Cemental dysplasia is a non-neoplastic lesion and features different X-ray appearances that easily confuse dentists. This condition is often observed in conventional dental films or occasionally on panoramic radiographs. Cemental dysplasia is easily misdiagnosed as chronic periapical periodontitis, osteomyelitis, and tumor. In this paper, clinical mani-festations, imaging diagnosis, and the correlation between these variables are analyzed to achieve correct diagnosis.

The field of periodontics has become increasingly discussed and advanced in recent decades. The techniques of periodontal soft tissue grafts are among these advancements. The techniques have been used more and more clinically not only in periodontics, but also in the areas of dental implantology, aesthetics, and orthodontics. In this article, we discuss the clinical indications and the advantages and disadvantages of several autogenous tissue grafts (e.g. pedical grafts, free gingival graft, and subepithelial connective tissue graft). These techniques have made root coverage and the rebuilding of attached gingival possible; therefore, it is necessary for all dentists to understand the applications of periodontal soft tissue grafts.

Objective RNA interference was used to silence geranylgeranyltransferase Ⅰ(GGTase-Ⅰ) in vitro and to study the effect of GGTase-Ⅰ on the migration and invasion of tongue squamous cancer cells. Methods Three small interfering RNAs (siRNA) were designed according to the GGTase-Ⅰ sequence by Genebank and were transfected into tongue squamous cancer cells Cal-27 to knock down GGTase-Ⅰ expression. The tested cells were divided into three groups, as follows: the RNA-interfered groups (GGTase-Ⅰ siRNA1, GGTase-Ⅰ siRNA 2, GGTase-Ⅰ siRNA 3), a negative control group (disrupted by random sequence NC-siRNA), and a blank control group. GGTase-Ⅰ and RhoA gene expressions were examined by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. The optimum interference group was screened by qRT-PCR and Western blot and was assigned as the experimental group. Matrix metalloproteinase (MMP)-2 and MMP-9 protein expressions were examined by Western blot. GTP-RhoA expression of protein was examined by GST-pull down. The migration and invasion abilities were analyzed by wound healing assay and Transwell motility assay. Results GGTase-Ⅰ mRNA and protein expression in Cal-27 decreased significantly after transfection of GGTase-I siRNA (P<0.05). No significant difference of RhoA gene expression was detected. MMP-2, MMP-9, and GTP-RhoA protein expressions decreased significantly (P<0.05). The migration and invasion abilities were inhibited (P<0.05). Conclusion To inhibit GGTase-Ⅰ expression, the migration and invasion abilities of tongue squa-mous cancer cells should also be inhibited. Further studies on GGTase-Ⅰ may provide novel effective molecular targets for tongue squamous cancer cells.

Objective This research aims to further explore the expression and significance of proliferating cell nuclear antigen (PCNA) and P53 in regenerating rat atrophy parotid gland from the gene and protein levels. Methods One hundred and two Wistar rats were randomly divided into experimental and control groups; the former group’s duct was ligated and then released respectively in 7 (Group A) and 14 days (Group B). Fresh parotid specimens were obtained at 0, 3, 5, 7, 10, 14, 21, and 28 days after being released. Hematoxylin-eosin staining method was used to observe the morphological changes of the parotid gland. The significance of P53 and PCNA in two groups was resolved by real-time fluorescence quantitative polymerase china reaction and Western blot. Results Acinar cells aoptosis and duct cells proliferation occurred when the occlusion of the parotid duct was reversed on days 7 and 14. The expression of P53 was higher than that of PCNA, and they reached the peak at the third and fifth days after groups A and B regenerated, respectively. This finding was significantly different compared with the control (P<0.01). P53 and PCNA contents decreased gradually; acinar and duct gradually returned to normal morphology; PCNA and P53 contents gradually close to the normal control group. Conclusion After ligating the parotid duct, P53 was highly expressed, and induced parotid gland atrophy. Mean-while, PCNA was highly expressed, which then decreased inducing gland recovery.

