黏着斑激酶抑制剂TAE226对人口腔鳞状细胞癌细胞上皮间质转化的影响

doi:10.7518/hxkq.2020.01.004

华西口腔医学杂志 ›› 2020, Vol. 38 ›› Issue (1): 17-22.doi: 10.7518/hxkq.2020.01.004

黏着斑激酶抑制剂TAE226对人口腔鳞状细胞癌细胞上皮间质转化的影响

邹湘渝^1,²,曾琴^1,²,刘萍³,聂敏海^1,²()

1. 西南医科大学附属口腔医院牙周黏膜科,泸州 646000
2. 西南医科大学口颌面修复重建与再生实验室,泸州 646000
3. 深圳市罗湖区人民医院口腔科,深圳 518000

收稿日期:2019-03-21 修回日期:2019-08-19 出版日期:2020-02-01 发布日期:2020-02-06
通讯作者: 聂敏海 E-mail:517094363@qq.com
作者简介:邹湘渝,硕士,E-mail： 1456300692@qq.com
基金资助:
四川省科技计划(2018JY0401)

Effect of the focal adhesion kinase inhibitor TAE226 on the epithelial-mesenchymal transition in human oral squamous cell carcinoma cell line

Zou Xiangyu^1,²,Zeng Qin^1,²,Liu Ping³,Nie Minhai^1,²()

1. Dept. of Periodontics and Oral Medicine, The Affiliated Hospital of Stomatology, Southwest Medical University, Luzhou 646000, China
2. Laboratory for Reconstraction and Regeneration of Oral and Maxillofacial Region, Southwest Medical University, Luzhou 646000, China
3. Dept. of Stomatology, Luohu People’s Hospital of Shenzhen, Shenzhen 518000, China

Received:2019-03-21 Revised:2019-08-19 Online:2020-02-01 Published:2020-02-06
Contact: Minhai Nie E-mail:517094363@qq.com
Supported by:
Sichuan Science and Technology Program(2018JY0401)

摘要/Abstract

摘要：

目的探究黏着斑激酶抑制剂TAE226对人口腔鳞状细胞癌细胞上皮间质转化（EMT）过程的影响。方法不同浓度（0、1、5、10 μmol·L^-1）的TAE226作用于人口腔鳞状细胞癌HSC-3和HSC-4细胞24、48、72 h后,实时定量聚合酶链反应检测EMT标志物E-钙黏蛋白（E-cadherin）、波形蛋白（Vimentin）的mRNA表达;蛋白质印迹法检测E-cadherin、Vimentin在TAE226作用48 h后的蛋白表达。结果实时定量聚合酶链反应检测表明,随着TAE226作用时间和浓度的增加,E-cadherin mRNA的表达增加,Vimentin mRNA的表达降低（P<0.05）。蛋白质印迹法检测表明,随着TAE226浓度的增加,E-cadherin蛋白的表达增加,Vimentin蛋白的表达降低（P<0.05）。结论 TAE226能有效抑制人口腔鳞状细胞癌细胞株的EMT进程,有望成为治疗口腔鳞状细胞癌的有效药物之一。

关键词: 黏着斑激酶抑制剂TAE226, 上皮间质转化, 口腔鳞状细胞癌

Abstract:

Objective To study the effect of the focal adhesion kinase inhibitor TAE226 on epithelial-mesenchymal transition (EMT) in human oral squamous cell carcinoma (OSCC) cell line. Methods HSC-3 and HSC-4 cells were cultured with TAE226 under different concentrations (0, 1, 5, and 10 μmol·L^-1) for 24, 48, and 72 h. Real-time quantitative polymerase chain reaction was performed to detect the mRNA expressions of E-cadherin and Vimentin. The protein expressions of E-cadherin and Vimentin were determined by Western blot assay after 48 h of TAE226 treatment. Results Real-time quantitative polymerase chain reaction showed that increasing the TAE226 dose and reaction time resulted in increased and decreased E-cadherin and Vimentin mRNA expressions, respectively (P<0.05). Western blot assays showed that increasing the TAE226 dose resulted in increased and decreased E-cadherin and Vimentin protein expressions, respectively (P<0.05). Conclusion TAE226, which is expected to be an effective drug for OSCC treatment, can effectively inhibit the EMT of the OSCC cell line.

