Please wait a minute...
Table of Content
01 October 2017, Volume 35 Issue 5
  • Orginal Article
    Collaboration from periodontics in the aesthetic restoration of anterior teeth
    Qintao Wang, Yumei Zhang
    West China Journal of Stomatology. 2017, 35(5):  453-455.  doi:10.7518/hxkq.2017.05.001
    Abstract ( 782 )   HTML ( 37 )   PDF(pc) (705KB) ( 963 )   Save
    References | Related Articles | Metrics

    Anterior teeth are the main zones that reflect oral aesthetics. The histological structure, outside appearance, relative ratios, and other characteristics of tissues must be understood to obtain relatively ideal and natural restoration effects. A collaboration of different disciplines must be implemented to obtain harmonious and stable restoration outcomes that help the majority appreciate beauty. This article aims to discuss the role of periodontics in this collaboration and introduce several common techniques.

    Treatment of complex crown-root fracture
    Ying Zhang
    West China Journal of Stomatology. 2017, 35(5):  456-460.  doi:10.7518/hxkq.2017.05.002
    Abstract ( 1186 )   HTML ( 47 )   PDF(pc) (1028KB) ( 1451 )   Save
    Figures and Tables | References | Related Articles | Metrics

    With the development of the oral clinical technology and materials, the concept of complex crown root fracture has become more scientific and developed. Epidemiological characteristics, causes and clinical features of this disease are generalized. Aesthetic, functional and minimally invasive coordination is reflected by expounding the diagnosis of complex crown-root fracture, recommendation of treatment and techniques, and characteristics of immature permanent teeth to syste-matize the treatment strategy, and to guide the clinical therapy of complex crown-root fracture.

    Preparation processes of 3% yttria-stabilized tetragonal zirconia polycrystalline ceramic composite powders coated with nano-sized Al2O3-boron nitride
    Jinshuang Wu, Lei Zhou, Helin Xing, Zhe Wang, Chaofang Dong, Sefei Yang
    West China Journal of Stomatology. 2017, 35(5):  461-467.  doi:10.7518/hxkq.2017.05.003
    Abstract ( 845 )   HTML ( 12 )   PDF(pc) (1923KB) ( 568 )   Save
    Figures and Tables | References | Related Articles | Metrics

    Objective In this work, we aim to determine the optimum pH value for the preparation of 3% yttria-stabilized tetragonal zirconia polycrystalline (3Y-TZP)/3%Al2O3 and optimum calcination temperature of Al2O3 precursor and amorphous boron nitride (BN) for Al2O3-BN coating 3Y-TZP powders. Methods The 3Y-TZP/3%Al2O3 composite powders were prepared through the heterogeneous precipitation method under different pH values (6.5, 7.5, 8.5, and 9.5) and analyzed through trans-mission electron microscopy (TEM) to determine the optimum coating morphology. Al2O3 precursor, which was prepared under the optimum pH value, was calcined at different temperatures (800, 1 000, 1 200, and 1 400 ℃). The amorphous BN coating 3Y-TZP/3%Al2O3 powder was prepared via in situ reaction with boric acid and urea (calcined with N2 at 850 ℃ for 5 and 3 h and 800 ℃ for 5 and 3 h). TEM, energy dispersive spectrometry (EDS), and X-ray diffraction (XRD) were performed to characterize the results. Results The optimum coating morphology was obtained at 8.5 pH. When the Al2O3 precur-sor was calcined at 1 200 ℃ and coated with BN (calcined with N2 at 800 ℃ for 5 h), the Al-B-O compound was less, and t-ZrO2 was more represented. Conclusion The optimum pH value for 3Y-TZP/3%Al2O3 composite preparation is 8.5. For the preparation of Al2O3-BN coating 3Y-TZP powders, the optimum calcination temperature of the Al2O3 precursor and amorphous BN are 1 200 and 800 ℃, respectively.

