华西口腔医学杂志

• 基础研究 • 上一篇    下一篇

骨形态发生蛋白受体2在维甲酸诱导腭裂小鼠胚胎腭突中的表达

陈沐1  刘学1  余东升2  王成2  王伟财2  黄洪章2   

  1. 1.广东医学院附属南山医院口腔科,深圳 518052;
    2.中山大学光华口腔医学院•附属口腔医院口腔颌面外科,中山大学口腔医学研究所,广州 510055
  • 出版日期:2015-08-01 发布日期:2015-08-01
  • 通讯作者: 黄洪章,教授,硕士, E-mail:huanghongzhang@gmail. com
  • 作者简介:陈沐,副主任医师,博士,E-mail:chenmove@163.co
  • 基金资助:

    国家自然科学基金资助项目( 81300862);深圳市医疗卫生类科研基金资助项目(201302202);深圳市南山区卫生科技基金资助项目(2012040)

Expression of bone morphogenetic protein receptor 2 in cleft mouse embryonic palate induced by retinoic acid

Chen Mu1, Liu Xue1, Yu Dongsheng2, Wang Cheng2, Wang Weicai2, Huang Hongzhang2   

  1. 1. Dept. of Stomatology, Nanshan Affiliated Hospital of Guangdong Medical College, Shenzhen 518052, China; 2. Dept. of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Hospital of Stomatology, Institute of Stomatological Research, Sun Yat-sen University, Guangzhou 510055, China
  • Online:2015-08-01 Published:2015-08-01

摘要:

目的  探讨全反式维甲酸(atRA)对小鼠胚胎腭突中骨形态发生蛋白受体 2(BMPR2)表达的影响。方法 通过 atRA灌胃的方法建立 atRA诱导的小鼠腭裂模型,取妊娠 15 d(GD15)和 17 d的( GD17)的胚胎腭部进行苏木精-伊红染色,并用免疫组织化学及逆转录聚合酶链式反应技术检测 BMPR2在胚胎腭部的表达。结果 atRA诱导小鼠形成体积较小的畸形腭突和明显的中缝腭裂畸形。 BMPR2在GD15和GD17正常胚胎腭部有高水平的阳性表达,但在腭裂胚胎腭部的表达水平明显减弱。正常胚胎腭部 Bmpr2 mRNA在GD15和GD17的表达水平均明显高于腭裂胚胎( P<0.05)。结论 atRA可导致小鼠胚胎腭突发育不良形成腭裂,并显著下调 BMPR2表达水平,从而影响腭部发育的正常分子调控过程。

关键词: 骨形态发生蛋白受体2, 维甲酸, 腭裂

Abstract:

Objective To investigate the effects of all-trans retinoic acid (atRA) on the function of bone morphogenetic protein receptor 2 (BMPR2) expression in embryonic palate. Methods Cleft palate mice model was established by atRA. On gestation day (GD) 15 and GD17, the pregnant mice were killed to obtain the embryos from the uteri. The embryonic palates were stained with hematoxylin-eosin,and the remaining sections were used for the immunohistochemistry of BMPR2 detection. Reverse transcription-polymerase chain reaction was performed to detect the expression levels of Bmpr2 mRNA. Results In the atRA-treated group, short extensions and failure to fuse with each other were observed. The positive expression of BMPR2 was detected in developing palatal process from GD 15 to GD 17 in the control group. Compared with those of the control group, BMPR2 protein and Bmpr2 mRNA decreased in the atRA-treated group (P<0.05). Conclusion The treatment of pregnant mice with retinoic acid produces small palatal shelves in their fetuses and down-regulates BMPR2 expressions.

Key words: bone morphogenetic protein receptor 2, retinoic acid, cleft palate