关键词: 血管内皮生长因子-C, 分子克隆, DNA序列测定

Abstract:

Objective　A functional VEGF-C cDNA was cloned from a patient with squamous cell carcinoma (SCC) of tongue in orderto study the important role of vascular endothelial growth factor (VEGF)-C in lymphatic dissemination of malignan- cies in gene level.Methods　RT-PCR was employed to clone the human VEGF-C encoded cDNA from a surgical specimen of a lingual SCC patient. Then itwas subcloned into plasmid vector pCRII and sequenced.Results　A1·1 kb human VEGF-C cDNA fragmentwas amplified from the lingual SCC. The sequencing results of the fragment demonstrated that it had 99·6% similarity with the reported human VEGF-C cDNA (representing the 559~1 611 bp according the sequence of Genbank Entry X94216). Conclusion　An encoded fragment VEGF-C cDNAwas successfully cloned from a lingual SCC and provided a necessary material for further study.

Key words: vascular endothelial growth factor (VEGF)-C, molecular cloning, DNA sequencing