West China Journal of Stomatology

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The effects of oxymatrine on expression of interleukin-6 and interleukin-1β mRNA of human periodontal ligament cell stimulated by lipopolysaccharides

WU Yun1, CHEN Ling1, LUO Kai2, YAN FU-hua2   

  1. 1. Dept. of Stomatology, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, China;2. Dept. of Periodontology,School of Stomatology, Fujian Medical University, Fuzhou 350002, China
  • Received:2010-12-25 Revised:2010-12-25 Online:2010-12-20 Published:2010-12-20
  • Contact: LUO Kai,Tel:0591-83736427


Objective To observe the effects of oxymatrine on the expression of interleukin-6(IL-6), interleukin- 1β(IL-1β)mRNA of human periodontal ligament cell(PDLC) stimulated by lipopolysaccharides(LPS), and to discuss oxymatrine’s inhibition mechanism on periodontal inflammation stimulated by LPS. Methods Firstly, isolate PDLC externally and culture them; for oxymatrine experimental group we used different combination of LPS and oxymatrine in different concentration, and for the matched group we use DEME nutrient solutions of 1% FBS. Then reverse transcription -polymerase chain reaction(RT -PCR) for checking the level of IL -6 and IL -1β mRNA. Results 25 μg·mL-1 LPS can significantly enhance the expression of both IL-6 and IL-1β mRNA’s level, and oxymatrine could restrain above phenomena. Conclusion Oxymatrine can restrain the expression of IL-6 and IL-1β mRNA of human PDLC stimulated by LPS.

Key words: oxymatrine, lipopolysaccharides, periodontal ligament cell, inflammation