Er:YAG激光-氟化钠联合法与亚甲基蓝-光动力疗法防龋效果及安全性的比较

doi:10.7518/hxkq.2020.06.005

华西口腔医学杂志 ›› 2020, Vol. 38 ›› Issue (6): 637-641.doi: 10.7518/hxkq.2020.06.005

Er:YAG激光-氟化钠联合法与亚甲基蓝-光动力疗法防龋效果及安全性的比较

张蕾(), 于旭红, 姚丽萍

烟台市口腔医院,烟台 264008

收稿日期:2019-12-31 修回日期:2020-08-28 出版日期:2020-12-01 发布日期:2020-12-07
通讯作者: 张蕾 E-mail:zhangleibaby1984@126.com
作者简介:张蕾,主治医师,硕士,E-mail：zhangleibaby1984@126.com
基金资助:
烟台市重点研发计划(卫生项目)(2016WS055)

Comparison of the effect and safety of Er:YAG laser combined with fluoride and methylene blue-photodynamic therapy on caries prevention

Zhang Lei(), Yu Xuhong, Yao Liping

Yantai Stomatological Hospital, Yantai 264008, China

Received:2019-12-31 Revised:2020-08-28 Online:2020-12-01 Published:2020-12-07
Contact: Zhang Lei E-mail:zhangleibaby1984@126.com
Supported by:
Key Research and Development Plan (Health Projects) in Yantai City(2016WS055)

摘要/Abstract

摘要：

目的比较Er:YAG激光-氟化钠联合法与亚甲基蓝-光动力疗法（MB-PDT）的防龋效果及安全性。方法建立28只大鼠龋齿模型,随机分成7组：不同功率密度的光动力疗法（PDT）组、激光+氟化钠联合组、激光组、氟化钠组和0.9%生理盐水组。分光光度法检测变异链球菌生长抑制情况;激光荧光诊断仪（LF）检测龋损脱矿程度;组织病理观察牙髓及颊黏膜损伤状况。结果 PDT组与激光+氟化钠联合组光密度值明显低于其他处理组（P<0.05）;不同处理方法均可使LF值上升,出现不同程度的脱矿;组织病理学观察,70 mw·cm^-2的联合组和Er:YAG激光组存在牙髓及颊黏膜损伤。结论相同参数下,联合组与PDT均有良好的杀菌效果,但PDT不良反应少、损伤小,是一种高效且安全的防龋手段。

关键词: 光动力疗法, Er:YAG激光, 氟化物, 组织病理

Abstract:

Objective To compare the anti-caries effect and safety of Er:YAG laser combined with fluoride and methylene blue-photodynamic therapy (MB-PDT). Methods A total of 28 rat dental caries models were established and randomly divided into seven groups: photodynamic therapy (PDT) group, laser combined with fluoride group, laser group, sodium fluoride group, and 0.9% saline control group. Spectrophotometric optical density was used to reflect the growth of Streptococcus mutans. Laser-induced fluorescence diagnostic (LF) instrument was utilized to detect the demineralization degree of dental caries. Histopathological sections were employed to observe the damage of dental pulp and buccal mucosa. Results The optical density (OD) value of the PDT and combination groups was significantly lower than that of other treatment groups (P<0.05). An increase in LF value and demineralization occurred in varying degrees with different treatment methods. Histopathological observation showed that pulp and buccal mucosa injury was more obvious in the combination group of 70 mw·cm^-2 and Er:YAG laser group compared with other groups. Conclusion Under the same parameters, the combined group and PDT have good germicidal efficacy, but PDT has fewer adverse reactions and less damage. It is an effective and safe method for caries prevention.

Key words: photodynamic therapy, Er:YAG laser, fluoride, histopathology

中图分类号:

R781.05

张蕾, 于旭红, 姚丽萍. Er:YAG激光-氟化钠联合法与亚甲基蓝-光动力疗法防龋效果及安全性的比较[J]. 华西口腔医学杂志, 2020, 38(6): 637-641.

Zhang Lei, Yu Xuhong, Yao Liping. Comparison of the effect and safety of Er:YAG laser combined with fluoride and methylene blue-photodynamic therapy on caries prevention[J]. West China Journal of Stomatology, 2020, 38(6): 637-641.

图/表 4

参考文献 14

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组别	初始值	终末值	增加量
A组	3.56±0.67	13.47±3.16	9.91±2.46*
B组	3.85±0.56	13.36±3.75	9.51±2.77*
C组	3.71±0.73	11.18±4.91	7.47±2.79*
D组	3.88±0.66	11.19±3.21	7.31±1.96*
E组	3.76±0.62	12.25±4.02	8.49±1.88*
F组	3.73±0.58	15.51±4.19	11.78±2.21*
G组	3.79±0.61	24.19±4.31	20.42±3.12

组别	0分	1分	2分	3分
A组	32	0	0	0
B组	30	2	0	0
C组*	25	5	2	0
D组*	23	6	3	0
E组*	22	6	3	1
F组	32	0	0	0
G组	32	0	0	0

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Er:YAG激光-氟化钠联合法与亚甲基蓝-光动力疗法防龋效果及安全性的比较

Comparison of the effect and safety of Er:YAG laser combined with fluoride and methylene blue-photodynamic therapy on caries prevention

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