    Construction of a eukaryotic expression vector of fibroblast activation protein and establishment of its stable over-expression in the oral squamous cell carcinoma
    Meng Zhao, Tingru Shao, Jiaxin Huang, Yuechuan Chen, Xiaozhi Lü
    West China Journal of Stomatology. 2017, 35(5):  468-472.  doi:10.7518/hxkq.2017.05.004
    Abstract ( 726 )   HTML ( 7 )   PDF(pc) (1365KB) ( 501 )   Save
    Figures and Tables | References | Related Articles | Metrics

    Objective This study aimed at constructing fibroblast activation protein (FAP) over-expression lentivinus vectors to investigate transfection in SCC9 cell lines and establish a stable FAP over-expression oral squamous cell line. Methods The cDNA of FAP gene from an oral squamous cell carcinoma (OSCC) tissue was amplified by polymerase chain reaction (PCR) and subcloned into eukaryotic expression vector pCDH-CMV-MCS-EF1-copGFP. The recombinant plasmid was sequenced and then transfected into an SCC9 cell line. Subsequently, the SCC9 cell line that over-expressed FAP stably was established by fluorescence activated cell sorting (FACS). The expression of green fluorescent protein (GFP) was detected with fluorescence microscopy, and the over-expression of FAP was identified by real-time PCR and Western blot. Results The FAP gene was amplified by PCR and then cloned into the vector, whose sequence was identical to that in the GenBank. GFP was expressed in the transfected cells. Furthermore, FAP over-expression in the transfected cells was detected by real-time PCR and Western blot. Conclusion The recombinant eukaryotic expression vector pCDH-FAP was constructed success-fully. This result provides a foundation for further studies on the function of FAP in vitro.

    Role of adenosine triphosphate-sensitive potassium channel in hydrogen sulfide-induced inhibition of high glucose-induced osteoblast damage
    Yuanyuan Liu, Xiumei Guan, Min Cheng, Xin Li, Yueyang Pan, Zhiliang Guo
    West China Journal of Stomatology. 2017, 35(5):  473-478.  doi:10.7518/hxkq.2017.05.005
    Abstract ( 633 )   HTML ( 8 )   PDF(pc) (1064KB) ( 473 )   Save
    Figures and Tables | References | Related Articles | Metrics

    Objective The aim of this study is to identify the role of adenosine triphosphate-sensitive potassium channel (KATP) in hydrogen sulfide (H2S)-induced inhibition of high glucose (HG)-induced osteoblast damage. Methods Osteoblasts from rat mandible were cultured and identified. The osteoblasts were then treated with HG, H2S, KATP channel opener pinacidil (Pia), and KATP channel blocker glibenclamide (Gli). Western blot method was performed to detect the expression of KATP channel protein. CCK8, reverse transcriptase polymerase chain reaction (RT-PCR) , and image analysis were used to determine the effects of H2S-KATP on the proliferation, differentiation, and mineralization of osteoblasts. Results The expression of KATP channel protein in osteoblasts was significantly decreased under the influence of HG. H2S pretreatment significantly inhibited HG on KATP channel protein down-regulation. Moreover, H2S pretreatment significantly inhibited the effect of HG on the proliferation of osteoblasts, thereby preventing HG-induced inhibition of osteoblasts differentiation and mineralization. Meanwhile, the KATP channel blocker effectively blocked the H2S on osteoblasts and had a pro-tective effect. Conclusion Through the KATP channel, H2S inhibited osteoblasts damage induced by HG.

    Role of CCL5/CCR5 in the perineural invasion of salivary adenoid cystic carcinoma cells
    Tianyi Li, Zhiyuan Shen
    West China Journal of Stomatology. 2017, 35(5):  479-483.  doi:10.7518/hxkq.2017.05.006
    Abstract ( 670 )   HTML ( 32 )   PDF(pc) (1095KB) ( 467 )   Save
    Figures and Tables | References | Related Articles | Metrics

