新型抗菌自修复自粘接树脂水门汀的制备及性能研究

doi:10.7518/hxkq.2020.03.005

华西口腔医学杂志 ›› 2020, Vol. 38 ›› Issue (3): 256-262.doi: 10.7518/hxkq.2020.03.005

新型抗菌自修复自粘接树脂水门汀的制备及性能研究

胡格¹, 张新艳¹, 赵家鑫², 周传健², 吴峻岭¹()

1.山东大学口腔医学院口腔修复学教研所,山东省口腔组织再生重点实验室,山东省口腔生物材料与组织再生工程实验室,济南 250012
2.山东大学材料科学与工程学院高分子材料研究所,济南 250061

收稿日期:2019-04-11 修回日期:2019-06-04 出版日期:2020-06-01 发布日期:2020-05-28
通讯作者: 吴峻岭 E-mail:doctorwujunling@163.com
作者简介:胡格,硕士,E-mail: hugedentist@163.com
基金资助:
国家自然科学基金面上项目(81671032)

Development of novel self-adhesive resin cement with antibacterial and self-healing properties

Hu Ge¹, Zhang Xinyan¹, Zhao Jiaxin², Zhou Chuanjian², Wu Junling¹()

1.Dept. of Prosthodontics, School of Stomatology, Shandong University, Shandong Key Laboratory of Oral Tissue Regeneration, Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan 250012, China
2.Research Institute of Polymer Materials, School of Materials Science and Engineering, Shandong University, Jinan 250061, China

Received:2019-04-11 Revised:2019-06-04 Online:2020-06-01 Published:2020-05-28
Contact: Junling Wu E-mail:doctorwujunling@163.com
Supported by:
The National Natural Science Foundation of China(81671032)

摘要/Abstract

摘要：

目的制备新型具有抗菌性能的自修复自粘接树脂水门汀,并探讨其粘接强度、力学性能、自修复效率及抗菌性能等。方法制备长链烷基季铵盐修饰的纳米二氧化硅抗菌填料,分别以0、2.5%、5.0%、7.5%、10.0%不同的质量百分比添加到自行合成的含自修复微胶囊的自粘接树脂水门汀中,生成具有抗菌性能的新型自修复自粘接树脂水门汀。用剪切试验来测试其牙本质粘接强度;用三点弯曲试验来测试其弯曲强度和弹性模量;用单刃V形切口梁法来测试其断裂韧度及自修复效率;建立人牙菌斑生物膜体外模型,通过菌落形成单位计数、乳酸代谢分析等来评价其对人牙菌斑生物膜的影响。结果当抗菌填料含量质量百分比达到7.5%时,牙本质粘接强度出现显著降低（P<0.05）;加入0、2.5%、5.0%、7.5%、10.0%不同质量百分比的纳米抗菌无机填料时,新型树脂水门汀的弯曲强度、弹性模量、断裂韧度及自修复效率均没有显著变化（P>0.1）;当抗菌填料含量质量百分比达到2.5%时,新型树脂水门汀对人牙菌斑生物膜代谢产生明显抑制作用,显示了良好的抗菌性能（P<0.05）。结论含一定量纳米抗菌无机填料的新型自修复自粘接树脂水门汀具有较强的抗菌性能及自修复功能,为进一步临床应用打下了基础。

关键词: 抗菌性能, 自修复, 微胶囊, 自粘接, 树脂水门汀

Abstract:

