肌肉来源干细胞表面抗原-1阳性与阴性细胞体外培养成肌特性的比较

doi:10.3969/j.issn.1000-1182.2011.04.009

华西口腔医学杂志

肌肉来源干细胞表面抗原-1阳性与阴性细胞体外培养成肌特性的比较

车晓霞1 赵彤2 朱玲玲2 郭杰3

1.首都医科大学附属北京口腔医院正畸科，北京100050；2.军事医学科学院基础医学研究所脑保护与可塑性研究室，北京100850；3.山东大学口腔医院正畸科；山东省口腔生物医学重点实验室，济南250012

收稿日期:2011-08-25 修回日期:2011-08-25 出版日期:2011-08-20 发布日期:2011-08-20
通讯作者: 郭杰，Tel：0531-82778537
作者简介:车晓霞（1969—），女，河北人，副教授，博士
基金资助:
北京市自然科学基金资助项目（7072029）；山东省科技攻关基金资助项目（2007GG30002035）

A comparison of the myogenic characteristics between stem cell antigen-1 + and stem cell antigen-1- cells derived from skeletal muscle

Che Xiaoxia1, Zhao Tong2, Zhu Lingling2, Guo Jie3

1. Dept. of Orthodontics, School of Stomatology, Capital Medical University, Beijing 100050, China; 2. Dept. of Brain Protection and Plasticity, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China; 3. Dept. of Orthodontics, School of Stomatology, Shandong University, Jinan 250012, China; Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China）

Received:2011-08-25 Revised:2011-08-25 Online:2011-08-20 Published:2011-08-20
Contact: Guo Jie，Tel：0531-82778537

摘要/Abstract

摘要：

目的探讨干细胞表面抗原－1（Sca-1）在成肌细胞增殖分化中的作用。方法采用流式荧光细胞分选技术，从C57BL/6小鼠骨骼肌中分离Sca-1+与Sca-1-细胞进行体外培养，使用CCK-8试剂盒检测2种细胞的增殖曲线，Westernblot测定2种细胞在体外培养过程中Sca-1、MyoD、成肌素（Myogenin）的蛋白表达。结果在体外培养的前3 d，Sca-1+与Sca-1-细胞增殖曲线没有明显差别；3 d后，Sca-1-细胞增殖比Sca-1+细胞明显加速。同时，2种细胞在体外培养过程中Sca-1均没有明显表达，而Sca-1+细胞的成肌调节因子表达比Sca-1－细胞显著增加。结论Sca-1的表达在细胞生长发育过程中具有时段性，与成肌细胞细胞周期的起止有关。

关键词: 干细胞表面抗原-1, 肌肉干细胞, 成肌调节因子, 细胞周期

Abstract:

Objective To probe into the function of stem cell antigen-1（Sca-1） in cell proliferation and differentiation. Methods Sca-1+ and Sca-1- muscle derived cells were separated from C57BL/6 mice by fluorescence-activated cell sorting and then cultured in vitro. After 5 days cells proliferative curve were drawn according CCK-8 experimental results. Western blot also were done to detect Sca-1, MyoD and Myogenin expression in cultured Sca-1+ and Sca-1- muscle derived cells. Results The difference of the proliferative curve of Sca-1+ and Sca-1- muscle derived cells cultured 3 days in vitro was not apparent, but Sca-1- muscle derived cells had a accelerated division rate in the follow days compared to the Sca-1+ muscle derived cells. Sca-1 expression in both cells was not obvious. MyoD and Myogenin expression were stronger in Sca-1+ than Sca-1- muscle derived cells. Conclusion Sca-1 expression in muscle derived cells takes a period of time that related to the beginning and ending of the cell cycle.

Key words: stem cell antigen-1, muscle stem cell, muscle regulatory factor, cell cycle

车晓霞赵彤朱玲玲郭杰. 肌肉来源干细胞表面抗原-1阳性与阴性细胞体外培养成肌特性的比较[J]. 华西口腔医学杂志, doi: 10.3969/j.issn.1000-1182.2011.04.009.

Che Xiaoxia1, Zhao Tong2, Zhu Lingling2, Guo Jie3. A comparison of the myogenic characteristics between stem cell antigen-1 + and stem cell antigen-1- cells derived from skeletal muscle[J]. West China Journal of Stomatology, doi: 10.3969/j.issn.1000-1182.2011.04.009.

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肌肉来源干细胞表面抗原-1阳性与阴性细胞体外培养成肌特性的比较

A comparison of the myogenic characteristics between stem cell antigen-1 + and stem cell antigen-1- cells derived from skeletal muscle

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