骨折愈合中纤维粘连蛋白和整合素β1的基因表达

华西口腔医学杂志

骨折愈合中纤维粘连蛋白和整合素β1的基因表达

刘少华1,程　刚2,李声伟3,田卫东3,刘　磊3

1.山东大学齐鲁医院　口腔外科,山东　济南250012;2.浙江省人民医院　口腔外科,浙江　杭州310014; 3.四川大学华西口腔医学院　口腔颌面外科学教研室,四川　成都610041

收稿日期:2005-10-25 修回日期:2005-10-25 出版日期:2005-10-20 发布日期:2005-10-20
通讯作者: 李声伟,Tel:028-85501456
作者简介:刘少华(1964-),男,山东人,副教授,博士
基金资助:
国家自然科学基金资助项目(39870750)

Study on mRNA Expression of Fibronectin and Integrinβ1 During Fracture Healing

LIUShao-hua1,CHENGGang2,LI Sheng-wei3,TIANWei-dong3,LIULei3

1.Dept.ofOral andMaxillofacialSurgery,QiluHospital ofShandong University,Ji- nan250012,China;2.Dept.ofOral andMaxillofacialSurgery,People′sHospital ofZhejiangProvince,Hangzhou310014,Chi- na;3.Dept.ofOral andMaxillofacial Surgery,West China College ofStomatology,Sichuan University,Chengdu610041,China

Received:2005-10-25 Revised:2005-10-25 Online:2005-10-20 Published:2005-10-20

摘要/Abstract

摘要： 目的　探讨骨折愈合过程中纤维粘连蛋白(FN)和整合素β1 (Itgβ1)基因时空表达变化规律。方法　取兔下颌骨骨折愈合各时期标本的石蜡切片,采用原位逆转录多聚酶链式反应技术,检测骨折愈合过程的不同时期FN 和Itgβ1 mRNA的时空表达。结果　①骨折愈合过程中,FN mRNA和Itgβ1 mRNA在骨组织的多种细胞中广泛表达,阳性信号出现于细胞浆和细胞核中;②整合素β1 mRNA在骨折5~7 d后表达增强,14~30 d最强,60~90 d时基本接近正常;③FNmRNA骨折后3 d出现阳性表达,7~14 d达到峰值,然后急剧下降,30 d时有微弱表达,60 d后未检测出表达。结论　骨折愈合过程中局部FNmRNA和Itgβ1 mRNA表达均增强,在时空关系上具有协同作用,提示 FN和Itgβ1对骨折愈合具有重要影响。

关键词: 整合素β1, 纤维粘连蛋白, 骨折愈合, 逆转录原位PCR

Abstract:

Objective　To investigate the spatial and temporal expression changes of fibronectin and integrinβ1 mRNA during fracture healing.Methods　Using in situ RT-PCR technique, the spatial and temporal expression pattern of both fibronectin and integrinβ1 mRNAwas detected on paraffined slices in different rabbit mandibular fracture healing phases.Results　①The mRNA of integrinβ1 and fibronectin was widely expressed in fractured bone cells. The positive stain existed in cytoplasm and nucleolus. ②After 7 days of fracture, increaseded integrinβ1 mRNA expression was observed, and it reached maximal levels approximately 14~30 days post-fracture and returned to normal levels after 60~90 days.③Fibronectin mRNA was detected on 3 days post- fracture, it reached maximal levels at 7~14 days,faint expressionwas detected at 30 days, undetectable 60 days afterwards.Con- clusion　During fracture healing, the mRNA expression of integrinβ1 and fibronectin increased locally, FN and Itgβ1 cooperate with each other,it is suggested that they have an important influence on fracture healing.

Key words: integrinβ1, fibronectin, fracture healing, in situ RT-PCR

刘少华,程刚,李声伟,田卫东,刘磊. 骨折愈合中纤维粘连蛋白和整合素β1的基因表达[J]. 华西口腔医学杂志.

LIUShao-hua1,CHENGGang2,LI Sheng-wei3,TIANWei-dong3,LIULei3. Study on mRNA Expression of Fibronectin and Integrinβ1 During Fracture Healing[J]. West China Journal of Stomatology.

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