West China Journal of Stomatology ›› 2020, Vol. 38 ›› Issue (5): 495-501.doi: 10.7518/hxkq.2020.05.004
Previous Articles Next Articles
Liu Jianan1(), Ma Zhaolei1, Su Rongjian2, Huang Keqiang1(
)
Received:
2019-12-31
Revised:
2020-07-16
Online:
2020-10-01
Published:
2020-10-14
Contact:
Huang Keqiang
E-mail:liujianan828@163.com;hkq9@163.com
Supported by:
CLC Number:
Liu Jianan, Ma Zhaolei, Su Rongjian, Huang Keqiang. Effect of enhancer of zeste homolog 2 inhibitor GSK126 on the proliferation and apoptosis of tongue squamous cell carcinoma[J]. West China Journal of Stomatology, 2020, 38(5): 495-501.
Tab 1
Relative expression levels of each protein at different concentrations of GSK126
浓度/(μmol·L-1) | ERK | p-ERK | Bax | Bcl-2 | Cleaved caspase-9 |
---|---|---|---|---|---|
0 | 1.842±0.076 | 1.566±0.103 | 0.989±0.004 | 1.359±0.048 | 0.803±0.020 |
1 | 1.873±0.127 | 1.332±0.068 | 1.084±0.008 | 1.037±0.055 | 0.843±0.031 |
2.5 | 1.742±0.084 | 1.313±0.028 | 1.102±0.044 | 1.020±0.043 | 0.889±0.056 |
5 | 1.777±0.037 | 1.076±0.030 | 1.113±0.049 | 0.924±0.082 | 0.907±0.058 |
10 | 1.724±0.031 | 1.071±0.062 | 1.146±0.050 | 0.906±0.072 | 1.015±0.035 |
15 | 1.728±0.055 | 1.052±0.035 | 1.242±0.030 | 0.847±0.095 | 1.038±0.010 |
F值 | 2.075 | 34.431 | 15.735 | 21.346 | 17.065 |
P值 | 0.139 | 0.000 | 0.000 | 0.000 | 0.000 |
[1] |
Ferlay J, Colombet M, Soerjomataram I , et al. Estimating the global cancer incidence and mortality in 2018: GLOBOCAN sources and methods[J]. Int J Cancer, 2019,144(8):1941-1953.
doi: 10.1002/ijc.31937 URL pmid: 30350310 |
[2] |
Hussein AA, Helder MN, de Visscher JG, et al. Global incidence of oral and oropharynx cancer in patients younger than 45 years versus older patients: a systematic review[J]. Eur J Cancer, 2017,82:115-127.
doi: 10.1016/j.ejca.2017.05.026 URL pmid: 28654785 |
[3] |
De Paz D, Kao HK, Huang Y , et al. Prognostic stratification of patients with advanced oral cavity squamous cell carcinoma[J]. Curr Oncol Rep, 2017,19(10):65.
doi: 10.1007/s11912-017-0624-3 URL pmid: 28799122 |
[4] |
Kim KH, Roberts CW . Targeting EZH2 in cancer[J]. Nat Med, 2016,22(2):128-134.
doi: 10.1038/nm.4036 URL pmid: 26845405 |
[5] | Li Z, Wang Y, Qiu J , et al. The polycomb group protein EZH2 is a novel therapeutic target in tongue cancer[J]. Oncotarget, 2013,4(12):2532-2549. |
[6] |
Shen L, Cui J, Liang SM , et al. Update of research on the role of EZH2 in cancer progression[J]. Onco Targets Ther, 2013,6:321-324.
doi: 10.2147/OTT.S42453 URL pmid: 23589697 |
[7] |
Huang X, Yan J, Zhang M , et al. Targeting epigenetic crosstalk as a therapeutic strategy for EZH2-aberrant solid tumors[J]. Cell, 2018,175(1):186-199.
doi: 10.1016/j.cell.2018.08.058 URL pmid: 30220457 |
[8] |
Almangush A, Coletta RD, Bello IO , et al. A simple novel prognostic model for early stage oral tongue cancer[J]. Int J Oral Maxillofac Surg, 2015,44(2):143-150.
doi: 10.1016/j.ijom.2014.10.004 URL pmid: 25457829 |
[9] |
Tang QM, Cheng B, Xie MR , et al. Circadian clock gene bmal1 inhibits tumorigenesis and increases paclitaxel sensitivity in tongue squamous cell carcinoma[J]. Cancer Res, 2017,77(2):532-544.