Objective To construct srtA-gene deletion mutant of Streptococcus mutans (S. mutans) UA159 with IFDC2 cassette through overlapping polymerase chain reaction (PCR) and allelic homologous recombination. Methods First, the upstream and downstream fragments surrounding the srtA and IFDC2 cassette were PCR amplified and ligated through over-lapping PCR. The resulting amplicon was transformed into UA159, and positive transformants were selected on BHI plates containing erythromycin. Second, upstream and downstream fragments of srtA with overlap regions were generated by PCR and were overlapped to create upΔ-down amplicon. Then, the upΔ-down amplicon was transformed into the aforementioned positive transformants and selected on BHI plates containing p-Cl-Phe. Results The PCR analysis and DNA sequencing results indicated that the coding region of the srtA was completely deleted, and the upstream and downstream regions flanking the srtA were ligated seamlessly. Conclusion The markerless srtA-deletion mutant of S. mutans was constructed successfully, which laid a foundation for further study of its biological function and influence on the biofilm formation of S. mutans.

Objective This study aimed at determining the influence of hydrofluoric acid (HF) in varied concentrations on the surface morphology of lithium disilicate glass ceramics and bond durability between resin composites and post-treated lithium disilicate glass ceramics. Methods After being sintered, ground, and washed, 72 as-prepared specimens of lithium disilicate glass ceramics with dimensions of 11 mm×13 mm×2 mm were randomly divided into three groups. Each group was treated with acid solution [32% phosphoric acid (PA) or 4% or 9.5% HF] for 20 s. Then, four acidified specimens from each group were randomly selected. One of the specimens was used to observe the surface morphology using scanning electron microscopy, and the others were used to observe the surface roughness using a surface roughness meter (including Ra, Rz, and Rmax). After treatment with different acid solutions in each group, 20 samples were further treated with silane coupling agent/resin adhesive/resin cement (Monobond S/Multilink Primer A&B/Multilink N), followed by bonding to a composite resin column (Filtek™ Z350) with a diameter of 3 mm. A total of 20 specimens in each group were randomly divided into two subgroups, which were used for measuring the microshear bond strength, with one of them subjected to cool-thermal cycle for 20 000 times. Results The surface roughness (Ra, Rz, and Rmax) of lithium disilicate glass ceramics treated with 4% or 9.5% HF was significantly higher than that of the ceramic treated with PA (P<0.05). The lithium disilicate glass ceramics treated with 9.5% HF also demonstrated better surface roughness (Rz and Rmax) than that of the ceramics treated with 4% HF. Cool-thermal cycle treatment reduced the bond strength of lithium disilicate glass ceramics in all groups (P<0.05). After cool-thermal cycle, the lithium disilicate glass ceramics treated with HF had higher bond strength than that of the ceramics treated with PA. The lithium disilicate glass ceramics treated with 4% HF had higher bond strength than that of the ceramics treated with 9.5% HF (P<0.05). During cool-thermal cycle, the lithium disilicate glass ceramics treated with 4% HF demonstrated higher reduction in bond strength than that of the samples treated with 9.5% HF (P<0.05). Conclusion The concentration of HF significantly affected the surface morphology of lithium disilicate glass ceramics and the bond durability between resin composites and post-treated lithium disilicate glass ceramics. The bond strength between resin composites and post-treated lithium disilicate glass ceramic was more efficiently maintained by treatment with 9.5% HF.

Objective To compare the effect of different surface processes on bond strength and microscopic structure using a scanning electron microscope (SEM) and an energy distribution spectrum (EDS) at the bonding interface between zirconia framework and veneering ceramic. Methods WIELAND zirconia core material was cut into 33 rectangular specimens and fired on into rectangular specimens (10 mm×5 mm×5 mm). The specimens were randomly divided into three groups (n=11). The sandblasting group was sandblasted before firing. The sandblasting and liner coverage group was sandblasted before firing and then sintered with liner coverage after firing. The control group was not processed. All the veneering ceramics (5 mm×5 mm×5 mm) were fired on into the zirconia substructure by slip-casting technique. One bilayered specimen in each group was prepared for SEM and EDS to examine the bonding conditions. The other specimens were measured for shear force using an electronic universal dynamometer. The data obtained were analyzed by using the statistical software SPSS 17.0. Results The values of the shear bond strength test were (13.80±1.54) MPa for the control group, (18.06±0.59) MPa for the sandblasting group, and (21.04±1.23) MPa for the sandblasting and liner coverage group. Significant differences existed among the three groups (P<0.01). Conclusion Abrasion before firing significantly increases the shear bond strength of zirconia to veneering porcelain. The use of porcelain combined with liner increases the shear bond strength.

