黄芩对牙髓卟啉单胞菌产丁酸影响的实验研究

华西口腔医学杂志

黄芩对牙髓卟啉单胞菌产丁酸影响的实验研究

李继遥,汤亚玲,谭红,周学东,张萍

四川大学口腔生物医学工程教育部重点实验室,四川　成都610041

收稿日期:2004-02-25 修回日期:2004-02-25 出版日期:2004-02-20 发布日期:2004-02-20
通讯作者: 谭　红,Tel:028-85501439
作者简介:李继遥(1965-),女,四川人,副教授,硕士

The Effect of Redix Scutellariae on Butyrate ofPorphyromonas endodontalis in vitro

LI Ji-yao,TANG Ya-ling,TAN Hong,ZHOUXue-dong,ZHANG Ping

KeyLab.ofOral Biomedical Engineering,Ministry ofEducation,Sichuan University, Chengdu610041,China

Received:2004-02-25 Revised:2004-02-25 Online:2004-02-20 Published:2004-02-20

摘要/Abstract

摘要：

目的　通过研究黄芩对牙髓卟啉单胞菌生长代谢的影响,探讨黄芩治疗牙髓根尖周病的作用机制。方法　采用试管两倍稀释法测定黄芩对牙髓卟啉单胞菌(P.e)的最小抑菌浓度(MIC)。采用高效液相色谱仪测定低于 MIC4个浓度的黄芩对P.e产丁酸的影响。结果　黄芩对P.e的MIC为100 mg/L。随着药物浓度升高,P.e产丁酸量降低,其值分别为(3·527±0·009) mg/L,(3·048±0·005) mg/L,(2·490±0·011) mg/L,(2·209±0·016)mg/L。结论　黄芩对P.e生长、代谢有一定的抑制作用。

关键词: 黄芩, 牙髓卟啉单胞菌, 丁酸, 高效液相色谱仪

Abstract:

Objective　To study the effect of Radix Scutellariae on the growth, metabolism ofPorphyromonas endodontalis (P.e), as a preparation for studying the mechanism of Radix Scutellariae in treating pulp and periapical diseases.Methods　 P.ewas chosen as the experimental bacteria. Radix Scutellariae was extracted by means of reflux with 80% ethanol. The value of MIC of Radix Scutellariae was measured by minute amount serial dilusion test, and the production of butyrate was measured by high liquid chromatograph(HPLC).Results　Radix Scutellariae could inhibit the growth ofP.e, of which the MICwas 100 mg/ L. Following the increase in concentration of Radix Scutellariae, the amount of butyrate decreased to (3·527±0·009) mg/L, (3·048±0·005) mg/L, (2·490±0·011) mg/L, (2·209±0·016) mg/L, respectively(P<0·05).Conclusion　Radix Scutel- lariae could inhibit the growth and metabolism ofP.eand might be an effective agent in treating pulp and periapical diseases.

Key words: Radix Scutellariae, porphyromanus endodontics, butyrate, high performance liguid chromatography

李继遥,汤亚玲,谭红,周学东,张萍. 黄芩对牙髓卟啉单胞菌产丁酸影响的实验研究[J]. 华西口腔医学杂志.

LI Ji-yao,TANG Ya-ling,TAN Hong,ZHOUXue-dong,ZHANG Ping. The Effect of Redix Scutellariae on Butyrate ofPorphyromonas endodontalis in vitro[J]. West China Journal of Stomatology.

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