口腔颌面部非何杰金氏淋巴瘤流式细胞DNA分析及意义探讨

华西口腔医学杂志

口腔颌面部非何杰金氏淋巴瘤流式细胞DNA分析及意义探讨

马莉,何志秀,吴兰雁,蔡益新,黄河长,雷松

510060 中山大学附属光华口腔医学院病理教研室 (马莉),四川大学华西口腔医院病理科(何志秀,吴兰雁,蔡益新),中山大学附属第三医院口腔科(黄河长),四川大学华西医院肿瘤生物治疗中心(雷松)

收稿日期:2002-06-25 修回日期:2002-06-25 出版日期:2002-06-20 发布日期:2002-06-20

Flowcytometry DNA Analysis of Oral and Maxillofacial Non-Hodgkincs Lymphoma

Ma Li, He Zhixiu, Wu Lanyan et al

Ma Li The College ofStomatology, Zhongshan University He Zhixiu, Wu Lanyan, Cai Yixin West China College of Stomatology, Sichuan University Huang Hechang The Third Affiliated Hospital of Zhongshan University Lei Song West China Hospital, Sichuan University

Received:2002-06-25 Revised:2002-06-25 Online:2002-06-20 Published:2002-06-20

摘要/Abstract

摘要：

目的:探讨流式细胞学各项指标与非何杰金氏淋巴瘤(NHL)发病部位、临床分期、病理分级、细胞类型及预后的关系和流式细胞学对NHL的诊断价值。方法:采用流式细胞仪测定50例口腔颌面部NHL及10例反应性增生淋巴结(RLN)石蜡包埋组织的DNA倍体、细胞周期各参数,并分析各参数及其与预后的关系。结果:10例RLN皆为二倍体,而NHL二倍体率为54%,异倍体率为46%,二者有显著性差异。SPF值、S+G2/M值与非何杰金氏淋巴瘤恶性程度密切相关,SPF值、S+G2/M值随肿瘤的恶性程度增大而增大。结论:口腔颌面部淋巴瘤流式细胞学检测具有一定诊断学价值,但其运用一定要与形态学相结合。同时SPF、S+G2/M等指标可以反映其细胞增殖状况及病理学恶性程度。

关键词: 流式细胞学, 非何杰金氏淋巴瘤, 反应性增生淋巴结

Abstract:

Objective:The purpose of this studywas to investigate the relationship between the results of flowcytometry analyses of differ- ent clinical stage, location, pathologic grade and cell origin of oral and maxillofacial non-Hodgkincs lymphoma (NHL), and the diagnostic value of flowcytometry analysis in lymphoma.Method:This study analyzed 50 oral and maxillofacial NHL cases and 10 reactive lymph nodes (formalin fixed and paraffin embedded) by flowcytometry (FCM).Results:Reactive lymph nodes were all diploid. The diploid rate of NHLwas 54%, and aneuploidy rate was 46%. There was statistically significant difference between reactive lymph nodes and NHLin the DNA ploidy status and cell cycle data (SPF, CV, S+G2/M, DI). The S phase fraction (SPF) and S+G2/M had close relationshipwith the grade of NHL. SPF value and DNA ploidy status had no obvious relationship with the prognosis.Conclusion:The results suggested that the FCMhad diagnostic value inNHL, especiallywhen the morphologi- cal diagnosis was difficult. Although the cell cycle data had no prognostic value, SPF and SPF+G2/M can show the proliferative status of NHL, which can help clinical doctor select therapeutic method.

Key words: flowcytometry, non-Hodgkin&prime, slymphoma, reactivelymphnodes

马莉,何志秀,吴兰雁,蔡益新,黄河长,雷松. 口腔颌面部非何杰金氏淋巴瘤流式细胞DNA分析及意义探讨[J]. 华西口腔医学杂志.

Ma Li, He Zhixiu, Wu Lanyan et al. Flowcytometry DNA Analysis of Oral and Maxillofacial Non-Hodgkincs Lymphoma[J]. West China Journal of Stomatology.