Objective This study aimed at investigating and measuring the positional relationship between the long axis of the mandibular anterior teeth and the alveolar bone using cone beam computed tomography (CBCT) to provide reference data for implant treatment. Methods From the CBCT image database, 150 cases of radiographic data were selected according to the inclusion criteria and then were divided into six groups: males’ mandibular central incisors, males’ mandibular lateral incisors, males’ mandibular canines, females’ mandibular central incisors, females’ mandibular lateral incisors, and females’ mandibular canines. The angle (β) formed by the long axis of the mandibular anterior teeth and the corresponding alveolar bone was measured and recorded. Based on the long axis of alveolar bone, if the teeth incline to the lingual side, the value of the angle (β) was positive; otherwise, the value was negative. The resultant data were analyzed by SPSS 19.0. Results The β of the mandibular central incisors presented a mean value of 4.77° (range: -8.17°-16.10°) for male subjects and 5.38° (range: -5.63°-12.93°) for female subjects. The β of the mandibular lateral incisors exhibited a mean value of 6.12° (range: -2.87°-17.57°) for male subjects and 5.81° (range: -7.70°-15.70°) for female subjects. Finally, the β of the mandibular canines pre-sented a mean value of 5.01° (range: -8.13°-17.67°) for male subjects and 6.21° (range: -7.70°-17.87°) for female subjects. The percentages of the β between -10° and 10° of males’ mandibular incisors, mandibular lateral incisors, and mandibular canines were 87.34%, 80.67%, and 88.00%, respectively and those of females were 90.67%, 82.66%, and 82.66%, respectively. Whether male or female, the percentages of the β between -10° and 10° of the mandibular anterior teeth were more than 80%. The β that inclined to the lingual was not more than 20° and to the labial did not exceed 10°. Conclusion The long axis of the mandibular anterior teeth was almost consistent with the long axis of the alveolar bone. Therefore, the positional relationship could be referred to make reasonable implants treatment plan.

Objective This study used cone beam computed tomography (CBCT) to access the distribution and position of accessory mandibular foramina in the mandibular body of Jiangxi adults, and some safety rules of operation for clinics were provided. Methods Two hundred CBCT image subjects of Jiangxi adults were selected, which were divided into 4 groups by age with the same sex ratio. The number and position of accessory mandibular foramina in mandibular anterior teeth, premolars and molars region were described and the relationship between accessory mandibular foramina incidence and factors of age, gender and lateral were analyzed. Results A total of 1 123 accessory mandibular foramina were found, with a mean of 5.62±2.10 per person. The accessory mandibular foramina incidence decreased from the mesial region to the distal, and the lingual was higher than the buccal. There was no significant difference in the number of accessory mandibular foramina in male and female (P=0.195). However, the number of accessory mandibular foramina was negatively correlated with age (r_s=-0.301). Three highest frequency regions of accessory mandibular foramina were mandibular symphysis area (98.0%), lingual alveolar area of lower medial (88.0%) and lateral incisors and inferior area of lingual premolar (55.0%). Conclusion Accessory mandi-bular foramina are widely presented in the body of everyone’s mandible, addition attention should be paid to avoid the compli-cations causing by the damage of canal contents in the clinics.

Objective To study the effectiveness of dexmedetomidine used for general anesthesia maintenance in children undergoing odontotherapy in day-surgery operating room in reducing the incidence of emergence agitation (EA). Methods Eighty children undergoing odontotherapy and under general anesthesia in day-surgery operating room were randomized into two groups, group A (n=40) and group B (n=40). Each patient in group A was administered with a bolus dose of dexmedeto-midine (1.0 μg·kg^-1, saline diluted to 10 mL) pump-infused after intubation and a maintenance dose of 0.1-0.4 mL·(kg·h)^-1 followed-up until 45 min before the end of operation. Each patient in group B was administered with a bolus dose of normal saline 10 mL pump-infused after intubation and maintenance dose of 0.1-0.4 mL·(kg·h)^-1 followed-up until 45 min before theend of operation. Gender, age, weight, physical status according to the American Society of Anesthesiologists, perioperative heart rate (HR), mean arterial pressure (MAP), pulse oxygen saturation (SpO₂), sufentanil dosage, duration of surgery, time of extubation, time of regaining consciousness, and time to reach modified Aldrete’s score≥12 were recorded. Behavior in postanesthesia care unit was rated on the four-point agita-tion scale. Results Compared with group B, decreases were observed in HR and MAP at the beginning of operation, in 10 and 30 min, 1 and 2 h after the beginning of operation, and after extubation of group A (P<0.05). Sufentanil dosage and incidence of EA during recovery of group A were also lower than those of group B (P<0.05). Time to regain consciousness and time to reach modified Aldrete’s score≥12 of group A were longer than those of group B (P<0.05). No statistical difference was observed between other indexes of the two groups. Conclusion As an anesthetic used for general anesthesia maintenance in children undergoing odontotherapy in day-surgery operating room, dexmedetomidine results in low incidence of EA during recovery and more stable vital signs.

