关键词: 骨形态发生蛋白-2, 基因, 骨髓间充质干细胞, 对氧化聚乙烯和聚丙烯共聚物, 牵张成骨, 新骨形成

Abstract:

Objective   To investigate the promotive effect of transplantation of bone morphogenetic protein-2(BMP-2) gene transfected bone mesenchymal stem cells(BMSCs) compounded with injectable bone tissue engineering scaffold material Pluronic F-127 on bone regeneration in rabbit mandibular distraction osteogenesis(DO). Methods     Forty-eight New Zealand’s white rabbits were randomized into four groups with twelve in each. All the objects were prepared into DO surgical model. On the 2nd day of consolidation, group A, B, C and D were injected with the same amount of 200 μL of the compound of BMP-2 gene transfected BMSCs with Pluronic F-127, the solution with BMP-2 gene trans-fected BMSCs, the solution of BMSCs and physiological saline at distraction zone, respectively. Two halves of the ob-jects of all groups were sacrificed at the end of 2nd and 6th week consolidation, respectively. And the specimens of right mandible were prepared for radiological, histomorphological and immunohistochemical examinations to evaluate bone regeneration. Results   Both radiological and immunohistochemical images were analyzed and processed with pro-fessional software. At the end of 2nd and 6th week consolidation, the bone mineral density and the expression of BMP-2 protein in distraction area of group A were significantly higher than those of B, C, D group(P<0.01). Group B was significantly higher than that in group C and D(P<0.01). There was no significant difference between group C and D(P>0.05). And the regeneration quality of distraction zone in group A and B were better than those in group C and D, just as that of group A better than group B. Conclusion   The transplantation of BMP-2 gene transfected BMSCs compounded with Pluronic F-127 could effectively pro-mote bone regeneration in rabbit mandibular DO.

Key words: bone morphogenetic protein-2, gene, bone mesenchymal stem cells, Pluronic F-127, dis-traction osteogenesis, bone regeneration