华西口腔医学杂志

• 基础研究 • 上一篇    下一篇

弱激光照射对兔牙移动速度及骨改建影响的实验研究

孙新华,朱宪春,徐成伟,叶耐永,朱虹   

  1. 130041 吉林大学口腔医院
  • 收稿日期:2001-10-25 修回日期:2001-10-25 出版日期:2001-10-20 发布日期:2001-10-20
  • 基金资助:
    本课题为吉林省科委资助项目(编号970322)

Effects of Low Energy Laser on Tooth Movement and Remodeling of Alveolar Bone in Rabbits

Sun Xinhua, Zhu Xianchun, Xu Chengwei, et al   

  1. School of Stomatology,Jilin University
  • Received:2001-10-25 Revised:2001-10-25 Online:2001-10-20 Published:2001-10-20

摘要:

目的:探讨弱激光照射对兔实验性牙齿移动速度及骨改建作用的影响。方法:将42只大耳白兔随机分为7 组,即正常组、实验1、3、5、7、14、21 d组。2%戊巴比妥钠麻醉下于双侧上颌第一磨牙与切牙间拴结不锈钢螺簧,施力80 g。左侧为对照侧,右侧为照射侧,进行弱激光照射。通过计算机测量分析牙齿移动的距离,HE染色进行组织学观察和破骨细胞计数。结果:经弱激光照射侧牙齿移动距离普遍大于对照侧,1、3、14、21 d有显著差异。光镜下的组织学观察显示,经弱激光照射侧血管化反应、破骨与成骨活动均较对照侧活跃。破骨细胞计数3、5、7 d组与对照侧相比有显著差异(P<0101,P<0.05)。结论:弱激光照射促进了兔实验性牙齿移动速度与骨改建。

关键词: 弱激光, 牙齿移动, 骨改建

Abstract:

Objective:The aim of this studywas to investigate effects of lowenergy laseron the experimental toothmovement and the re- modeling of alveolar bone in rabbits.Methods:A total of 42 white rabbits were chosen and randomized into one control group and six experimental groups, with 6 rabbits in each group. After anesthesia with 2% pentobaritone sodium, orthodontic appliances consisting of a coil spring connected bilaterally the upper firstmolarwith the upper incisor by using a ligature wire. The force ex- erted at the time of insertion was approximately 80 g. The left side served as the control side, and the right side was the experi- mental side treated by receiving irradiation of lowenergy laser. The treatmentperiods of differentgroups lasted separately for1, 3, 5, 7, 14, 21 days respectively. The displacement extent of teeth was measured by employing the computer image analyzing sys- tem. The resultswere analyzed statistically. ThroughHE staining, the histomorphological characterof tissue around firstmolarwas also investigated, and numbers of osteoclasts were counted.Results:The displacement extent of teeth on the experimental side, which was irradiated by low energy laser, was more obvious than that of the normal control side. The difference was statistically significant 1, 3, 14, 21 days after the beginning of the treatment. Through histological observation under a lightmicroscope, the osteoclasts and osteoblasts on the experimental side remained more active than those of the control side. There is significant differ- ence in amount of osteoclasts between the experimental and the control sides, 3,5 or 7 days after the treatment (P<0.01, P<0.05).Conclusion:The irradiation of low energy laser promote tooth movement and remodeling of alveolar bone.

Key words: low energy laser, tooth movement, the remodeling of alveolar bone