Objective Our research aimed to detect the efficacy of non-surgical periodontal treatment with Nd: YAG laser and scaling and root planning (SRP) for chronic periodontitis. Methods We recruited chronic periodontitis patients who have more than four teeth with clinical pocket depth of 4-8 mm. These teeth were distributed in four different zones within the oral cavity. Moreover, the teeth were single root teeth and not adjacent to each other. The subordinated teeth were randomized into four groups, as follows: no treatment (C group), simple SRP (SRP group), Nd: YAG laser after SRP treatment (SRP+L group), and SRP after Nd: YAG laser treatment (L+SRP group). The four experimental observation points were as follows: before treatment (baseline) and 1 week, 1 month, and 3 months after treatment. We measured clinical indicators and collected subgingival deposits in the four time points to analyze changes of red complex in periodontitis. Results The clinical indi-cators were better in all treatment groups than in the control group. Comparison among treatment groups indicated that the value of bleeding on probing, periodontal probing depth, and clinical attachment loss showed no difference. However, the value of plaque index in SRP+L and L+SRP presented a significant reduction at 3 months after treatment. The percentages of Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola in all treatment groups decreased after clinical treatment, and differences were observed among the treatment groups at different time points. Conclusion Non-surgical periodontal treatment with SRP and Nd: YAG laser is not more effective than SRP monotherapy. The sequence of laser treatment and SRP has no significant effect on the treatment. However, SRP with Nd: YAG laser was beneficial for plaque control. Non-surgical periodontal treatment with Nd: YAG laser may be used as an alternative to reduce and control the proliferation of microorganisms in persistent periodontitis, but it still needs further verification.

Objective This study aims to investigate the characteristics and general rules of adverse drug reactions (ADR) in a 3A-grade stomatology hospital for safe and rational drug use in clinical stomatology. Methods We retrospectively ana-lyzed 52 ADR cases (1:1.36, males:females) reported in the West China Hospital of Stomatology of Sichuan University from 2014 to 2016 in terms of gender and age distributions, drug categories, and clinical manifestations. Results Eight kinds of drugs and antibiotics were predominately used [24 cases, 46.15% (24/52)], followed by nutrition drugs and antitumor drugs. Cephalosporin was the leading antibiotic drug associated with ADR [20 cases, 83.33% (20/24)]. Intravenous infusion was the most common route of drug administration [49 cases, 94.23% (49/52)]. The most common manifestations of the ADR were damages of the skin and its appendages and lesions of the digestive and nervous systems. Conclusion Streng-thening the ADR monitoring system and further management should be implemented to alleviate ADR in stomatology hospitals.

Objective This study aims to assess the remineralization effect of casein phosphopeptide (CPP)-amorphous calcium phosphate (ACP) on enamel demineralization by performing system review of randomized controlled trials (RCT) involving the treatment of enamel demineralization with CPP-ACP. Methods The study was developed based on the Cochrane handbook for systematic reviews of interventions (Version 5.1.0) and included the following: search strategy, selection criteria, data extraction, and risk of bias assessment. We searched electronic databases such as PubMed, Embase, Cochrane Library, CNKI, Wanfang and VIP up to September 2016. RCT of treating enamel demineralization with CPP-ACP were included. Data extraction and domain-based risk of bias assessment were independently performed by two reviewers. Results Twelve RCTs were included. Because of the difference of experimental design and evaluation standards, the quantitative analysis can not be carried out. Conclusion There is no strong evidence that CPP-ACP is superior to con-ventional fluoride formulations in enamel remineralization. However, due to the limitations of sample size, follow-up time and study design, more high quality and large-sample RCT are needed to further verify the evidence.

