关键词: 人端粒酶逆转录酶启动子, 腺病毒, 涎腺肿瘤

Abstract:

Objective To investigate the targeting expression of TRAIL gene driven by human telomerase reverse transcriptase（hTERT） promoter in SACC- 83 cell with telomerase activity. Methods Adenovirus vector AdTERTTRAIL was constructed by homologus recombination. After transfecting AdTERT- TRAIL into SACC- 83 cell and HEL cell, its effect on these cells in vitro was investigated using RT- PCR technique, MTT method and flow cytometry. Results After transfection of AdTERT- TRAIL, expression of extrinsic TRAIL gene driven was detected in SACC- 83, the proliferation of SACC- 83 cell showed significant inhibitory effect（the relative cell viability was 49.70%） and its apoptotic rate was promoted（30.49%）, whereas no TRAIL gene was detected in HEL cell, also no inhibitory effect was observed in HEL cell and its apoptotic rate showed little change. Conclusion Adenovirus vector AdTERTTRAIL was successfully constructed, which can be used to induce expression of TRAIL gene in SACC- 83 cell with targeting effect.

Key words: human telomerase reverse transcriptase promoter, adenovirus, salivary tumor