关键词: 义齿粘附剂, 人口腔成纤维细胞, 细胞毒性, 四唑盐比色法

Abstract:

Objective　The purpose of this study was to evaluate thein vitrocytotoxicity of novel Comfort denture adhesive (Comfort-DA), whichwas developed by the authors, to human oral fibroblasts (HOFs).Methods　Asample of Comfort-DAwas prepared and extracted in culturing medium to prepare the eluate. Then the eluate was diluted by culturing medium to 50% and 75% concentration for the assessment of cytotoxicity by tetrazolium bromide (MTT) colorimetric assay. Wells containing freshme- dium alone were served as control. Cell viability was recorded by optical density after culturing in an atmosphere of 5% CO2and 95% air at 37℃for2, 3 and 4 days, respectively. The viability ofHOF cellswas evaluated byMTTassayto investigate cell pro- liferation. Optical density (OD) was measured by a spectrophotometer at 490 nm. Then evaluating the cytotoxicity grade in test groups according to the means of cell proliferation. ANOVAwas used to test the statistical significance.Results　The statistical analysis of the results ofMTT cytological assay indicated significant difference (P<0·05) in OD (indicate cell viability) between all concentrations of Comfort-DA and the control at all incubation times. The results of cell proliferation percentage also showed that the cytotoxicity grade of tested material only displayed“0~2”.Conclusion　The generally favorablein vitrocytotoxicity of the Comfort-DA formulations indicates that this product may be an efficacious denture adhesive.

Key words: denture adhesive, human oral fibroblast cell, cytotoxicity, tetrazolium bromide colorimetric assay