口腔扁平苔藓基因表达谱中差异表达基因初步探讨

华西口腔医学杂志

口腔扁平苔藓基因表达谱中差异表达基因初步探讨

范媛1, 詹瑧2, 刘杰3

1.南京医科大学口腔医学研究所; 2.南京中医药大学基础医学院免疫教研室; 3.南京医科大学附属口腔医院口腔颌面外科, 江苏南京210029

收稿日期:2007-08-25 修回日期:2007-08-25 出版日期:2007-08-20 发布日期:2007-08-20
通讯作者: 范媛，Tel：025- 85031825
作者简介:范媛（1965-），女，福建人，主任医师，博士
基金资助:
国家自然科学基金资助项目（30472123）；江苏省教育厅基金资助项目（04KJD320085）

Detection of differ entially expr essed genes in oral lichen planus

FAN Yuan1, ZHAN Zhen2, LIU Jie1

1. The Research Institute of Stomatology, Nanjing Medical University, Nanjing 210029, China; 2. Dept. of Immunology, Basic Medical College of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China; 3. Dept. of Oral and Maxillofacial, Affiliated Stomatological Hospital, Nanjing Medical University, Nanjing 210029, China

Received:2007-08-25 Revised:2007-08-25 Online:2007-08-20 Published:2007-08-20
Contact: FAN Yuan，Tel：025- 85031825

摘要/Abstract

摘要：

目的筛选口腔扁平苔藓组织中的差异表达基因，并对其进行功能分类。方法用4 000种人类基因多聚酶链反应产物制成BiostarH- 40s型表达谱芯片，分离纯化正常口腔黏膜组织和口腔扁平苔藓病变组织mRNA，制备表达谱探针，用ScanArray 4000 荧光扫描仪扫描芯片荧光信号图像，分析正常口腔黏膜组织和口腔扁平苔藓组织之间差异表达的基因。结果1）在4 000条基因中，有213条基因表达差异，其中122条基因表达上调，91条基因表达下调。2）在表达上调基因中，功能分类主要包括免疫相关基因、代谢相关基因、癌基因、细胞因子、细胞信号和传递蛋白。3）在表达下调基因中，功能分类主要包括DNA结合、转录和转录因子、细胞信号和传递蛋白、免疫相关基因、细胞因子、代谢相关基因。结论口腔扁平苔藓的发生、发展过程中存在着多条不同功能基因表达调控的改变。

关键词: 口腔扁平苔藓, 基因表达谱, 基因芯片

Abstract:

Objective To detect the differentially expressed genes in oral lichen planus（OLP） by cDNA microarray. Methods The PCR products of 4 000 genes were spotted on chemical material coated glass plates in array. The total RNAs were isolated from OLP and normal oral mucosa tissue and were purified to mRNA. Both the mRNA were reversely transcribed to cDNA with the incorporations of fluorescent dUTP, for preparing the hybridization probes. The mixed probes were then hybridized to the cDNA microarray. Microarray was scanned for the fluorescent signals and showed the differences between the two samples. Results The expressions of 122 genes were up regulated while the expressions of 91 genes were down regulated in OLP among the 4 000 target genes. The up- regulated genes were mainly the ones of immunity related genes, metabolize related genes, oncogene, cytokine and cell signal transduction protein. The down- regulated genes were mainly the ones of DNA binding and transcription factors, cell signal transduction protein, immunity related genes, metabolize related genes, and cytokine. Conclusion Two hundred and thirteen differentially expressed genes with different functions were revealed in OLP, which may play some roles in the progression of OLP.

Key words: oral lichen planus, gene expression profile, gene array

范媛詹瑧刘杰. 口腔扁平苔藓基因表达谱中差异表达基因初步探讨[J]. 华西口腔医学杂志.

FAN Yuan1, ZHAN Zhen2, LIU Jie1. Detection of differ entially expr essed genes in oral lichen planus[J]. West China Journal of Stomatology.

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