香芹酚水凝胶对牙周炎大鼠牙槽骨保护作用研究

doi:10.7518/hxkq.2024.2024037

摘要/Abstract

摘要：

目的本研究旨在探究香芹酚水凝胶对牙周炎大鼠牙槽骨的保护作用及机制。方法以泊洛沙姆、羟丙基甲基纤维素为基质，制备负载香芹酚的温敏型水凝胶。SD大鼠经结扎线法造模，随机分为空白对照组、牙周炎组、空白水凝胶组及低、中、高剂量香芹酚水凝胶组，观察各组大鼠牙周炎症状及牙槽骨CT结构；观察肝、脾、肾及牙周组织变化；检测血清中骨代谢指标；Western blot检测骨保护素（OPG）及核因子-κB（NF-κB）通路蛋白表达；定量反转录聚合酶链反应（qRT-PCR）检测炎性因子水平。结果香芹酚水凝胶具有良好缓释性、生物相容性及细胞黏附性；香芹酚水凝胶组大鼠牙周炎症状明显减轻，牙龈组织中OPG蛋白表达显著增加（P<0.01），核因子-κB受体活化因子配体（RANKL）、核因子-κB受体活化因子（RANK）、NF-κB蛋白水平及各炎症因子水平大幅降低（P<0.01）。结论香芹酚水凝胶可调节OPG、NF-κB通路，减少牙槽骨吸收，改善牙周炎症。

关键词: 牙周炎, 香芹酚, 水凝胶, 骨保护素通路, 核因子-κB通路, 牙槽骨吸收

Abstract:

Objective This study aimed to investigate the protective effect and mechanism of carvacrol hydrogel on the alveolar bone in rats with periodontitis. Methods A thermosensitive hydrogel supported by carvacrol was prepared using poloxamer and hydroxypropyl methyl cellulose as matrix. SD rats were randomly divided into blank group, periodontitis group, blank hydrogel group, and low-, medium-, and high-dose hydrogel groups. The periodontitis symptoms and the CT structure of the alveolar bone were observed. The changes in liver, spleen, kidney, and periodontal tissues were observed. The related indexes of bone metabolism in serum were detected. The expression of osteoprotegerin (OPG) and nuclear transcription factor-κB (NF-κB) pathway proteins was determined by Western blot. The levels of inflammatory factors were assessed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results Carvacrol hydrogel had good slow release, biocompatibility, and cell adhesion. The periodontitis of rats in the carvacrol hydrogel group was significantly alleviated, the expression of OPG protein in gingival tissue was significantly increased (P<0.01), and the levels of receptor activator of NF-κB ligand (RANKL), receptor activator of NF-κB (RANK), NF-κB protein, and inflammatory factors were significantly decreased (P<0.01). Conclusion Carvacrol hydrogel can regulate the OPG and NF-κB pathways, reduce alveolar bone absorption, and improve periodontal inflammation.

Key words: periodontitis, carvacrol, hydrogel, osteoprotegerin pathway, nuclear transcription factor-κB pathway, alveolar bone resorption

中图分类号:

Q257

周露露, 滕念, 高甜甜, 王洪斌, 高翔. 香芹酚水凝胶对牙周炎大鼠牙槽骨保护作用研究[J]. 华西口腔医学杂志, 2024, 42(5): 593-608.

Zhou Lulu, Teng Nian, Gao Tiantian, Wang Hongbin, Gao Xiang. Protective effect of carvacrol hydrogel on the alveolar bone in rats with periodontitis[J]. West China Journal of Stomatology, 2024, 42(5): 593-608.

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基因	上游引物（5’-3’）	下游引物（5’-3’）
OPG	CTCACTTGGCCTCCTGCTAA	TCGCACAGGGTGACATCTATT
RANKL	TTCTCAGGAGTTCCAGCTATGATG	GCCCAGCCTCGATCATGGTA
RANK	GCTCAACATCCCTTGCAGCTC	GTCCCTTGGTGTGCTTCCAT
TNF-α	GGGTGATCGGTCCCAACAAG	GCTTGGTGGTTTGCTACGA
IL-1β	GCTTCCTTGTGCAAGTGTC	TGGACAGCCCAAGTCAAGG
IL-6	GTTTCTCTCCGCAAGAGACTTC	TGTGGGTGGTATCCTCTGTG
GAPDH	AGTGCCAGCCTCGTCT	CCGTGGGTAGAGTCATACTGG

组别	SBI/分	PD/mm
C	0.13±0.05^Be	0.21±0.05^Bd
P	2.50±0.29^Aa	1.04±0.04^Aa
B	2.17±0.18^Aa	0.97±0.05^Aa
L	1.38±0.23^Ab	0.76±0.08^Ab
M	0.45±0.24^Ac	0.57±0.05^Ac
H	0.19±0.07^Bd	0.24±0.04^Bd

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