    Objective This study aimed the role of the CCL5/CCR5 axis in the perineural invasion of salivary adenoid cystic carcinoma (SACC) cells. Methods Immunohistochemical analysis and flow cytometric analysis were conducted to detect the expression of the chemokine receptor CCR5 in SACC cells. Enzyme linked immunosorbent assay (ELISA) was performed to determine the expression of CCL5 in the supernate of human nerve cells. The flow cytometric analysis was applied to observe the changes in F-actin in SACC-LM cells, which were pretreated with CCL5. To assess the effects of the CCL5/CCR5 axis on the migration and invasion of SACC-LM cells, we performed a scratch test and invasion assay under CCL5 stimulation. Results CCR5 was highly expressed in SACC cells. The concentration of CCL5 in the supernatant of human nerve cells was (359.2±15.8), (696.4±22.6) pg·mL-1. The CCL5/CCR5 axis promoted the migration and invasion of SACC-LM cells. Conclusion The CCL5/CCR5 axis may be involved in the perineural invasion of SACC cells.

    Evaluation for vertical stability after various orthognathic surgical treatment plans for skeletal class Ⅲ malocclusion with anterior open-bite
    Xianwen Liu, Weijian Ai, Huixi Zhou, Yunfeng Li, Shuguang Liu
    West China Journal of Stomatology. 2017, 35(5):  484-488.  doi:10.7518/hxkq.2017.05.007
    Abstract ( 788 )   HTML ( 23 )   PDF(pc) (852KB) ( 739 )   Save
    Figures and Tables | References | Related Articles | Metrics

    Objective To evaluate vertical stability after various orthognathic surgical treatment plans for skeletal class Ⅲ malocclusion with anterior openbite. Methods A retrospective multicenter cohort study was conducted to investigate vertical stability after various orthognathic surgical treatment plans for skeletal class Ⅲmalocclusion with anterior openbite. From 2010-2016, 122 patients from two domestic stomatological hospitals were included in our study. Patients were divided into four groups according to their treatment plans, namely, bilateral sagittal split ramus osteotomy (BSSRO), intraoral vertical ramus osteotomy (IVRO), BSSRO+Le FortⅠ, and IVRO+Le FortⅠ. All patients followed a standardized examination procedure at 6 and 24 months post-treatment. The observation indexes include overbite, mandibular plane angle, and intermaxillary angle. Results 1) The significantly reduced ratio of the overbite in the BSSRO+Le FortⅠand IVRO+Le FortⅠgroups were less than the BSSRO and IVRO groups at 6 and 24 months post-treatment. 2) The significantly increased ratio of the mandibular plane in BSSRO+Le FortⅠand IVRO+Le FortⅠgroups were less than BSSRO and IVRO groups at 6 and 24 months post-treatment. 3) The significantly increased ratio of the intermaxillary angles in BSSRO+Le FortⅠand IVRO+Le FortⅠgroups were less than the BSSRO and IVRO groups at 6 months post-treatment, while there was no stati-stical difference at 24 months post-treatment. Conclusion Bimaxillary surgery (BSSRO+Le FortⅠand IVRO+Le FortⅠ) is more effective than mandibular surgery to control vertical relapse.

    Effect of body image in adolescent orthodontic treatment
    Minghui Peng, Jing Kang, Xiao Deng
    West China Journal of Stomatology. 2017, 35(5):  489-493.  doi:10.7518/hxkq.2017.05.008
    Abstract ( 739 )   HTML ( 8 )   PDF(pc) (813KB) ( 543 )   Save
    Figures and Tables | References | Related Articles | Metrics

    Objective This study was designed to probe the psychological factors adolescent orthodontic patients, the role of body image and self-esteem in the whole process of orthodontic treatment and the impact on the efficacy and satisfaction of orthodontic. Methods Five hundred and twenty-eight patients were selected in this study. The Aesthetic Component of the Index of Orthodontic Treatment Need (IOTN-AC) , Rosenberg Self-Esteem Scale (SES), Negative Physical Self-General (NPS-G) and other body analysis scale study after orthodontic lasted 18-24 months were used to investigate the role of body image in adolescent orthodontic treatment. Results Esthetic evaluation of patients teeth after correction had been significantly improved, patient self-evaluation difference IOTN-AC doctor evaluation, Psychosocial Impact of Dental Aesthetics Question-naire-tooth confidence, aesthetic concerns, psychological impact and social function were significantly improved. The improve-ment of the dental aesthetics component (T2 when doctors evaluate IOTN-AC) was positively correlated with the evaluation of the efficacy, and was significantly negatively correlated with the negative emotions of patients at baseline. Conclusion Negative body image-dental dissatisfied-cognitive component and the affective component, the overall negative body image and negative emotions can predict patient satisfaction with treatment efficacy. Orthodontic treatment not only improves the self-aesthetic evaluation of adolescent patients, but also has a positive effect on the mental health of adolescent patients.

