体外培养观察人牙囊细胞中细胞程序性死亡及其在不同流体静压力下的变化

华西口腔医学杂志

体外培养观察人牙囊细胞中细胞程序性死亡及其在不同流体静压力下的变化

金作林1，罗颂椒2，林珠1，焦光海3，王海雪1

1.第四军医大学口腔医院正畸科，陕西西安 710032；2.四川大学华西口腔医院正畸科，四川成都 610041；3.中国人民解放军总参谋部测绘技术总站，陕西西安 710054

收稿日期:2006-10-25 修回日期:2006-10-25 出版日期:2006-10-20 发布日期:2006-10-20
通讯作者: 金作林，Tel：029-84776136
作者简介:金作林（1969-），男，辽宁人，副教授，博士
基金资助:
国家自然科学基金资助项目（30400510）

Effects of Programmed Cell Death on Human Dental Follicle Cells and Changes of Programmed Cell Death under Different Hydrostatic Pressures

IN Zuo-lin1, LUO Song-jiao2, LIN Zhu1, JIAO Guang-hai3, WANG Hai-xue1

1. Dept. of Orthodontics, Stomatological College, The Fourth Military Medical University, Xi′an 710032, China; 2. Dept. of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu 610041, China; 3. General Staff Mapping Techno-unit of PLA, Xi′an 710054, China

Received:2006-10-25 Revised:2006-10-25 Online:2006-10-20 Published:2006-10-20

摘要/Abstract

摘要：

目的研究体外培养的人牙囊细胞的特征，以及在不同流体静压力作用下细胞程序性死亡（PCD）的改变，探讨PCD在牙齿萌出过程中的作用。方法取12岁男孩埋伏阻生牙齿的牙囊进行人牙囊细胞培养，将传代的培养细胞应用TUNEL的方法标记PCD细胞，分别在50 kPa和100 kPa流体静压力作用1 h后观察PCD变化，以不加压的人牙囊细胞作为对照。结果体外培养的人牙囊细胞呈梭形、纺锤形或多角形，具有成纤维细胞特征。在50 kPa流体静压力下人牙囊细胞PCD阳性细胞率与对照组无统计学差异（P>0.05），而100 kPa流体静压力下PCD阳性细胞率较对照组增加了31.65％，有统计学差异（P<0.01）。结论流体静压力可以影响体外培养的人牙囊细胞的程序性死亡。

关键词: 人牙囊细胞, 细胞程序性死亡, 流体静压力

Abstract:

Objective Tooth eruption requires the presence of the dental follicle（DF） around the unerupted tooth. This study is to investigate programmed cell death on

human dental follicle cells and changes of programmed cell death under different hydrostatic pressures: 0, 50 and 100 kPa. Methods Human dental follicles from third mandibular molars were surgically removed from adolescents who need for orthodontics treatment after informed con-tent, then trypsinized and cultured. Human dental follicle cells were divided into three groups according to different hydrostatic pressures: 0, 50 and 100 kPa and their programmed cell death were labeled by using TdT-medi-ated-dUTP nick and labeling（TUNEL）. Results Dental follicle cells cultured were elongate shape and exhibited fibroblastic
characteristics. Compared with 0 kPa, programmed cell death cells on human dental follicle cells were increased 0.23% and 31.65% under 50 kPa and 100 kPa

hydrostatic pressures respectively. 100 kPa group increased signnificantly （P<0.01）. Conclusion It suggested that programmed cell death occured in human dental follicle cells cultured in vitro and was influenced by different hydrostatic pressures. Hydrostatic pressure may improve tooth erup-tion through dental follicle.

Key words: human dental follicle cells, programmed cell death, hydrostatic pressure

金作林;罗颂椒;林珠;焦光海;王海雪. 体外培养观察人牙囊细胞中细胞程序性死亡及其在不同流体静压力下的变化[J]. 华西口腔医学杂志.

IN Zuo-lin1, LUO Song-jiao2, LIN Zhu1, JIAO Guang-hai3, WANG Hai-xue1. Effects of Programmed Cell Death on Human Dental Follicle Cells and Changes of Programmed Cell Death under Different Hydrostatic Pressures[J]. West China Journal of Stomatology.