doi: 10.1158/0008-5472.CAN-16-1322 URL pmid: 27821487 |
[10] |
Zeng DL, Liu MX, Pan JX . Blocking EZH2 methylation transferase activity by GSK126 decreases stem cell-like myeloma cells[J]. Oncotarget, 2017,8(2):3396-3411.
doi: 10.18632/oncotarget.13773 URL pmid: 27926488 |
[11] |
Wu C, Jin X, Yang J , et al. Inhibition of EZH2 by chemo- and radiotherapy agents and small molecule inhibitors induces cell death in castration-resistant prostate cancer[J]. Oncotarget, 2016,7(3):3440-3452.
doi: 10.18632/oncotarget.6497 URL pmid: 26657505 |
[12] |
Gulati N, Béguelin W, Giulino-Roth L . Enhancer of zeste homolog 2 (EZH2) inhibitors[J]. Leuk Lymphoma, 2018,59(7):1574-1585.
doi: 10.1080/10428194.2018.1430795 URL pmid: 29473431 |
[13] |
Koppens MA, Bounova G, Cornelissen-Steijger P , et al. Large variety in a panel of human colon cancer organoids in response to EZH2 inhibition[J]. Oncotarget, 2016,7(43):69816-69828.
doi: 10.18632/oncotarget.12002 URL pmid: 27634879 |
[14] |
Deschênes-Simard X, Gaumont-Leclerc MF, Bourdeau V , et al. Tumor suppressor activity of the ERK/MAPK pathway by promoting selective protein degradation[J]. Genes Dev, 2013,27(8):900-915.
doi: 10.1101/gad.203984.112 URL pmid: 23599344 |
[15] |
Kidger AM, Keyse SM . The regulation of oncogenic Ras/ERK signalling by dual-specificity mitogen activated protein kinase phosphatases(MKPs)[J]. Semin Cell Dev Biol, 2016,50:125-132.
doi: 10.1016/j.semcdb.2016.01.009 URL pmid: 26791049 |
[16] |
Chan LP, Liu C, Chiang FY , et al. IL-8 promotes inflammatory mediators and stimulates activation of p38 MAPK/ERK-NF-κB pathway and reduction of JNK in HNSCC[J]. Oncotarget, 2017,8(34):56375-56388.
doi: 10.18632/oncotarget.16914 URL pmid: 28915597 |
[17] |
Xu XB, Lai YY, Hua ZC. Apoptosis and apoptotic body: disease message and therapeutic target potentials[J]. Biosci Rep, 2019, 39(1): BSR20180992.
doi: 10.1042/BSR20180789 URL pmid: 30606744 |
[18] |
Bonneau B, Ando H, Kawaai K , et al. IRBIT controls apoptosis by interacting with the Bcl-2 homolog, Bcl2l10, and by promoting ER-mitochondria contact[J]. Elife, 2016,5:e19896.
doi: 10.7554/eLife.19896 URL pmid: 27995898 |
[19] |
Maes ME, Schlamp CL, Nickells RW . Live-cell imaging to measure BAX recruitment kinetics to mitochondria during apoptosis[J]. PLoS One, 2017,12(9):e0184434.
doi: 10.1371/journal.pone.0184434 URL pmid: 28880942 |
[20] |
Li P, Zhou LB, Zhao T , et al. Caspase-9: structure, mechanisms and clinical application[J]. Oncotarget, 2017,8(14):23996-24008.
doi: 10.18632/oncotarget.15098 URL pmid: 28177918 |
[21] |
Maes ME, Schlamp CL, Nickells RW . BAX to basics: how the BCL2 gene family controls the death of retinal ganglion cells[J]. Prog Retin Eye Res, 2017,57:1-25.