Objective This review aims to systematically evaluate the effect of decalcified freeze-dried bone allograft (DFDBA) combined with rich platelet derivatives on the treatment of human periodontal intrabony defects. Methods A search in PubMed, Web of Science, Embase, Cochrane Library, CNKI, and other electronic databases was conducted to iden-tify randomized controlled trials (RCT) of the use of DFDBA combined with rich platelet derivatives in the treatment of human periodontal intrabony defects, performed before May 2016. The quality of the RCTs was assessed.RevMan 5.3 software was applied for Meta-analysis. Results A total of nine RCTs were included. A total of 194 patients and 303 defects were involved. Short-term (6 months) and long-term (12 to 18 months) groups were included. Meta-analysis results revealed that DFDBA combined with rich platelet derivatives was superior to DFDBA or rich platelet derivatives alone for probing depth reduction in the short-term [MD=0.75 mm, 95% confidence intervals (CI) (0.31 mm, 1.20 mm), P=0.001 0] and long-term groups [MD=0.87 mm, 95%CI (0.02 mm, 1.72 mm), P=0.04], clinical attachment level gain in the short-term [MD=0.65 mm, 95%CI (0.08 mm, 1.22 mm), P=0.03] and long-term groups [MD=1.31 mm, 95%CI (0.60 mm, 2.01 mm), P<0.000 3], gingival recession reduction in the long-term group [MD=-0.58 mm, 95%CI (-0.78 mm, -0.38mm), P<0.000 01], bone fill gain in the short-term [MD=0.52 mm, 95%CI (0.03 mm, 1.00 mm), P=0.04] and long-term groups [MD=1.26 mm, 95%CI (0.65 mm, 1.86 mm), P<0.000 1]. Conclusion DFDBA combined with platelet rich derivatives is probably effective in the treatment of human periodontal intrabony defects. It is probably superior to DFDBA or platelet rich derivatives alone. Consi-dering the limitation of the included studies, high-quality and large-sample RCTs are required to evaluate the effect.

Condyle is a critical growth region of the mandible where mandible by endochondral ossification occurs. Con-dylar cartilage belongs to the secondary cartilage, which is not only affected by genetic factors but also by stress, drug intake, and other local factors. To promote the growth of the mandible, various exogenous and local factors were used to alter the biological environment of the condylar cartilage to stimulate endochondral ossification. This article reviews studies on the influence of exogenous factors on condylar growth and reconstruction. This literature review will provide a reference point for the treatment of patients with mandibular retraction.

Increasing scientific evidence supports the positive relationship between inflammation and cancer development. The immune response initiated by pattern recognition receptors is critical to triggering of tumor-associated inflammation. Triggering receptor expressed on myeloid cells (TREM) is an immunoglobulin of the super transmembrane glycoprotein family, which is mainly expressed on select groups of myeloid cells. The most important members of TREM comprise TREM-1 and TREM-2. Activation of TREM-1 and TREM-2 signaling is initiated upon binding of their ligands. Subsequently, cross-linking reactions of downstream effectors occur, resulting in inflammation regulation. Recently, the connection between TREM and malignant tumors has been widely noticed and studied. This review summarizes studies of association between TREM-1, TREM-2, and malignant tumors in the medical field to provide new ideas for study on the correlation between periodontitis and oral cavity cancer.

Autophagy is an intracellular conservative degradation pathway. This event has been considered as a key step in host defense against bacterial infection. However, Porphyromonas gingivalis, as one of the evidence-sufficient periodontal pathogens, can utilize self-induced autophagy to achieve persistent intracellular survival and proliferation, which enable this organism to escape from host immune surveillance. This review focuses on molecular mechanism of P. gingivalis internali-zation and autophagy to illuminate its pathogenesis and to further explore the relationship between P. gingivalis and systemic diseases.

With the development of microvascular technology, perforator flaps have gradually become a good alternative for reconstruction of tissue defects. However, the major limitations of perforator flaps include uncertainty in predicting ana-tomical location of perforators and high variability in perforator size and course, which require preoperative localization tech-niques. Recently, as one of the preoperative localization techniques, computed tomography angiography was used to determine the number, size, course, and exact emerging point of perforator flaps. Clinicians can reduce surgical complications and shorten operative time by using computed tomography angiography. However, only several studies reported clinical applications of computed tomography angiography. This article reviews characteristics, classification, and preoperative location techniques for perforator flaps and its problems.

This article reported jaw osteomyelitis in ancient human bone specimens, helping us to understand the emer-gence of ancient human jaw osteomyelitis and other oral health conditions.

Replacement resorption is the most frequent complication after delayed replantation of avulsed teeth. The resorption can interfere with the development of the alveolar ridge and lead to tilt of the adjacent teeth in growing patients. However, there is no means of arresting or reversing the process. Recently decoronation is recommended by International Assocaition of Dental Traumatology as the optimal choice to manage it. This paper demonstrates the procedure and effecti-veness of the decoronation by literature review and a case report with 4-year follow-up.