华西口腔医学杂志

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艾滋病毒感染患者口腔黏膜疣状肿块人类乳头状瘤病毒感染的分型研究

马三成1,胡静1,赵今2,Paul Speight3   

  1. 1.四川大学华西口腔医院口腔领面外科;2.口腔内科,四川成都610041; 3.英国伦敦大学Eastman口腔疾病研究中心,London WCIX 8LD
  • 收稿日期:2004-10-25 修回日期:2004-10-25 出版日期:2004-10-20 发布日期:2004-10-20
  • 通讯作者: 胡静,Tel:028-85502334
  • 作者简介:马三成(1967-),男,新疆人,讲师,博士
  • 基金资助:
    中国留学基金委资助项目(99965025)

Typing Human Papillonta Virus( HPV) Infection in the Warts of Oral Mucasa from HIV-positive Patients

MA San- cheng1,HU Jing1,ZHAO Jin2,PAU1 Speight3   

  1. 1.Dept.of Oral and Maxillofacial Surgery, West China College of Stomatolo- gy, Sichuan University, Chengdu 610041,China; 2. Dept .of Oral Medicine,West China College of Stomatology, Sichuan Uni- 。勿.Chengdu 610041,China; 3. Eastnwn Dental Institute,University College of London,London WCIX 8LD, UK
  • Received:2004-10-25 Revised:2004-10-25 Online:2004-10-20 Published:2004-10-20

摘要:

目的探讨艾滋病毒感染患者口腔戮膜疵状肿块人类乳头状瘤病毒(1-IPV)感染及感染类型。方法应用 HPV Ll区通用引物Gp5十//G p6+和1-IPV特型引物(HPV6/II,16,18,31,33),通过聚合酶链反应(PCR),对34例艾滋病毒感染患者口腔豁膜沈状肿块人类乳头状瘤病毒(HPV) DNA进行检测。结果艾滋病毒感染患者口腔豁膜优状肿块中,HPV感染率为88.2%;亚型HPV6/II感染率为47.06%, HPV16感染率为II.76%, 1U〕V 18感染率为 2.94 % , HPV31感染率为5.88%。结论艾滋病毒感染患者口腔庆状肿块和H PV感染关系密切,而且大多数和低危型HPV6/II感染有关,少数病例和高危型HPV16,18,31感染有关;同一病例可以感染两种以上HPV亚型。

关键词: 庆状肿块, 人类乳头状瘤病毒, 聚合酶链反应

Abstract:

Objective To detect and type human papilloma virus (HPV) in the warts of oral mucosa from HIV-positive patients, and better understand the biological characters of these oral warts. Methods Polymerase chain reaction MR)~used to detect and type HPV infection by concensus HPV primers Gp5+/Gp6+ and specific HPV primers ( HPV6/II,16, 18, 31, 33) in 34 cases of oral mucosa warts from HIV-positive patients. Results The HPV infection rate was 88.2% by consensus HPV primers Gp5十/Gp6十;the HPV infection rate of HPV6/II,16, 18, 31 was respectively 47.06%,11.67 %;2.94%,and 5.88% by specific HPV primers. Conclusion Most lesions of oral warts from HIV-positive patients are associated with the infection of HPV. The low risk HPV6/11 infection is more common than the high risk HPV16, 18, 31.

Key words: warts, humanpapillomavirus, polymerasechainreaction