doi: 10.1016/j.preteyeres.2017.01.002 URL pmid: 28064040 |
[1] | Sun Qiaozhen, Shi Fan, Luo Dan, Xu Ting, Wang Shengzhi. Thermo-chemotherapy enhances the immunogenicity of oral squamous cell carcinoma cells by inducing the expression of damage-associated molecular patterns [J]. West China Journal of Stomatology, 2020, 38(5): 502-508. |
[2] | Sun Xuehui, Fan Xin, Hu Kaili, Hu Wenting. Effects of RAB1A on the proliferation, invasion, and metastasis of tongue squamous cell carcinoma cells [J]. West China Journal of Stomatology, 2020, 38(3): 245-249. |
[3] | Sheng Shangui, Wang Ya’nan, Wang Shaoru, Zhao Kai, Wang Yunying, Xu Xiaona, Wang Qimin, Tong Lei, Chen Zhenggang. Effects of farnesyltransferase silencing on the migration and invasion of tongue squamous cell carcinoma [J]. West China Journal of Stomatology, 2020, 38(2): 177-184. |
[4] | Zheng Jing, Zhang Yuwen, Li Tianke, Bao Yang, Zhang Suxin. Effect of miR-204-5p on the proliferation, migration, and invasion on tongue squamous cell carcinoma SCC25 cells by targeting bromodomain-containing protein 4 [J]. West China Journal of Stomatology, 2020, 38(2): 185-192. |
[5] | Wang Jian,Yan Guangpeng,Guo Chao,Li Jun. Expression and significance of microRNA-125b in tongue squamous cell carcinoma [J]. West China Journal of Stomatology, 2020, 38(1): 11-16. |
[6] | Wang Liwei,Yu Yu,Chen Jiao,Feng Yun,Cui Bomiao,Li Xiaoying,Wang Jingnan,Chen Hongli,Zhang Ping. Protein kinase D1 regulates the growth and metabolism of oral squamous carcinoma cells in tumor microenvironment [J]. West China Journal of Stomatology, 2019, 37(6): 577-582. |
[7] | Geng Yudong,Wang Shubin,Lu Taiqing,Teng Wei. Expression and functions of long non-coding RNA actin filament-associated protein 1-antisense RNA1 in oral squamous cell carcinoma [J]. West China Journal of Stomatology, 2019, 37(6): 594-601. |
[8] | Su Wen,Wang Yufan,Wang Feng,Yang Huijun,Yang Hongyu. Effect of circular RNA hsa_circ_0002203 on the proliferation, migration, invasion, and apoptosis of oral squamous cell carcinoma cells [J]. West China Journal of Stomatology, 2019, 37(5): 509-515. |
[9] | Jiehang Li,Zhifei Su,Xuan Bai,He Yuan,Jiyao Li. Effect of zoladronate on the proliferation and osteogenic differentiation of rat bone mesenchymal stem cells [J]. West China Journal of Stomatology, 2019, 37(3): 242-247. |
[10] | Zengwen Yue,Shubin Wang,Jinzhong Liu. Effects of large tumor suppressor homolog 2 gene overexpression on the proliferation and apoptosis of oral squamous cell carcinoma [J]. West China Journal of Stomatology, 2018, 36(6): 609-612. |
[11] | Zheng Fang, Feng Qiu, Junfang Zhao, Qiang Sun, Bin Qiao, Guanghui Li, Xinming Li. Role and mechanism of Galectin-3 gene in proliferation, invasion, and apoptosis of oral squamous cell carcinoma [J]. West China Journal of Stomatology, 2018, 36(4): 404-409. |
[12] | Jun Huan, Lei Dou, Qifang Yan, Deqin Yang. An in vitro study of the angiogenic effects of concentrate growth factor on human umbilical vein endothelial cells [J]. West China Journal of Stomatology, 2018, 36(3): 247-251. |
[13] | Xuefeng Gao, Haibin Jiao, Changcheng Ye, Yingqun. Liu. Effects of protein kinase C and motigen-activated protein kinase kinase/extracellular regulated protein kinases signaling pathway on mRNA level of inducible nitric oxide synthase in Tca8113 cells [J]. West China Journal of Stomatology, 2018, 36(2): 133-139. |
[14] | Huaying Shao, Yigong Zhang, Xue Yang, Qiongyue Zhang, Xiaohong Wu. Effects of inhibitory concentration minocycline on the proliferation, differentiation, and mineralization of osteoblasts [J]. West China Journal of Stomatology, 2018, 36(2): 140-145. |
[15] | Jin Qiu, Huiyu Zhang, Li Liu, Yinghui Tan. Effect of bone morphogenetic protein-4 overexpression on the biological activity of mouse induced pluripotent stem cells [J]. West China Journal of Stomatology, 2018, 36(2): 190-193. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||