华西口腔医学杂志

• 基础研究 • 上一篇    下一篇

调宁蛋白和P63蛋白在人颌下腺中的表达

卢玉鹤;高岩   

  1. 北京大学口腔医学院口腔病理研究室, 北京100081
  • 收稿日期:2007-02-25 修回日期:2007-02-25 出版日期:2007-02-20 发布日期:2007-02-20
  • 通讯作者: 高岩,Tel:010- 62179977- 2214
  • 作者简介:卢玉鹤(1977-),女,北京人,博士研究生

Expr ession of Calponin and P63 in Human Submandibular Glands

LU Yu - he, GAO Yan   

  1. Dept. of Oral Pathology, Peking University School of Stomatology, Beijing 100081, China
  • Received:2007-02-25 Revised:2007-02-25 Online:2007-02-20 Published:2007-02-20
  • Contact: GAO Yan,Tel:010- 62179977- 2214

摘要:

目的观察新的肌上皮细胞标记物调宁蛋白和P63蛋白在人颌下腺中的表达。方法选取14个人颌下腺标本,常规福尔马林固定、石蜡包埋。用SP法进行调宁蛋白和P63蛋白的免疫组织化学染色。结果调宁蛋白在所有腺泡、闰管的外围表达,呈线形或点状包绕腺泡或导管。分泌管中也可见调宁蛋白阳性染色,主要位于近闰管、管径较小的部分。除线状、点状阳性区外,还可见锥形或三角形阳性区位于分泌管导管细胞间。P63蛋白主要表达在排泄管的外层细胞。分泌管细胞的阳性染色较弱。结论调宁蛋白可较好地标记人颌下腺肌上皮细胞;P63蛋白主要标记颌下腺排泄管的基底细胞。

关键词: 调宁蛋白, P63蛋白, 肌上皮细胞

Abstract:

Objective To observe the expression of new myoepithelial cell markers calponin and P63 in human submandibular glands. Methods Calponin and P63 antigen in routinely processed human submandibular gland tissues were immunohistochemically demonstrated by monoclonal antibodies to calponin and P63. Results Calponin expressed around all acinus and intercalated ducts as linear or punctuate pattern. Positive staining was also noted in peripheral area of some thin striated ducts that connect to intercalated ducts. Subulate or trigonal calponin expression was sometimes seen between the duct dells of striated ducts. P63 expressed mainly in the nucleus of the basal cells of excretory duct. Conclusion Calponin is an ideal marker for myoepithelial cells of human submandibular gland. P63 labels mainly the basal cells of excretory duct.

Key words: calponin, P63, myoepithelial cell