华西口腔医学杂志

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高迁移率族蛋白B1对牙周膜成纤维细胞表达细胞因子影响的研究

孙钦峰1 徐岩2 宋晖1 扈英伟1 杨丕山1   

  1. 1.山东大学口腔医院牙周科, 山东济南250012; 2.济南市口腔医院东院口腔内科, 山东济南250014
  • 收稿日期:2010-08-25 修回日期:2010-08-25 出版日期:2010-08-20 发布日期:2010-08-20
  • 通讯作者: 杨丕山,Tel:0531-88382368
  • 作者简介:孙钦峰(1967—),男,山东人,副教授,博士
  • 基金资助:

    国家自然科学基金资助项目(30772425);山东省科技攻关基金资助项目(2009GG10002052)

Effects of high mobility group box 1 in activating periodontal ligament fibroblasts to express cytokine

SUN Qin-feng1, XU Yan2, SONG Hui1, HU Ying-wei1, YANG Pi-shan1   

  1. 1. Dept. of Periodontology, School of Stomatology, Shandong University, Jinan 250012, China; 2. Dept. of Oral Medicine, East Branch of Jinan Stomatologic Hospital,Jinan 250014, China
  • Received:2010-08-25 Revised:2010-08-25 Online:2010-08-20 Published:2010-08-20
  • Contact: YANG Pi-shan,Tel:0531-88382368

摘要:

目的观察高迁移率族蛋白B1(HMGB1)对人牙周膜成纤维细胞表达白细胞介素-6(IL-6)、破骨细胞核因子κB受体活化因子(RANKL)、骨保护因子(OPG)的影响,初步探讨HMGB1在牙周疾病的作用。方法采用原代组织块培养法,培养人牙周膜成纤维细胞,用第4~6代的细胞进行实验。分别用10、30、100 ng·mL-1质量浓度的HMGB1孵育牙周膜成纤维细胞24 h后,RT-PCR检测IL-6、RANKL、OPG的mRNA表达;Western blot法检测RANKL、OPG的蛋白表达。均以0 ng·mL-1质量浓度组为对照,所得数据用单因素方差分析处理。结果HMGB1在10、30、100 ng·mL-1质量浓度时,细胞中的RANKL/OPG mRNA的比值增高(P<0.05),100 ng·mL-1质量浓度时细胞中的IL-6 mRNA的表达量增高(P<0.05)。Western blot检测结果显示10 ng·mL-1质量浓度组的RANKL/OPG的比值有明显增高。结论一定浓度的HMGB1可使牙周膜细胞中的RANKL/OPG比值增高,还会诱导炎症因子IL-6 mRNA表达上调。提示HMGB1可能会在牙周炎的发病以及炎症进展中发挥作用。

关键词: 高迁移率族蛋白B1, 牙周膜成纤维细胞, 白细胞介素-6

Abstract:

Objective To investigate the influence of high mobility group box 1(HMGB1) on the expression of interleukin 6(IL-6), receptor activator of nuclear factor-kappa B ligand(RANKL) and osteoprotegerin(OPG) on periodontal ligament fibroblasts. Methods Human periodontal ligament fibroblasts were stimulated with HMGB1 at concentrations of 10, 30, and 100 ng·mL-1 for 24 h. RT-PCR and Western blot analysis were performed to check mRNA and protein expression of IL-6, RANKL and OPG on the cells. Results The ratio of RANKL/OPG was increased at both mRNA and protein level after HMGB1 stimulation at 10, 30, 100 ng·mL-1. Inflammatory cytokine IL-6 was upregulated by HMGB1 at the concentration of 100 ng·mL-1. Conclusion Increased ratio of RANKL/OPG and IL-6 on periodontal ligament fibroblasts suggests that HMGB1 might play a role in the pathogenesis and progression of periodontal disease.

Key words: high mobility group box 1, periodontal ligament fibroblasts, interleukin-6