血小板衍生生长因子BB及其受体在口腔粘膜下纤维化的超微结构定位

华西口腔医学杂志

血小板衍生生长因子BB及其受体在口腔粘膜下纤维化的超微结构定位

韩为农,彭解英,徐锡萍,彭隆祥,刘蜀凡

410078 中南大学湘雅医学院附属湘雅医院口腔科 (韩为农,彭解英,刘蜀凡),中南大学湘雅医学院电镜室(徐锡萍, 彭隆祥)

收稿日期:2002-08-25 修回日期:2002-08-25 出版日期:2002-08-20 发布日期:2002-08-20
基金资助:
本课题为湖南省科委发展基金资助项目(编号99ssy2000-14)

Ultrastructural Localization of PDGF-BB and PDGF-RBB in Oral Submucous Fibrosis

Han Weinong, Peng Jieying, Liu Shufan et al.

Han Weinong, Peng Jieying, Liu Shufan The Department ofStomatology, Xiangya Hospital, Xiangya SchoolofMedicine, CentralSouth University Xu Xiping, Peng Longxiang LaboratoryofElectronic Microscopy, Xiangya School of Medicine, CentralSouth University

Received:2002-08-25 Revised:2002-08-25 Online:2002-08-20 Published:2002-08-20

摘要/Abstract

摘要：

摘要目的:探讨血小板衍生生长因子BB(PDGF-BB)及其受体PDGF-RBB于口腔粘膜下纤维化(OSF)的关系。方法:将6例OSF组织一分为二,一部分作HE染色,作病理分期;另一部分采用包埋前染色方法,进行透射电镜观察 PDGF-BB及其受体在OSF组织中的超微结构定位。结果:OSF中PDGF-BB及其受体PDGF-RBB阳性物质主要存在于上皮细胞的线粒体、细胞膜上以及成纤维细胞、血管内皮细胞粗面内质网线粒体和细胞膜上。结论:PDGF-BB及其受体PDGF-RBB在OSF的病理生理过程中可能起重要作用。

关键词: 口腔粘膜下纤维化, 血小板衍生生长因子-BB, 血小板衍生生长因子&beta受体, 免疫电镜

Abstract:

Objective:This study aimed at investigatingwhetherPEGF-BB and PDGF-RBBwere involved in the pathogenesis of oral sub- mucous fibrosis.Methods:Immunoelectronmicroscopywas used for the study of the ultrastructural localization of PDGF-BB and re- ceptors of PDGF-RBBin 6 cases of specimens of oral submucous fibrosis. Each specimen was divided into two parts, consisting of one part for HE staining for pathological staging and the other partfor transmission electronic microscopic observation.Results:Im- munoreactive products of PDGF-BB and its receptorsmainly existed in the cellularplasm of epithelial cells, fibroblasts and vascular endothelial cells, with enlargement of acanthoid cells, swollen mitochondria and abnormal ridges. Immunoreactive products were found on the membrane of epithelial cells, aswell as in the mitochondria, rough endoplasmic reticula and on the membrane of the fibroblasts. Immunoreactive products of PDGF-RBBmainly existed in the rough endoplasmic reticula of fibroblasts and in the cell membrane of acanthoid cells, and also could be found sometimes in the rough endoplasmic reticula of vascular endothelial cells. No immunoreactive productwas found in the normal oral mucous membrane.Conclusion:The findings suggest that PDGF-BB and PDGF-RBBmight play an important role in the pathophysiological process of oral submucous fibrosis.

Key words: oral submucous fibrosis, platelet-derived growth factor-BB, receptors of platelet-derived growth factorRβ&beta, immunoelectronmicroscopy

韩为农,彭解英,徐锡萍,彭隆祥,刘蜀凡. 血小板衍生生长因子BB及其受体在口腔粘膜下纤维化的超微结构定位[J]. 华西口腔医学杂志.

Han Weinong, Peng Jieying, Liu Shufan et al.. Ultrastructural Localization of PDGF-BB and PDGF-RBB in Oral Submucous Fibrosis[J]. West China Journal of Stomatology.

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