Key words: focal adhesion kinase inhibitor TAE226, epithelial-mesenchymal transition, oral squamous cell carcinoma

中图分类号:

R739.8

邹湘渝,曾琴,刘萍,聂敏海. 黏着斑激酶抑制剂TAE226对人口腔鳞状细胞癌细胞上皮间质转化的影响[J]. 华西口腔医学杂志, 2020, 38(1): 17-22.

Zou Xiangyu,Zeng Qin,Liu Ping,Nie Minhai. Effect of the focal adhesion kinase inhibitor TAE226 on the epithelial-mesenchymal transition in human oral squamous cell carcinoma cell line[J]. West China Journal of Stomatology, 2020, 38(1): 17-22.

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参考文献 22

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引物名称	引物序列（5’- 3’）	片段大小/bp
GAPDH	F- GACAGTCAGCCGCATCTTCT	395
	R- AAATGAGCCCCAGCCTTCTC
E-cadherin	F- GAGTGCCAACTGGACCATTCAGTA	106
	R- AGTCACCCACCTCTAAGGCCATC
Vimentin	F- CTCTCAAAGATGCCCAGGAG	176
	R- GCACGATCCAACTCTTCCTC

TAE226浓度/	作用时间
μmol·L^-1	24 h	48 h	72 h
0	1.00±0.25^aA	1.01±0.10^aA	0.80±0.13^bA
1	1.98±0.66^aB	3.77±0.34^bB	6.41±0.16^cB
5	4.15±0.02^aC	8.08±0.12^bC	14.52±0.97^cC
10	10.10±0.84^aD	11.18±0.09^bD	20.58±0.32^cD

TAE226浓度/	作用时间
μmol·L^-1	24 h	48 h	72 h
0	1.01±0.18^aA	1.61±0.12^bA	3.16±0.97^cA
1	0.90±0.13^aB	0.75±0.06^bB	0.55±0.21^cB
5	0.76±0.44^aC	0.51±0.03^bC	0.15±0.29^cC
10	0.25±0.18^aD	0.11±0.05^bD	0.08±0.00^cD

TAE226浓度/	作用时间
μmol·L^-1	24 h	48 h	72 h
0	1.01±0.18^aA	0.71±0.09^bA	0.68±0.08^cA
1	1.40±0.10^aB	3.20±0.36^bB	10.01±0.29^cB
5	2.15±0.50^aC	11.42±1.22^bC	23.65±1.07^cC
10	3.70±0.34^aD	16.68±0.53^bD	27.08±1.02^cD

TAE226浓度/	作用时间
μmol·L^-1	24 h	48 h	72 h
0	0.98±0.10^aA	1.87±0.38^bA	3.22±0.65^cA
1	0.97±0.32^aA	0.73±0.68^bB	0.45±0.17^cB
5	0.75±0.27^aB	0.41±0.07^bC	0.20±0.09^cC
10	0.45±0.21^aC	0.07±0.20^bD	0.04±0.01^cD

黏着斑激酶抑制剂TAE226对人口腔鳞状细胞癌细胞上皮间质转化的影响

Effect of the focal adhesion kinase inhibitor TAE226 on the epithelial-mesenchymal transition in human oral squamous cell carcinoma cell line

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TAE226浓度/μmol·L^-1	E-cadherin	Vimentin
0	0.63±0.15	1.54±0.02
1	0.95±0.04	1.12±0.12
5	1.13±0.02	0.79±0.19
10	1.28±0.10	0.57±0.15

TAE226浓度/μmol·L^-1	E-cadherin	Vimentin
0	0.57±0.12	1.39±0.12
1	0.95±0.04	1.13±0.03
5	1.17±0.47	0.96±0.04
10	1.34±0.06	0.51±0.27

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