关键词: 体外微核法, 基因毒性, 纳米材料

Abstract:

Objective　The micronucleus test was applied to evaluate the genotoxicity of a new nanocomplex HA-PA66 root filling materialin vitro.Methods　The dulbecco′s modified eagle media(DMEM) extracts of the powder part and the mixture of the new nanomaterial were prepared separately. The V79 cellwas used as the test cell and the mitomycin C(MMC) as the positive control. The MTT assay was employed in our study to evaluate the cytotoxic effect while the number of micronucleus was used as the criteria forthe detection of genotoxocity.Results　TheMTTvalues in test groups and negative groupwere not significantly dif- ferent at different times (P>0.05). The number of micronucleus in test groups (powder group: 6.1±1.1/1 000; complex group: 5.7±0.6/1 000) was similar to the negative control(5.3±0.8/1 000,P>0.05), while theywere significantly different to the positive control(123.9±8/1 000,P<0.05).Conclusion　The newnanocomplexHA-PA66 root fillingmaterial showed no detectable cytotoxic and genotoxic effects in this study and was proved to be biocompatible.

Key words: in vitromicronucleus, genotoxicity, nanomaterial