关键词: 口腔假丝酵母菌, 药物敏感性试验, 抗真菌中药

Abstract:

Objective To evaluate the sensitivity and resistance of pathogenic oral Candida spp. isolated from head and neck cancer patients treated with radiotherapy or chemotherapy to antifungal agents. To screen antifugal agents from Chinese traditional and herbal drugs by NCCLS M27-A2 method. Methods Using YBC Test Kit to identify 20 clinical oral Candida isolated from head and neck cancer patients treated with radiotherapy or chemotherapy. The in vitro susceptibilities of 20 oral Candida spp. to 5-flucytosine（5-FC）, itraconazole（ITR）, flu- conazole（FLU）, the extracts of 6 Chinese traditional and herbal drugs（caltrop, honeysuckle flower, dandelion, green tea, pine bark, red trefoil） and utility componets of 7 Chinese traditional and herbal drugs（sophorcarpidine, aloperine, archin, glycyrrhizic acid, glycosides of white peony root, glycosides of baikal skullcap root, hydrochloric berberine）were determined by NCCLS M27-A2 method. Results The proportion of no-C.albicans in all Candida spp. were 25％. All strains were sensitive to 5-flucytosine, 25% stains were resistant to fluconazole and 40% stains were resistant to itraconazole. In all agents from Chinese traditional and herbal drugs, glycosides of white peony root and hydrochloric berberine（C20H18CINO4） exhibited antifungal activity, especially to C. glabrates. Conclusion The proportion of no-C.albicans in all oral Candida spp. isolated from head and neck cancer patients treated with radiotherapy or chemotherapy was pretty high. NCCLS M27-A2 micro-dilution method is a reliable and reproducible method and can be used to screen antifugal agents from Chinese traditional and herbal drugs.

Key words: oralCandida, antifugalsusceptibilitytesting, antifugaltraditionalChinesemedicines