华西口腔医学杂志

• 专栏论著 • 上一篇    下一篇

天然药物五倍子提取物对致龋菌生长的影响

谢倩,李继遥,左渝陵,周学东   

  1. 口腔生物医学工程教育部重点实验室,四川大学,四川 成都610041
  • 收稿日期:2005-02-25 修回日期:2005-02-25 出版日期:2005-02-20 发布日期:2005-02-20
  • 通讯作者: 周学东,Tel: 028-85501439
  • 作者简介:谢 倩(1977-),女,四川人,博士
  • 基金资助:
    国家自然科学基金资助项目(30430800)

The Effect of Galla Chinensis on the Growth of Cariogenic Bacteriain Vitro

XIE Qian,LIJi-yao,ZUO Yu-lin,ZHOU Xue-dong   

  1. Key.Laboratory of Oral Biomedical Engineering ofMinistry ofEducation,Sichuan University,Chengdu610041, China
  • Received:2005-02-25 Revised:2005-02-25 Online:2005-02-20 Published:2005-02-20

摘要:

目的 研究五倍子中分离得到的不同组分对致龋菌生长的影响,以期寻找五倍子防龋的有效成分。方法 通过系统溶剂法对五倍子总鞣质分离得到4个组分,采用液体稀释法研究五倍子不同组分对6种主要致龋菌(变形链球菌,血液链球菌,唾液链球菌,内氏放线菌,粘性放线菌,乳酸杆菌)生长的影响,并使用活菌计数法测量五倍子总鞣质及1和3组分对变形链球菌生长的影响。结果 五倍子总鞣质对细菌生长的抑制作用最强;五倍子分离物中2,4组分对实验菌的抑制作用较分离物中1,3组分强,与总鞣质相当;五倍子1和3组分对变形链球菌生长均有较好的抑制作用,作用1 h后效果更明显。结论 五倍子各组分对实验菌都有一定的抑制作用,五倍子对变形链球菌的抑制作用可能通过抑制细菌代谢所需的酶类而产生,而对细菌没有直接杀灭作用。

关键词: 五倍子, 龋病, 变形链球菌

Abstract:

Objective The purples of this studywas to investigate the role of different components of Galla Chinensis extract on the growth of 6 kinds of cariogenic bacteria, and to find out the most effective components of Galla Chinensis extract.Methods  Four different components (GCE1, GCE2, GCE3 and GCE4) were separated fromGalla Chinensis and there antibacterial activities toStreptococcus mutansATCC 25175,Streptococcus sanguisATCC 10556,Streptococcus salivariusSS 196,Actinomyces naeslundii WVU627,Actinomyces viscosusATCC 19246 andLactobacillus rhamnosusAC 413 were checked. There effects on the growth curve ofStreptococcus mutansATCC 25175 were also investigated.Results The most effective part of Galla Chinensis was found to be GCE2 and GCE4, whichwere found to be a mixture of polyphenol-rich fractions. All of the different components had an in- hibitory effect to the growth ofStreptococcus mutansATCC 25175.Conclusion All of the 4 different components of Galla Chinen- sis extract could inhibit the growth of the tested bacteria. These results suggest that the antibacterial activity of Galla Chinensis ex- tract is caused by a synergistic effect of monomeric polyphenols, which can easily bind to proteins.

Key words: Galla Chinensis, caries, Streptococcus mutans