West China Journal of Stomatology

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The experimental study on porous calcium phosphate cement with bone marrow stromal cells for bone tissue engineering

WANG Lei1, LI Yao -jun2, ZHANG Yan3, PAN Ke -feng3, HUANG Yuan -liang2, LIU Chang -sheng4, JIANG Xin-quan5   

  1. 1. Dept. of Stomatology, Hongqiao Community Health Center, Shanghai 200051, China; 2. Dept. of Stomatology, East Hospital, Tongji University, Shanghai 200120, China; 3. Dept. of Oral and Maxillofacial Surgery, School of Stomatology, Tongji University, Shanghai 200072, China; 4. School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China; 5. Laboratory of Bioengineering, The Ninth People ′s Hospital, Medical College, Shanghai Jiaotong University, Shanghai 200011, China
  • Received:2010-06-25 Revised:2010-06-25 Online:2010-06-20 Published:2010-06-20
  • Contact: HUANG Yuan -liang,Tel:021-38804518-1156


Objective To observe the biocompatibility of new biomaterials porous calcium phosphate(CPC) and ectopic bone formation of CPC with bone marrow stromal cells(BMSCs). Methods The BMSCs were cultured from Beagle dog and combined with the porous CPC with the best concentration after transfect green fluorescent protein (GFP). The adhesion and growth of BMSCs on CPC were observed under inversion, fluorescence and scanning electron microscopy. The ectopic bone formation were observed at the 8th week after CPC and BMSCs were implanted subcutaneously into nude mice. Results When BMSCs with CPC were cultured at the 1st day, cells were climbing out from CPC with normal morphology. At the 7th day cells can be seen protruding pseudopods, secretion of matrix. Bone formation could be seen histomorphologically at the 8th week. Conclusion Porous CPC has good biocompatibility and is an ideal scaffold material for bone tissue engineering.

Key words: porous calcium phosphate, bone tissue engineering, scaffold material, bone marrow stromal cells