West China Journal of Stomatology ›› 2020, Vol. 38 ›› Issue (2): 139-144.doi: 10.7518/hxkq.2020.02.005

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Biology behavior of head and neck squamous cell cancer cells changes after knocking down heat shock protein 27

Zhu Zhenkun, Wang Yushang, Xu Xin()   

  1. Dept. of Implantology, School and Hospital of Stomatology, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan 250012, China
  • Received:2019-08-12 Revised:2020-01-13 Online:2020-04-01 Published:2020-04-15
  • Contact: Xin Xu E-mail:xinxu@sdu.edu.cn
  • Supported by:
    Shandong Taishan Scholar Project Special Fund(ts201511106);Interdisciplinary Program of Shandong University(2016JC024)

Abstract:

Objective This study aimed to observe the metastatic behavior of head and neck squamous cell carcinoma cells after knocking down heat shock protein (Hsp) 27. Methods The experiment was divided into three groups: the lentivirus vector plasmid of pLenti-shRNA-Hsp27 was transfected into UM-SCC-22B cells as experimental group (shHsp27 group), routine culture of UM-SCC-22B cells as blank control (ctrl group), UM-SCC-22B cells transfection of pLenti-shRNA-ctrl lentivirus vector as negative control (shctrl group). Through real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot assay to detect the mRNA expression of Hsp27 in three groups. MTS assay was performed to detect cell-proliferation changes, wounding healing assay was performed to detect cell-migration changes, and Matrigel Transwell invasion assay was performed to detect cell-invasion changes. Results The expression of Hsp27 in shHsp27 group decreased significantly; MTS assay showed that UM-SCC-22B before and after Hsp27 knockdown had similar proliferation rates after being cultured for 24 or 48 h. Compared with the ctrl group, the shHsp27 group decreased the metastatic behavior by 4.38-fold in migration and 2.03-fold in cell invasion. Conclusion Stably transfected lentivirus vector plasmid of pLenti-shRNA-Hsp27 can efficiently decrease Hsp27 expression and reduce the metastasis ability of UM-SCC-22B.

Key words: heat shock protein 27, human head and neck squamous cell cancer, metastasis, lentivirus transfection

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