    Study on using laser Doppler flowmetry to determine the pulpal blood flow of immature maxillary incisors
    Ninghu Ma, Gang Xiao, Yuanyuan Zhang, Lin Zhang, Shuyang He, Shanshan Dai, Yangcheng Li, Qingyu Guo
    West China Journal of Stomatology. 2017, 35(5):  494-497.  doi:10.7518/hxkq.2017.05.009
    Abstract ( 1004 )   HTML ( 12 )   PDF(pc) (922KB) ( 576 )   Save
    Figures and Tables | References | Related Articles | Metrics

    Objective This study aimed to evaluate the influence of age on the pulpal blood flow (PBF) of immature maxil-lary incisors of maxillary incisors, which was detected by laser Doppler flowmetry (LDF). Methods LDF was used to detect the PBF value of maxillary central and lateral incisors of a child group (aged 7-13 years old) and a positive control group (aged 18-25 years old), as well as the central incisor of a negative control group (the central incisor had undergone endodontic treatment). We then compared the features of PBF in all groups with the influence of gender and position on PBF. The relation of maxillary central incisor and lateral incisor, age, and maxillary incisor were analyzed. Results The PBF value of the nega-tive control group was (2.08±0.73) PU. The PBF values in the positive control group in central and lateral incisors were (8.49±1.88) and (7.52±1.82) PU. In the child group, PBF values in central incisors and lateral incisors were (11.31±2.21) and (12.18±2.65) PU. A significant difference was observed between different groups and between central and lateral incisors (P<0.01). Meanwhile, no significant difference was found in the PBF values between the right and the left parts in both males and females (P>0.05). Age had a linearity negative correlation with the PBF value of incisors in the child group. A linear nega-tive correlation existed between the age and PBF of central and lateral incisors (r=-0.310 and r=-0.510, respectively) (P<0.01). Conclusion PBF value decreased with increased age in children aged 7-13 years old.

    Effect of osseointegration in the posterior area by Shenggu pills
    Cuifen Luo, Guoguang Peng
    West China Journal of Stomatology. 2017, 35(5):  498-501.  doi:10.7518/hxkq.2017.05.010
    Abstract ( 1068 )   HTML ( 8 )   PDF(pc) (784KB) ( 672 )   Save
    Figures and Tables | References | Related Articles | Metrics

    Objective This study aimed to investigate the osseointegration condition by measuring the implant stability quotient (ISQ) to study the effect of osseointegration in the posterior area by Shenggu pills. Methods A total of 101 DENTIUM implants were placed in 48 patients. Fifty implants placed in 24 patients were addressed as usual, whereas 51 implants placed in 24 patients were treated with Shenggu pills. ISQ was tested on the day of surgery and consecutively at 4, 8, and 12 weeks. All data were analyzed. Results The efficacy of the treatment group was no much better than that of control group on the day of surgery and consecutively at 12 weeks with no significant difference (P>0.05). The efficacy of the treatment group was significantly better than that of the control group at 4 and 8 weeks (P<0.05). The implants placed in the mandible were significantly better than those placed in the maxilla (P<0.05). Conclusion Early intake of Shenggu pills could accelerate the implant bone union, improve the implants’ ISQ, and ensure the success rate of the operation.

