关键词: 基因治疗, nm23, 阳离子脂质体, 腺样囊性癌

Abstract:

Objective　To investigate the feasibility of plasmid nm23-h1 transfection on high metastatic potential adnoid cystic carcinoma(ACC-M) cell line mediated by cationic lipid.Methods　ACC-M cell were implanted in the maxillofacial region in each of 40 BALB/c nude mice. After the tumor growth to 1 cm in diameter,0.1 ml Lipofctamine-nm23-h1 plasmid complexwere injected intratumorally in 10 mice, 3 days after the first injection,10 mices injected for twice, 10 mice as plamid-blank control, another 10 mice were injected 0.2 ml complex, 2,3,7days after the injection, the mice were killed and the specimen forHE and immunohistological chemistry study.Results　nm23-h1 expression initiated in the tumor cells 3 days after the complex injection, 7 days later, the expression level increased accompanying with extracellular matrix increase, twice injection and multiple channel injection would gain better nm23-h1 expression than once injection and single-channel injection respectively.Conclusion　Cation- ic lipid mediated nm23-h1 plamid transfecting adnoid cystic carcinoma can gain small range positive expression, but the results give little prospect for further clinical treatment in such a manner.

Key words: gene therapy, nm23, cationic lipid, adnoid cystic carcinoma