳞状细胞癌, 微小RNA, mRNA, 高通量测序

Abstract:

 Objective  To construct and analyze the differential expression profiles of microRNAs (miRNA) and mRNA in oral squamous cell carcinoma(OSCC).   To explore the miRNA and mRNA of OSCC development and progression. Methods  The differential expression profiles of miRNA and mRNA were built by high-throughput deep sequencing technology. Using Gene Ontology (GO) enrichment analysis, the roles of differentially expressed miRNA and mRNA in cell cycle, cell proliferation, cell differentiation and cell apoptosis were analyzed. Results  Seventy-seven differentially expressed miRNA and 1 298 differentially expressed mRNAs were identified in OSCC. GO analysis showed that 73 miRNA had found target mRNA in cell cycle, cell proliferation, cell differentiation and cell apoptosis of OSCC. Moreover, a miRNA could regulate multiple mRNA. Conclusion The differential expression profiles of miRNA and mRNA have close relationship with the development and progression of OSCC.

Key words: oral squamous cell carcinoma, microRNAs, mRNA, high-throughput deep sequencing

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