    Application of temporomandibular joint dics reduction in the operation of condylar sagittal fracture
    Wenli Zeng, Wuchao Zhou, Jingkun Zhang, Yisen Shao, Weihong Xi
    West China Journal of Stomatology. 2017, 35(5):  502-505.  doi:10.7518/hxkq.2017.05.011
    Abstract ( 781 )   HTML ( 5 )   PDF(pc) (814KB) ( 646 )   Save
    Figures and Tables | References | Related Articles | Metrics

    Objective To explore the selection of temporomandibular joint (TMJ) disc reduction and fixation methods in condylar sagittal fracture surgery. Methods A total of 36 patients with condylar fractures were chosen. The follow-up period was more 6 months. All 36 cases of condylar sagittal fracture were fixed with long screw. In the operation, the displaced joint disc was repositioned and fixed. The fixed method included direct suture (22 cases) and anchorage (14 cases). Clinical followups were performed before surgery and 1 month, 3 months, 6 months and 1 year after surgery. Clinicians recorded data related to the Fricton craniomandibular index (CMI) and evaluated the postoperative joint function during followup before surgery and 6 months after surgery. Results In both groups, function of TMJ significantly improved after surgery. The CMI decreased from 0.213±0.162 and 0.273±0.154 to 0.059±0.072 and 0.064±0.068 (P<0.05), respectively. No statistical difference was observed between the two groups in palpation index (PI), dysfunction index (DI) and CMI (P>0.05) before or after surgery. Conclusion Both methods could effectively improve the dysfunction of the TMJ caused by trauma. The selection of joint disc reduction and fixation methods is based on the displacement and damage degree of the joint disc.

    Experimental study on accelerated healing of jaw fracture using gelatin sponge compound growth factor
    Yan-feng Tang, Jianlin Chen, Yunbiao Zhou
    West China Journal of Stomatology. 2017, 35(5):  506-509.  doi:10.7518/hxkq.2017.05.012
    Abstract ( 795 )   HTML ( 9 )   PDF(pc) (892KB) ( 663 )   Save
    Figures and Tables | References | Related Articles | Metrics

    Objective To explore the role and mechanism of drug delivery systems using growth factor combined with gelatin sponge on accelerating the healing of jaw fracture and to seek better treatment of accelerating the maxillofacial fracture. Methods About 100 μg recombinant human bone morphogenetic protein (BMP)-2 was completely dissolved in 1 mL recombi-nant bovine basic fibroblast growth factor (bFGF), and the solution (40 μL) was dropped in gelatin sponge (0.5 cm×0.5 cm×1.0 cm). Then, it was freeze dried and prepared into bFGF/BMP/gelatin sponge delivery systems. The mandibular fracture model on two sides were prepared in 12 New Zealand rabbits and randomly divided into two groups. The left side was the control group, which was only fixed with titanium plates. The right side was the experimental group, in which bFGF/BMP/gelatin sponge delivery systems were put under the titanium plates. General observation, X-ray, and histological examination were taken at 2, 4, and 12 weeks after surgery. Results After 2 weeks, more fibrous tissues were seen between the fracture ends in the experimental group than in the control group. After 4 weeks, fibrous fracture callus were seen in the fracture gap in the experimental group. The ingrowths of fibrous tissue and blood vessels were seen in the control group. The fracture healing of the experimental group was significantly faster than the control group at 2 and 4 weeks. After 12 weeks, the experimental and control groups all healed completely. Conclusion bFGF/BMP/gelatin sponge can accelerate and improve fracture healing; thus, it has better clinical application prospect.

    Application of bone grafts from chin of the mandible in the reconstruction of orbital fracture
    Xiaoyu Li, Jing Wu, Xinya Du, Jian Huang, Bin Wu, Chun Xie
    West China Journal of Stomatology. 2017, 35(5):  510-513.  doi:10.7518/hxkq.2017.05.013
    Abstract ( 887 )   HTML ( 6 )   PDF(pc) (820KB) ( 404 )   Save
    Figures and Tables | References | Related Articles | Metrics

    Objective To evaluate the clinical effects of bone graft from the mandible in repairing orbital floor defects. Methods Bone grafts from the mandible were used to treat 11 cases of orbital floor defects and followed up for 6-12 months. Results The surgical incisions healed primarily in all 11 patients. The orbital floor structure was restored. No vision loss, limited eye movement, implant infection, or resorption were observed postoperatively, and no complications occurred in the supply area. Conclusion Bone grafts from the mandible were an ideal material to repair orbital floor defects.