Objective This study aimed to develop novel self-adhesive resin cement with antibacterial and self-healing properties. Furthermore, the dentin bonding strength, mechanical properties, self-healing efficiency, and antibacterial property of the developed cement were measured. Methods Novel nano-antibacterial inorganic fillers that contain quaternary ammonium salts with long-chain alkyls were synthesized. These fillers were added into self-adhesive resin cement containing self-healing microcapsules at mass fractions of 0, 2.5%, 5.0%, 7.5%, or 10.0%. The dentin shear bonding test was used to test the bonding strength, whereas the flexural test was used to measure the flexural strength and elastic modulus of the cement. The single-edge V-notched beam method was used to measure self-healing efficiency, and human dental plaque microcosm biofilms were chosen to calculate the antibacterial property. Results The dentin shear bond strength significantly decreased when the mass fraction of the nano-antibacterial inorganic fillers in the novel cement reached 7.5% (P<0.05). The incorporation of 0, 2.5%, 5.0%, 7.5%, or 10.0% mass fraction of nano-antibacterial inorganic fillers did not adversely affect the flexural strength, elastic modulus, fracture toughness, and self-healing efficiency of the cement (P>0.1). Resin cement containing 2.5% mass fraction or more nano-antibacterial inorganic fillers significantly inhibited the metabolic activity of dental plaque microcosm biofilms, indicating strong antibacterial potency (P<0.05). Conclusion The novel self-adhesive resin cement exhibited promising antibacterial and self-healing properties, which enable the cement to be used for dental applications.

Key words: antibacterial property, self-healing, microcapsule, self-adhesive, resin cements

中图分类号:

R783.1

胡格, 张新艳, 赵家鑫, 周传健, 吴峻岭. 新型抗菌自修复自粘接树脂水门汀的制备及性能研究[J]. 华西口腔医学杂志, 2020, 38(3): 256-262.

Hu Ge, Zhang Xinyan, Zhao Jiaxin, Zhou Chuanjian, Wu Junling. Development of novel self-adhesive resin cement with antibacterial and self-healing properties[J]. West China Journal of Stomatology, 2020, 38(3): 256-262.

图/表 4

图 1

表 1

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表 3

参考文献 17

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组别	粘接强度/MPa	弯曲强度/MPa	弹性模量/GPa
0	7.8±1.0^a	108.8±16.8^a	5.7±0.9^a
2.5%	7.4±1.3^a	102.6±17.1^a	5.2±1.0^a
5.0%	7.7±1.2^a	107.9±17.1^a	5.4±1.0^a
7.5%	5.7±1.1^b	100.8±16.8^a	5.5±1.1^a
10.0%	5.3±1.0^b	104.5±16.6^a	4.9±0.8^a
F值	5.5	0.4	0.8
P值	<0.05	>0.1	>0.1

组别	原始断裂韧度/	自修复后断裂韧度/	自修复效率/%
组别	（MPa·m^1/2）	（MPa·m^1/2）	自修复效率/%
0	1.1±0.2^a	0.8±0.2^a	69.9±11.2^a
2.5%	1.0±0.2^a	0.7±0.1^a	71.9±14.0^a
5.0%	1.1±0.2^a	0.7±0.1^a	68.2±11.4^a
7.5%	1.0±0.2^a	0.8±0.1^a	73.9±12.0^a
10.0%	1.1±0.2^a	0.7±0.1^a	68.4±11.7^a
F值	0.1	0.1	0.2
P值	>0.1	>0.1	>0.1

组别	CFU			乳酸量/（mmol·L^-1）
组别	全菌落	总链球菌	变异链球菌	乳酸量/（mmol·L^-1）
0	（5.5±1.1）×10⁹*	（5.3±0.8）×10⁸*	（5.3±1.0）×10⁷*	24.10±4.76*
2.5%	（5.1±0.9）×10^6#	（5.4±0.9）×10^5#	（5.4±1.0）×10^3#	0.24±0.04^#
5.0%	（5.5±1.0）×10^6#	（5.7±1.0）×10^5#	（5.7±0.9）×10^3#	0.22±0.03^#
7.5%	（5.7±1.1）×10^6#	（5.5±1.0）×10^5#	（5.9±1.1）×10^3#	0.22±0.04^#
10.0%	（5.9±1.0）×10^6#	（5.5±1.1）×10^5#	（5.8±1.0）×10^3#	0.27±0.05^#
F值	153.9	239.7	178.4	151.0
P值	<0.05	<0.05	<0.05	<0.05

新型抗菌自修复自粘接树脂水门汀的制备及性能研究

Development of novel self-adhesive resin cement with antibacterial and self-healing properties

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