成人牙周炎患者全唾液蛋白质凝胶电泳分析

华西口腔医学杂志

成人牙周炎患者全唾液蛋白质凝胶电泳分析

唐晓琳,潘亚萍,王兆元

110002　中国医科大学口腔医院口腔内科

收稿日期:2003-04-25 修回日期:2003-04-25 出版日期:2003-04-20 发布日期:2003-04-20

Sodium Dodecyle Sulphate Polyacrylamide Gel Electrophoresis of Whole Salivary Proteins from Patients with Adult Peri- odontitis

TANGXiaolin,PAN Yaping,WANG Zhaoyuan.

Oral Medicine ofSchool ofStomatology,China Medical University,Shenyang 110002,China

Received:2003-04-25 Revised:2003-04-25 Online:2003-04-20 Published:2003-04-20

摘要/Abstract

摘要：

目的　筛选与成人牙周炎(AP)相关的唾液蛋白因子。方法　分为AP组和正常对照(NC组)两组,每组25例,收集非刺激性全唾液,采用十二烷基磺酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)法分离唾液蛋白并分析其成分。AP组牙周基础治疗后2周、4周重复采样与分析。结果　AP组治疗前唾液中66 kD、18 kD、15 kD、13 kD蛋白条带容量值,51 kD、34 kD、19 kD、17 kD蛋白条带出现率都显著高于NC组;牙周基础治疗后以上各变量都显著下降。结论　18 kD、15 kD、13 kD与AP的发生与转归有关;牙周炎症状态下唾液中血清性蛋白增加。

关键词: 成人牙周炎, 唾液蛋白, 凝胶电泳分析

Abstract:

Objective　This study was intended to determine the salivary protein factors related to adult periodontitis. Methods　Twenty-five patientswith adult periodontitis (APgroup) and twenty-five normal controls (NC group, 25) were adopted in the study. Salivary protein composition in unstimulated whole saliva samples obtained before and after basic treatmentwas ana- lyzed by SDS-PAGE.Results　The volume of proteins with 66 kD, 18 kD, 15 kD, 13 kD in AP group before treatmentwere all remarkably higher than those in NC group. In addition, 51 kD, 34 kD, 19 kD, 17 kD protein bands were more frequently observed in AP group by comparison with NC group. However, after initial therapy, all the variables mentioned above decreased remarkably.Conclusion　The results demonstrated that protein bands with 18 kD, 15 kD, 13 kD might be closely related to the occurrence and recovery of periodontitis, and serum-derived proteins in saliva increased in patients with adult periodontitis.

Key words: adult periodontitis, salivary protein, gel electrophoresis analysis

唐晓琳,潘亚萍,王兆元. 成人牙周炎患者全唾液蛋白质凝胶电泳分析[J]. 华西口腔医学杂志.

TANGXiaolin,PAN Yaping,WANG Zhaoyuan.. Sodium Dodecyle Sulphate Polyacrylamide Gel Electrophoresis of Whole Salivary Proteins from Patients with Adult Peri- odontitis[J]. West China Journal of Stomatology.

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