    Correlation between interleukin-10 polymorphisms and susceptibility to chronic periodontitis among Uygur adults in the Moyu area
    Yuhui Zhang, Ping Huang, Jing Lin, Jin Zhao
    West China Journal of Stomatology. 2017, 35(5):  514-519.  doi:10.7518/hxkq.2017.05.014
    Abstract ( 601 )   HTML ( 12 )   PDF(pc) (899KB) ( 419 )   Save
    Figures and Tables | References | Related Articles | Metrics

    Objective This study aims to investigate the association between interleukin (IL)-10-597 (C/A) single-nucleotide polymorphisms and chronic periodontitis of Moyu Uygur population in Xinjiang Uygur Autonomous Region of China. Methods In accordance with the inclusion and exclusion criteria, the buccal swabs of 300 subjects were randomly selected from the epidemiological investigation of Uygur adults in Moyu county on April and May 2013. The study was conducted on a healthy control group, a mild chronic periodontitis group, and a moderate-to-severe chronic periodontitis group, with each comprising 100 samples. The IL-10-597(C/A) site in the promoter sequences was analyzed using the tetra-primer amplification refractory mutation system-polymerase chain reaction method to test the genotype and allele distributions. Statistical analysis was performed using Chi-squared test and ordinal classification Logistic regression analysis. Results The genotype and allelefrequencies of the IL-10-597(C/A) site in the healthy control group, mild chronic periodontitis group, and moderate-to-severe chronic periodontitis group exhibited no significant difference (P>0.05). The age of all the samples was associated with chronic periodontitis. The risk of chronic periodontitis in the people of 55-65 years old was 25 times in the people under the age of 35 (OR=25.56, P<0.001). Conclusion The IL-10-597 (C/A) single-nucleotide polymorphisms in the gene promoter are not associated with chronic periodontitis in Uygur adult population.

    The role of extracellular signal regulated kinase 1/2 in mediating osteodifferentiation of human periodontal ligament cells induced by cyclic stretch
    Jing Song, Dapeng Ren, Shiguo Yan, Jing Lan, Xiao Yuan, Qingyuan Guo, Xiang-min Qi
    West China Journal of Stomatology. 2017, 35(5):  520-526.  doi:10.7518/hxkq.2017.05.015
    Abstract ( 536 )   HTML ( 12 )   PDF(pc) (1307KB) ( 410 )   Save
    Figures and Tables | References | Related Articles | Metrics

    Objective This study aimed to investigate the mechanism of cyclic stretch that promotesthe osteogenic diffe-rentiation of human periodontal ligament cells (hPDLCs) through the mediation of extracellular-signal-regulated kinase 1/2 (ERK1/2). Methods hPDLCs were isolated throughthe explant method and cultured in vitro. hPDLCs were mechanically stimulated by a multi-channel cell-stress-loading system for 1, 3, 6, 12, and 24 h. The magnitude of stretch was 10% defor-mation, and the frequency was 0.5 Hz. Nonloaded cells were used as control group. ERK1/2 activation was blocked by U0126, a specific ERK1/2 pathway inhibitor. Additionally, hPDLCs were transfected with adenoviral vector encoding dominant negative ERK1/2 (DN-ERK1/2) to continuouslyinhibit ERK1/2 activation. The mRNA and protein levels of target geneswere detected through real-time polymerase chain reaction and Western blot. Results Cyclic stretching promoted the expression of ERK1/2, osteocalcin (OCN) mRNA, and bone sialoprotein (BSP) mRNA. The expression of runt-related transcription factor (Runx) 2 protein and mRNA also increased at 3 and 6 h of cyclic stretching. The inhibition of ERK1/2 by U0126 and DN-ERK1/2 suppressed the expressionof Runx2 mRNA, OCN mRNA, BSP mRNA, Runx 2 protein, and p-ERK1/2 protein relative to that in stretched cells without the ERK1/2 inhibitor. Conclusion ERK1/2 is a critical molecule in the mediation ofthe osteogenic differentiation of hPDLCs under mechanical stimulation. ERK1/2 activation induced the elevation of Runx2 protein levels, which may be involved in the stretch-induced expressions of OCN and BSP.

