华西口腔医学杂志

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对氨基苯甲酸对乳杆菌生长影响的体外实验

郭斌,周学东,肖晓蓉,李继遥,李蕾   

  1. 610041 四川大学华西口腔医学院
  • 收稿日期:2001-10-25 修回日期:2001-10-25 出版日期:2001-10-20 发布日期:2001-10-20
  • 基金资助:
    本课题为国家自然科学基金资助项目(编号39670785)

Effects of Para-aminobenzoic Acid (PABA) on Growth of Lactobacillus acidophilus

Guo Bin, Zhou Xuedong, Xiao Xiaorong, et al   

  1. College of Stomatology,West China University of Medical Sciences
  • Received:2001-10-25 Revised:2001-10-25 Online:2001-10-20 Published:2001-10-20

摘要:

目的:探讨对氨基苯甲酸菌株(PABA)对牙本质龋、根面龋致病菌之一的乳杆菌生长的影响。方法:10倍系列稀释法配制浓度为10-3~10-10gPL的PABA液,将其分别加入改良的Carlsson培养基中,再将乳杆菌ATCC4356放入该培养基厌氧培养48 h(80%N2、10%H2、10%CO2),用紫外可见分光光度仪测定细菌浓度OD值(K=540 nm);并在琼脂平板培养观察细菌生长的情况,进行菌落计数。结果:PABA也有促进乳杆菌生长的能力,当PABA的浓度从 10-10~10-4gPL增加时,其促进乳杆菌生长的能力增强(P<0.05),且当浓度为10-5gPL时,促进乳杆菌生长的能力达最强,随着PABA的浓度增加到10-4gPL时,此促进作用开始下降,PABA的浓度再增加至10-3gPL时,对乳杆菌的生长无明显的促进作用。结论:PABA的浓度为10-5gPL时,促进乳杆菌生长的能力达最强,高于或低于此浓度,这种促生长的作用均下降。

关键词: 乳杆菌, 对氨基苯甲酸, 细菌生长, 共生

Abstract:

Objective:The aim of this study is to examine effects of para-aminobenzoic acid (PABA) on the growth ofLactobacillus aci- dophilus(L.acidophilus).Methods:Different concentrations of PABA (10-10~10-3gPL) were separately transferred to the modi- fied Carlsson medium.L.acidophilus(ATCC4356) grew in these Carlsson media. All cultureswere incubated at 37eanaerobi- cally in atmosphere of 80% of nitrogen, 10% of hydrogen, and 10% of carbon dioxide for 48 hours. Absorbance values (K= 540nm) of bacterial suspensions were measured using a spectrometer (UV-1601). Colony forming units (CFU) were obtained by growingL.acidophilusin Carlsson media with different concentration of PABA (10-10~10-3gPL).Results:Different concentra- tions of PABA (10-10~10-4gPL) had different stimulating effects on the growth ofL.acidophilus(P<0.05). But stimulating ef- fects declined, when PABA concentrationwas 10-5gPL, andwhen the concentration of PABA reached 10-3gPL, the stimulating ef- fect disappeared.Conclusion:This study indicates PABA stimulates the growth ofL.acidophilus, and PABA can promote growth ofL.acidophilus.

Key words: Lactobacillus acidophilus, para-aminobenzoic acid, growth