口腔黏膜癌前病变和口腔癌动物模型的研究进展

doi:10.7518/hxkq.2020.02.015

华西口腔医学杂志 ›› 2020, Vol. 38 ›› Issue (2): 198-204.doi: 10.7518/hxkq.2020.02.015

口腔黏膜癌前病变和口腔癌动物模型的研究进展

程俊鑫, 白贺天, 常治楠, 李敬(), 陈谦明

口腔疾病研究国家重点实验室国家口腔疾病临床医学研究中心四川大学华西口腔医院,成都 610041

收稿日期:2019-07-12 修回日期:2020-01-07 出版日期:2020-04-01 发布日期:2020-04-15
通讯作者: 李敬 E-mail:lijing1984@scu.edu.cn
作者简介:程俊鑫,学士,E-mail：jxcheng@stu.scu.edu.cn
基金资助:
国家重点研发计划重点专项课题(2017YFC0840100);国家重点研发计划重点专项课题(2017YFC0840107);国家自然科学基金(81872211);国家自然科学基金(81672675);国家自然科学基金(81621062);高等学校学科创新引智计划（111计划）(B14038);四川大学大学生创新创意实验项目(20171082);四川大学大学生创新创意实验项目(20171083)

Development of precancerous lesions of oral mucous membrane diseases and oral cancer animal models

Cheng Junxin, Bai Hetian, Chang Zhinan, Li Jing(), Chen Qianming

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China

Received:2019-07-12 Revised:2020-01-07 Online:2020-04-01 Published:2020-04-15
Contact: Jing Li E-mail:lijing1984@scu.edu.cn
Supported by:
National Key Research and Development Plan of China(2017YFC0840100);National Key Research and Development Plan of China(2017YFC0840107);The National Natural Science Foundation of China(81872211);The National Natural Science Foundation of China(81672675);The National Natural Science Foundation of China(81621062);Overseas Expertise Introduction Project for Discipline Innovation(111) Project(B14038);Creative Innovation Experiment Project of College Students of Sichuan University(20171082);Creative Innovation Experiment Project of College Students of Sichuan University(20171083)

摘要/Abstract

摘要：

口腔黏膜癌前病变和口腔癌是发展中国家的常见疾病尤其是在南亚地区,其癌变机制尚不明确,目前缺乏有效防治措施,因此口腔黏膜癌前病变及口腔癌动物模型建立显得尤为重要。本文结合相关研究,就近年来口腔黏膜癌前病变及口腔癌动物模型建立方法的进展作一综述。

关键词: 口腔黏膜癌前病变, 口腔癌, 动物模型

Abstract:

Precancerous lesions of oral mucous membrane diseases and oral cancer are common diseases in developing countries, especially in South Asia. However, the cancerous mechanism remains unclear, and no efficient treatment and prognosis measure are currently available. Thus, precancerous lesions of the oral mucosa and oral cancer animal models must be identified to further understand their function. In this paper, we systematically review the development of oral mucosal precancerous lesions and oral cancer animal models by referring to related research.

Key words: precancerous lesions of oral mucous membrane disease, oral cancer, animal model

中图分类号:

程俊鑫, 白贺天, 常治楠, 李敬, 陈谦明. 口腔黏膜癌前病变和口腔癌动物模型的研究进展[J]. 华西口腔医学杂志, 2020, 38(2): 198-204.

Cheng Junxin, Bai Hetian, Chang Zhinan, Li Jing, Chen Qianming. Development of precancerous lesions of oral mucous membrane diseases and oral cancer animal models[J]. West China Journal of Stomatology, 2020, 38(2): 198-204.

图/表 2

表1

表2

参考文献 55

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主要方法	动物对象	处理方法	建模周期	建模成功率/%	模型解剖及组织生理与人类相似	操作接种简便	稳定性	癌变发展观察	参考文献
化学诱导	5周龄金黄地鼠	用0.005 g·L^-1的BaP/DMBA涂抹地鼠颊囊	15周	-	×	√	√	√	[13]
	6周龄小鼠	用0.000 1 g·L^-1的4NQO采取饮药法	24周	68.6	√	√	×	-	[14]
	6周龄小鼠	取0.000 5 g·L^-1的4NQO涂抹小鼠舌部	20周	97.1	√	√	√	√	[14]
慢性刺激+ 化学诱导	8周龄小鼠	采用慢性机械刺激及50%香烟烟丝丙酮提取液涂擦已拔髓针轻刮8周龄的小鼠舌左侧缘及左颊部中份黏膜处数次至发红或出现少许出血点	15~25周	-	×	√	√	√	[15]
原位移植	SCID的5~ 8周龄小鼠	向小鼠舌腹位置原位移植人非白色念珠菌感染的OLK病损组织	8周	81.2	√	×	√	√	[16]

处理方法	实验对象	效果简述	建模成功	参考文献
向大鼠的皮下注射氯化汞水溶液,导致其出现自身性免疫综合征,后局部涂抹氯化汞溶液	具有遗传易感性的棕色挪威鼠	8~10周后显示有单一症状的苔藓样病变	√	[19]
将人类非糜烂型OLP病损移植到小鼠的舌腹以及背部	SCID且NK细胞活性不强的小鼠	1周出现扁平苔藓病变;2周病理特征消失	×	[20]
对于口腔黏膜上皮与背部皮肤的表面采用针刺划痕法	金黄地鼠颊囊与背部皮肤	2周发现皮肤深处血管增生,炎细胞浸润; 4周发现大量炎细胞浸润,但维持时间短	×	[22]
将典型的扁平苔藓病灶移植到小鼠的皮肤上	裸鼠	扁平苔藓在6周之后病理特征基本消失和正常皮肤相似,但角质层在6周后仍然是角质化。	×	[21]

口腔黏膜癌前病变和口腔癌动物模型的研究进展

Development of precancerous lesions of oral mucous membrane diseases and oral cancer animal models

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