    Research progress on the relationship and mechanisms between periodontal disease and preterm birth and low-birth-weight infants
    Wanhong Wu, Changchang Ye, Ping Huang
    West China Journal of Stomatology. 2017, 35(5):  527-532.  doi:10.7518/hxkq.2017.05.016
    Abstract ( 654 )   HTML ( 33 )   PDF(pc) (787KB) ( 520 )   Save
    References | Related Articles | Metrics

    Preterm birth (PB) is defined as the birth of a baby less than 37 weeks of gestational age. Low birth weight (LBW) is defined as a newborn baby’s weight of less than 2 500 g. PB is often accompanied by LBW. Preterm low birth weight (PLBW) is the leading cause of newborn deaths. Periodontal disease (PD) is a chronic oral infectious disease, and it is closely related with general health. Epidemiological data show that PD is a risk factor for PLBW and other adverse pregnancy outcomes. The possible mechanisms include the direct effects of periodontal bacteria, inflammatory reactions, and immune response; however, the exact pathogenetic mechanism involved remains controversial. This article aims to review the research progress on the relationship between PD and PLBW and their underlying mechanisms, as well as the effects of periodontal treatment on PLBW incidence.

    Clinical applications of stem cells from human exfoliated deciduous teeth in stem cell therapy
    Xiaoxia Li, Jiaozi Fangteng, Shi Yu, Yuming Zhao, Lihong Ge
    West China Journal of Stomatology. 2017, 35(5):  533-537.  doi:10.7518/hxkq.2017.05.017
    Abstract ( 1330 )   HTML ( 28 )   PDF(pc) (849KB) ( 1104 )   Save
    References | Related Articles | Metrics

    Stem cells from human exfoliated deciduous teeth (SHED) are one category of dental stem cells. They belong to ectodermal mesenchymal stem cells. As an ideal stem cell source, SHED possess great potential in stem cell therapy. This review demonstrates the biological characteristics and advantages of SHED in stem cell therapy and discusses its multiple functions in tissue regeneration and repair, including multiple differentiation potentiality, cell secretion of cytokines, and immunomodulatory ability. Furthermore, this article introduces the main findings regarding the potential clinical applications of SHED to a variety of diseases. This article demonstrates research progress in dentin-pulp regeneration, maxillofacial bone regeneration, and treatment of nervous system and immune system diseases with SHED for stem cell transplantation.

    Research progress on the type Ⅸ secretion system of Porphyromonas gingivalis
    Jinmei Zhang, Lei Zhao, Yafei Wu
    West China Journal of Stomatology. 2017, 35(5):  538-542.  doi:10.7518/hxkq.2017.05.018
    Abstract ( 856 )   HTML ( 13 )   PDF(pc) (774KB) ( 627 )   Save
    References | Related Articles | Metrics

    In recent years, the study found that Porphyromonas gingivalis type Ⅸ secretion system (T9SS) is a novel protein secretion system, also known as Por secretion system (PorSS). Unlike the eight protein secretion systems found in the past, the system is a polyprotein complex found only in Bacteroides. The secreted proteins have both N- and C-terminus, where the former includes Sec-dependent typeⅠsignals peptide, and the latter contains conserved domains (C-terminal conserved domain, CTD). Porphyromonas gingivalis T9SS includes proteins such as intima, outer membrane, cytoplasm, and cell cycle, including at least 34 proteins containing CTD. Porphyromonas gingivalis T9SS is involved in regulating associated virulence factors including gingivin, fimbriae, lipopolysaccharide, HBP35, CPG70 protein and peptidyl-arginine deiminase. These CTD-containing virulence proteins are localized by T9SS and then released to the extracellular domain, thereby destroying periodontal tissue. Therefore, this review summarizes the research progress on the T9SS of Porphyromonas gingivalis.

