Abstract:

Objective　To investigate the effect of anti-ICAM-1/LAF-1 McAb on the cytotoxicity of tumor infiltrating lym- phocytes from oral squamous cell carcinoma.Methods　TILwere isolated from fresh tumor tissues in 15 patients with oral squa- mous cell carcinoma and culturedin vitro. The expression of ICAM-1/LAF-1 in fresh and activated TILwas examined by FACS. The cytotoxicity of TIL against auto tumor cells and the level ofTNF-αproduced byTILwere compared before and after interrupted with anti- ICAM-1/LAF-1 McAb.Results　The expression of ICAM-1and LAF-1 in the activatedTIL significantly increased com- paring with freshTIL (P<0·05). Interrupted by anti-ICAM-1 McAb, the cytotoxicity ofTIL against auto tumor cell was not sig- nificantly changed. Interrupted by anti-ICAM-1 and anti-LAF-1 McAb, the level ofTNF-αproduced byTIL showed no differences comparing with the control (P<0·05), but the cytotoxicity of TIL against auto tumor cells significantly decreased (P<0·05). Conclusion　The effect of anti-LAF-1 McAb may result from steric interference of antigen-nonspecific adhesion process.

Key words: carcinoma, squamous cell, T-lymphocyte, cytotoxicity, adhesive molecular