West China Journal of Stomatology

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An Experimental Study on Gene Transfection of Human Interleukin-1 Receptor Antagonist Gene into Chondrocytes of Temporomandibular Joint

LI Yisong,TIANWeidong,WANG Dong,et al.   

  1. Department ofOral andMaxillofacial Surgery,West China College ofStomatolo- gy,Sichuan University,Chengdu610041China
  • Received:2003-02-25 Revised:2003-02-25 Online:2003-02-20 Published:2003-02-20

Abstract:

Objective The purpose of this study was to investigate methods and expression of the recombinant human inter- leukin-1 receptor antagonist gene transfected into chondrocytes of temporomandibular joints (TMJ).Methods Chondrocytes derived from the TMJ of 5~7 months old human fetus were transfected by recombinant human interleukin-1 receptor antagonist gene via cat- ionic liposome (Lipefectine) as a medium. The expressing level of hIL-1ra protein was detected using immunocytochemistry and enz- yme-linked immunosorbent assay (ELISA).Results The positive results of the immunocytochemistry were found. The positive ex- pression was detected in the cell plasma and the cell culture supernatant, and there was significant difference between the cells with and without gene transfection (P<0.01).Conclusion This feasible method provides experimented evidence for the future gene therapy of temporomandibular joint osteoarthrosis.

Key words: chondrocyte, human interleukin-1 receptor antagonist, temporomandibular joint, osteoarthrosis, gene transfection