    Research progress on QMix properties in root canal irrigation
    Jiayi Wu, Ruijie Huang
    West China Journal of Stomatology. 2017, 35(5):  543-548.  doi:10.7518/hxkq.2017.05.019
    Abstract ( 1145 )   HTML ( 24 )   PDF(pc) (710KB) ( 1011 )   Save
    References | Related Articles | Metrics

    Mechanical instrumentation preparation alone is insufficient to completely remove root canal infection, and chemical irrigation is essential to eliminate infected remnants. An ideal root canal irrigant should completely remove the smear layer, lubricate the root canal, efficiently kill bacteria, induce mild or no inflammatory response in the tissues, and avoid damaging the dentin structure. However, a commercial irrigant that meets all these requirements is currently lacking. QMix is a root canal irrigation mixture of ethylenediamine tetraacetic acid (EDTA), chlorhexidine (CHX) and surfactant. This mixture can remove the smear layer efficiently, and it possesses strong antibacterial effect and good biocompatibility with minimal cytotoxicity. Furthermore, the influence of QMix on the color and micro-hardness of dentin is low, and it can improve the wettability of root canal sealant without affecting its adhesive properties. This review compares the efficiencies of QMix with other irrigants (sodium hypochlorite, CHX, EDTA, SmearClear, and MTAD) in term of smear layer removal, dentine and root canal sealing, cell cytotoxicity, and bacterial growth inhibition.

    Taste signal transduction and the role of taste receptors in the regulation of microbial infection
    Yangyu Lu, Ranhui Xi, Xin Zheng, Jinzhi He, Xin Xu
    West China Journal of Stomatology. 2017, 35(5):  549-554.  doi:10.7518/hxkq.2017.05.020
    Abstract ( 704 )   HTML ( 13 )   PDF(pc) (791KB) ( 678 )   Save
    References | Related Articles | Metrics

    Taste receptors guide individuals to consume nutrients while avoiding potentially noxious substances. Interes-tingly, recent studies have shown that taste receptors are also expressed beyond the taste buds, including brain, respiratory system, and digestive system, etc. These extragustatory taste receptors play important roles in microbial infection, nutrient uptake and host homeostasis. Mang extragustatory taste receptors have been proposed to sense microorganisms and regulate host innate defense. More importantly, polymorphisms of genes encoding taste receptor, particularly bitter taste receptor, are linked to different innate defensive responses. This review introduces the molecular basis of taste signal transduction, and the role of taste receptors in the regulation of innate immunity during microbial infection were further discussed in detail.

    Features of condylography curves
    Hanchi Zhao, Tingting Lan, Bilu Xiang, Zhongjie Li, Yang Liu
    West China Journal of Stomatology. 2017, 35(5):  555-560.  doi:10.7518/hxkq.2017.05.021
    Abstract ( 1043 )   HTML ( 29 )   PDF(pc) (1460KB) ( 978 )   Save
    Figures and Tables | References | Related Articles | Metrics

    With the kinematic face bow, the condyle movements representing temporomandibular joint function can be recorded instantly. The condylography curves can be utilized for diagnostic analysis, as different pathophysiological conditions have unique curve patterns. By tracing the specific alterations on the curves, the structural and functional statuses of the tem-poromandibular joint can be predicted. However, to the best of our knowledge, no standardized and practicable evaluation protocol for jaw movement tracing exists. In the current study, the features of either normal or abnormal curves were reviewed. Such features can be clinically used for determining temporomandibular joint status, thereby contributing to a comprehensive diagnosis.

    Synchronous carcinoma of the floor of mouth carcinoma with esophageal carcinoma: a case report
    Hua Su, Xin-cheng Guo, Haiqing Wang
    West China Journal of Stomatology. 2017, 35(5):  561-563.  doi:10.7518/hxkq.2017.05.022
    Abstract ( 797 )   HTML ( 8 )   PDF(pc) (823KB) ( 517 )   Save
    Figures and Tables | References | Related Articles | Metrics

    This study presents a case of synchronous multiple primary cancers involving floor of mouth carcinoma with esophageal carcinoma. Literature was reviewed to summarize the incidence, location, diagnosis, treatment characteristics, and prognosis to improve understanding and awareness of the multiple primary cancer. As a result, early discovery, early diagnosis, and effective treatment can help prolong survival and improve the quality of life of patients.