变形链球菌在日托儿童口腔中水平传播的初探

华西口腔医学杂志

变形链球菌在日托儿童口腔中水平传播的初探

尚冉1，邹静2，周学东3

1.口腔生物医学工程教育部重点实验室，四川大学；2.四川大学华西口腔医院儿童口腔科；3.牙体牙髓病科，四川成都 610041

收稿日期:2006-12-25 修回日期:2006-12-25 出版日期:2006-12-20 发布日期:2006-12-20
通讯作者: 邹静，Tel: 028-85503527
作者简介:尚冉（1980-），女，山西人，住院医师，硕士

tudy on the Horizontal Transmission of Oral Streptococcus mutans in Day-nursery Children

SHANG Ran1, ZOU Jing2, ZHOU Xue-dong3

1. Key. Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China；2. Dept. of Pediatric Dentistry, West China College of Stomatology, Sichuan University, Chengdu 610041, China；3. Dept. of Endodontics, West China College of Stomatology, Sichuan University, Chengdu 610041, China

Received:2006-12-25 Revised:2006-12-25 Online:2006-12-20 Published:2006-12-20

摘要/Abstract

摘要：

目的通过对日托儿童口腔中变形链球菌（S.mutans）水平传播的研究，为儿童龋的早期预防提供新思路。方法选择32名3～4岁日托儿童，根据是否患龋分为有龋组和无龋组。用无菌牙签采集牙面菌斑，MSB琼脂培养，微量生化反应鉴定分离S.mutans菌落，纯培养后提取基因组DNA，用AP-PCR检测其基因多态性，对来自不同个体的具有相似AP-PCR扩增图谱的菌株再进行DNA指纹分析。结果 32名受检儿童中，变形链球菌的检出率为78.1%；有龋组和无龋组的变形链球菌检出率分别为100%和69.6%。在检出变形链球菌的25名儿童中，携带有2种及以上基因型的儿童占88%；有龋组为88.9%，无龋组为87.5%，OR值为1.143，二者之间的差异无统计学意义。DNA指纹分析表明，有2种基因型变形链球菌分别在2名儿童口腔中重复检出。结论变形链球菌在日托儿童中可能存在水平传播，但其基因型检出数目与龋的发生关系不明显。

关键词: 变形链球菌, 基因型, DNA指纹分析

Abstract:

Objective To analyze genotypic diversity of oral Streptococcus mutans（S.mutans） and find out horizon-tal transmission possibility of the microbe in day-nursery children. Methods The plaque samples were scratched with sterilized toothpicks from teeth of 32 day-nursery children aged between 3 and 4, then cultured on MSB plates. Clones with representative S.mutans-like were subcultured and identified to species level biochemically. AP-PCR fingerprinting was performed to distinguish genotypic diversity of those isolates. Then S.mutans isolated from different children with very similar amplicon profiles were examined by chromosomal DNA fingerprinting analysis. Results S.mutans were isolated in oral cavities of 78.1% children, 100% in caries and 69.6% in caries-free children. A total of 57 genotypes were identified by AP-PCR. More than one amplitypes were identified in 88% of the 25 children with S.mutans colonization. Two pair of children shared common genotypic S.mutans. Conclusion There is no evident relation between number of genotype detected and caries. The presence of matching genotypes of
S. mutans among day-nursery children suggests the horizontal transmission may exist.

Key words: Streptococcusmutans, genotype, DNAfingerprinting

尚冉;邹静;周学东. 变形链球菌在日托儿童口腔中水平传播的初探[J]. 华西口腔医学杂志.

SHANG Ran1, ZOU Jing2, ZHOU Xue-dong3. tudy on the Horizontal Transmission of Oral Streptococcus mutans in Day-nursery Children[J]. West China Journal of Stomatology.

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