Bcl-2与HER-2反义寡核苷酸联合转染对人舌鳞癌Tca8113细胞的干预作用

华西口腔医学杂志

Bcl-2与HER-2反义寡核苷酸联合转染对人舌鳞癌Tca8113细胞的干预作用

季平1;吴永忠2;张福军2;李少林3

1.重庆医科大学附属口腔医院颌面外科; 2. 重庆医科大学附一院颌面外科; 3.重庆医科大学核医学教研室, 重庆 400016

收稿日期:2006-04-25 修回日期:2006-04-25 出版日期:2006-04-20 发布日期:2006-04-20
通讯作者: 季平，Tel：13908302957
作者简介:季平（1962－），男，四川人，副教授，博士
基金资助:
国家自然科学基金资助项目（30070230）；重庆市科委自然科学基金资助项目（CSTC，2005DB5241）；重庆市卫生局科研项目
（052153）

Effect on Apoptosis of Tca8113 of Interference of Bcl-2 and HER-2 Genes Antisense Oligodeoxynucleotides Transfection

Jl Ping1, WU Yong-zhong2, ZHANG Fu-jun2, Li Shao-lin3

1. Dept. of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China; 2.Depts. of Oral and Maxillofacical Surgery, The First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China; 3. Dept. of Nuclear Medicine, Chongqing University of Medical Sciences, Chongqing 400016, China

Received:2006-04-25 Revised:2006-04-25 Online:2006-04-20 Published:2006-04-20

摘要/Abstract

摘要：

目的探讨Bcl-2与HER-2基因反义寡核苷酸(ASODN)联合转染对人舌鳞癌Tca8113细胞增殖的干预作用及其分子机制。方法实验分成正常对照组、Bcl-2正义干预组、HER-2正义干预组、Bcl-2反义干预组、HER-2 反义干预组、Bcl-2与HER-2基因反义寡核苷酸联合干预组,共6组。脂质体转染后不同时间分别进行电镜观察、 RT-PCR检测,以观察不同条件处理后各组细胞的凋亡、Bcl-2与HER-2基因表达有无差别。结果 Bcl-2与HER-2 基因反义寡核苷酸联合干预组,转染后出现凋亡小体和各期凋亡细胞;Bcl-2基因表达下降36%,HER-2基因表达下降51%。联合转染作用优于单反义基因转染作用。结论 Bcl-2与HER-2基因反义寡核苷酸联合转染能够促进肿瘤细胞凋亡并下调Bcl-2与HER-2基因的表达。

Abstract:

Objective To investigate the interference and the mechanisms of Bcl-2 and HER-2 genes antisense oligodeoxynucleotides combined transfection in the human tongue carcinoma Tca8113 cell lines. Methods There were 6 groups in our study, normal control group, Bcl-2 sense experimental group, HER -2 sense experimental group, Bcl-2 antisense experimental group, HER-2 antisense experimental group, Bcl-2 and HER-2 genes antisense oligodωxynucleotides combined transfection experimental group. ln the different times after liposome-mediated transfection, the cell apoptosis, Bcl-2 and HER-2 exressing level were observed by RT-PCR and electronic microscope. ResuIts According to the results of combined transfection experimental group, the apoptosis body and apoptosis cells were observed. The expression of genes were decreased statistically in both Bcl-2 and HER-2, respectively. Bcl-2 and HER-2 combined ASODN was superior to single ASODN in the intervention of tongue carcinoma. Conclusion Bcl-2 and HER-2 ASODN can effectively interfere the expression of HER-2 and Bcl-2 genes. The expression of HER-2 and Bcl-2 can be reduced, and the apoptosis of cells can be enhanced.

Key words: Tca8113celllines, HER-2andBcl-2genes, antisenseoligodeoxynucleotides, combinedtransfection

季平;吴永忠;张福军;李少林. Bcl-2与HER-2反义寡核苷酸联合转染对人舌鳞癌Tca8113细胞的干预作用[J]. 华西口腔医学杂志.

Jl Ping1, WU Yong-zhong2, ZHANG Fu-jun2, Li Shao-lin3. Effect on Apoptosis of Tca8113 of Interference of Bcl-2 and HER-2 Genes Antisense Oligodeoxynucleotides Transfection[J]. West China Journal of Stomatology.