基质金属蛋白酶-8在人和大鼠牙胚发育钟状期的表达

华西口腔医学杂志

基质金属蛋白酶-8在人和大鼠牙胚发育钟状期的表达

郝玉庆1,牛忠英2,王国荃3,周学东1,胡　涛1

1.四川大学教育部口腔生物医学工程教育部重点实验室,四川　成都610041; 2.北京解放军306医院　口腔科,北京100101;3.新疆医科大学公共卫生学院环境卫生系,新疆　乌鲁木齐830054

收稿日期:2004-02-25 修回日期:2004-02-25 出版日期:2004-02-20 发布日期:2004-02-20
通讯作者: 周学东,Tel:028-85402143
作者简介:郝玉庆(1965-),女,陕西人,副主任医师,博士
基金资助:
博士点基金资助项目(20030610052)

Expression of Matrixmetalloproteinase-8 on the Bell-stage in Human and Rat Tooth Development

HAO Yu-qing1,NIU Zhong-ying2,WANG Gou-quan3,ZHOUXue-dong1,HUTao1

1.KeyLab.ofOral Biomedical EngineeringMinisty ofEduca- tion,Sichuan University,Chengdu610041,China;2.Dept.of Dentistry, 306th Hospital people’s Liberation Army,Beijing 100101,China;3.Dept.ofEnvironmentHygiene,College ofPublicHygiene XinjiangMedical University,Urumchi830054,Chi- na

Received:2004-02-25 Revised:2004-02-25 Online:2004-02-20 Published:2004-02-20

摘要/Abstract

摘要：

目的　研究基质金属蛋白酶-8(MMP-8)在牙胚发育牙本质形成过程中的表达。方法　免疫组织化学方法检测MMP-8在人牙胚发育钟状期的表达;原位杂交方法检测MMP-8 mRNA在大鼠牙胚发育钟状期的表达。结果　 MMP-8在人牙胚钟状晚期硬组织形成期成牙本质细胞和牙本质基质阳性表达,近髓腔处,牙本质染色更深;MMP-8 mRNA在大鼠牙胚发育钟状早期个别分化的成牙本质细胞表达阳性,钟状晚期,即硬组织形成期,MMP-8mRNA在成牙本质细胞表达强阳性。结论　MMP-8可能参与了人和大鼠牙胚发育过程牙本质基质改建。

关键词: 基质金属蛋白酶-8, 牙胚发育, 牙本质形成

Abstract:

Objective　To study the expression of MMP-8 in human and rat tooth development.Methods　Immunohis- tochemistry was used to detect the localization of MMP-8 protein while in situ hybridization was used to examine the expression of MMP-8 mRNA.Results　The expression ofMMP-8 proteinwas localized in odontoblast and dentin matrix at the later bell stage in human tooth germ. The dentin was denser close to the pulp cavity. The expression ofMMP-8 mRNAwas found in very few polar- ized odontoblast at the early bell stage and all polarized odontoblast at the later bell stage in rat tooth germ.Conclusion　The re- sults suggested thatMMP-8 involved in dentinmatrix rebuilding in the process of dentin formation in human and rat dental develop- ment.

Key words: matrixmetalloproteinase-8, tooth development, dentin formation

郝玉庆,牛忠英,王国荃,周学东,胡涛. 基质金属蛋白酶-8在人和大鼠牙胚发育钟状期的表达[J]. 华西口腔医学杂志.

HAO Yu-qing1,NIU Zhong-ying2,WANG Gou-quan3,ZHOUXue-dong1,HUTao1. Expression of Matrixmetalloproteinase-8 on the Bell-stage in Human and Rat Tooth Development[J]. West China Journal of Stomatology.

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