华西口腔医学杂志

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不同脱钙条件对透明牙体标本制作影响的实验研究

李颖 周梦宇 牛卫东   

  1. 大连医科大学口腔医学院口腔内科教研室, 辽宁大连116044
  • 收稿日期:2009-02-25 修回日期:2009-02-25 出版日期:2009-02-20 发布日期:2009-02-20
  • 通讯作者: 牛卫东,Tel:0411-86110399
  • 作者简介:李颖(1981-),女,辽宁人,硕士

Effect of different decalcification condition on tooth-clearing technique

LI Ying, ZHOU Meng-yu, NIU Weidong   

  1. Dept. of Oral Medicine, School of Stomatology, Dalian Medical University, Dalian 116044, China
  • Received:2009-02-25 Revised:2009-02-25 Online:2009-02-20 Published:2009-02-20
  • Contact: NIU Weidong,Tel:0411-86110399

摘要:

目的研究硝酸的温度与浓度及附加振荡对透明牙体标本制作的影响。方法将48颗离体牙进行常规根管预备、根管充填后,根据硝酸的温度和浓度不同及是否附加振荡,随机分为6组:A组置于20 ℃、6%硝酸中;B组置于20 ℃、6%硝酸中并附加振荡;C组置于20 ℃、3%硝酸中;D组置于20 ℃、3%硝酸中并附加振荡;E组置于30 ℃、6%硝酸中并附加振荡;F组置于30 ℃、3%硝酸中并附加振荡。待标本达脱钙标准后,常规梯度脱水,于水杨酸甲酯中透明并保存,比较各组脱钙所需时间,观测牙体标本透明前后的长度,计算牙体标本透明后收缩量和收缩率。结果E组脱钙时间最快,其次是F组、B组,C组最慢。关于牙体标本收缩量,E组>B组>A组,F组>D组>C组,B组>D组,E组>D组,其差异有统计学意义(P<0.05)。C组收缩最小,与其他组之间差异有统计学意义(P<0.05)。关于牙体标本收缩率,A组与B组、C组与D组、B组与F组、D组与F组差异无统计学意义,其余各组之间比较均有统计学差异(P<0.05)。结论使用振荡仪、温度越高、酸浓度越大,脱钙越迅速,牙体标本收缩越大。使用3%浓度硝酸溶液在20 ℃振荡条件下进行脱钙,既可以尽快完成脱钙,又可以减少牙体标本的收缩程度,制作透明牙标本效果最佳。

关键词: 脱钙, 牙体收缩, 硝酸

Abstract:

Objective To evaluate shrinkage range of cleared teeth caused by nitric acid with different temperature and concentration. Methods 48 human teeth were root canal-prepared and filled, then randomly and averagely divided into six groups on the basis of temperature and density of nitric acid and the condition of whether or not added the oscillate. Group A was 20 ℃ with 6% nitric acid, group B was 20 ℃ with 6% nitric acid and oscillate, group C was 20 ℃ with 3% nitric acid, group D was 20 ℃ with 3% nitric acid and oscillate, group E was 30 ℃ with 6% nitric acid and oscillate, group F was 30 ℃ with 3% nitric acid and oscillate. After achieving the standard of the decalcification, all the specimens were gradually dehydrated, and then cleared and conserved using methyl salicylate. Time-consumed and shrinkage range of all the specimens were recorded and analyzed. Results The time of decalcification in group E was the fastest, then was group F, group B. Group C was the last one. The anastole of the specimens was group E>group B>group A, group F>group D>group C, group B>group D, group E>group D, there was significant difference(P<0.05). Group C had significant difference with other groups(P<0.05). The anastole rate of the specimens had no significant difference between group A and group B, group C and group D, group B and group F, group D and group F. Conclusion In 20 ℃, 3% nitric acid with oscillate to carry out the decalcification can use less time and get less anastole. The result of the tooth-clearing technique is the best.

Key words: decalcification, shrinkage range, nitric acid