关键词: 重组人白细胞介素-1β, 双向电泳, 蛋白质组, 牙髓细胞, 肽质量指纹技术

Abstract:

Objective To compare the proteomics change of human dental pulp cells induced by recombinant human interleukin -1β（rhIL -1β）. Methods The dental pulp cell entire protein was separated by a two -dimensional electrophoresis（2-DE） technique. The rhIL-1β induction and the normal dental pulp cell protein 2-DE atlas were established. Difference expression protein was confirmed by ImageMaster 2D Elite 5.0 software analysis. To identify differentially expressed proteins spot by matrix -assisted laser desorption/ionization time of flight mass spectrometry, and get peptide mass fingerprinting. Results Comparing the two groups of protein 2 -DE atlas, 39 protein spots were obviously different. Including 15 points in the induction of protein expression were higher, 13 new protein spots, 7 protein points expressions were lower, there were only four points in the control group. After mass spectra identification, 10 protein spots were confirmed at last. Conclusion Pulp cells to rhIL-1β responsiveness is a very complex process, which involve a variety of protein molecules. rhIL-1β related 10 protein spots have been identified in the dental pulp cell for the first time. To explore pulpitis′s early response mechanism provides a new clue and ideas.

Key words: recombinant human interleukin-1β, two-dimensional electrophoresis, proteome, dental pulp cells, peptide mass fingerprint