关键词: 腭裂, 动物模型, 信号分子, 基因筛选

Abstract:

Objective To screen the wnt and fibroblast growth factor（FGF） ligands involved in palatogenesis and  cleft palate, and to study the dynamic expression of them in the different stages of palatal development and cleft  palate formation. Methods Mouse model of retinoic acid（RA）-induced cleft palate was set up. At embryo day（ED） 14.5, the palatal tissues of RA-treated group and wild type were collected and prepared for gene -chip analysis. According to the gene-chip results, wnt3, wnt8a, fgf9 and fgf10 were selected and their expression level was detected at ED13.5 -15.5 by using semi -quantitative reverse transcription -PCR（RT -PCR）. Results 1）Gene -chip analysis showed that in RA-induced cleft palate group wnt8a and fgf9 were down-regulated, wnt3 and fgf10 were up-regulated in conversely. 2）During the different stage of the control group palatogenesis, intense expression of wnt3, wnt8a, fgf9 and fgf10 were detected with a continuous dynamic pattern. 3）Compared with the control group, the expression level of wnt3, wnt8a, fgf9 and fgf10 in RA -induced cleft palate showed significant difference, respectively（P <0.05）. Conclusion wnt and FGF signaling molecules participate in the palatogenesis, and RA pathway may interact with wnt and FGF signaling pathway.

Key words: cleft palate, animal model, signaling pathway, gene screen