华西口腔医学杂志

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纤维蛋白胶不同复合方式对牙囊细胞增殖活性的影响

刘红1,2 杨超2,3 陈国庆2,3 田卫东2,4 陈宇1   

  1. 1.口腔疾病研究国家重点实验室 华西口腔医院病理科（四川大学）；2.口腔再生医学国家地方联合工程实验室（四川大学）；3.口腔疾病研究国家重点实验室 华西口腔医院（四川大学）；4.创伤整形外科，成都 610041
  • 收稿日期:2014-03-26 修回日期:2014-12-20 出版日期:2015-04-01 发布日期:2015-04-01
  • 通讯作者: 陈宇，教授，博士，E-mail：chen-yu55@hotmail.com
  • 作者简介:刘红，硕士，E-mail：liuhongxiaodi@163.com
  • 基金资助:

    国家自然科学基金资助项目（81271095，81271119，81200792）；高等学校博士学科点专项科研基金资助项目（20110181120067，20110181110089）；四川省科技支撑计划基金资助项目（2012SZ0013，12ZC0493）；四川省自然科学基金资助项目（2011JY0125，12JC0212）

Effects of different fibrin glue combination modes on the proliferation and viability of dental follicle cells

Liu Hong1,2, Yang Chao2,3, Chen Guoqing2,3, Tian Weidong2,4, Chen Yu1.   

  1. 1. State Key Laboratory of Oral Diseases, Dept. of Pathology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; 2. National Engineering Laboratory for Oral Regenerative Medicine, Sichuan University, Chengdu 610041, China; 3. State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; 4. State Key Laboratory of Oral Diseases, Dept. of Traumatic and Plastic Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2014-03-26 Revised:2014-12-20 Online:2015-04-01 Published:2015-04-01

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摘要：

目的 研究纤维蛋白胶与人牙囊细胞（DFCs）二维（2D）及三维（3D）结合模式对细胞存活、增殖及凋亡的影响，评估纤维蛋白胶作为细胞移植载体的可行性及有效性。方法 采用细胞膜绿色荧光探针（DIO）对存活DFCs细胞膜进行标记，采用ImageJ2x软件细胞计数；采用碘化丙啶（PI）对凋亡细胞进行染色。结果 纤维蛋白胶在3D-2和2D-1复合模式下的降解速度快于3D-1模式；纤维蛋白原浓度为15 mg•mL-1辅以凝血酶浓度2 U•mL-1条件下，DFCs能够存活并生长良好，尤其在3D-1结合模式下更有利于细胞增殖及伸展。结论 纤维蛋白胶可作为一种有效的细胞移植载体，纤维蛋白胶不同的结合方式对细胞的增殖活性有一定的影响，3D-1复合模式更有利于细胞的存活增殖。

关键词: 纤维蛋白胶, 支架材料, 存活, 增殖

Abstract:

Objective This study explores the effects of different fibrin glue combination modes on the survival, proliferation, and apoptosis of dental follicle cells (DFCs), as well as to evaluate the feasibility and effectiveness of fibrin glue as transplantation material. Methods The membranes of surviving DFCs were marked using 3,3’-dioctadecyloxa carbocyanine perchlorate (DIO), and the cell number was counted by using ImageJ2x software. The apoptotic cells were marked with prodium iodide (PI). Results Compared with that of the 3D-2 and 2D-1 groups, the degradation speed of the 3D-1 group was the slowest. DFCs could survive and grow well in fibrinogen with a concentration of 15 mg•mL-1 supplemented with thrombin with a concentration of 2 U•mL-1. In particular, the 3D-1 combination mode was significantly conducive to cell proliferation and stretching. Conclusion Fibrin glue can be used as an effective cell transplantation material. The different combination modes have certain effects on cell proliferation. The 3D-1 combination mode is more conducive to the survival and proliferation of DFCs than other modes.

Key words: fibrin glue, support material, survival, proliferation

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