华西口腔医学杂志 ›› 2019, Vol. 37 ›› Issue (3): 304-308.doi: 10.7518/hxkq.2019.03.015

• 临床研究 • 上一篇    下一篇

涎腺腺样囊性癌患者口腔微生物多样性研究

刘兴,杨其芬,甘宁,杨德琴()   

  1. 重庆医科大学附属口腔医院种植科 口腔疾病与生物医学重庆市重点实验室 重庆市高校市级口腔生物医学工程重点实验室,重庆 401147
  • 收稿日期:2018-11-19 修回日期:2019-03-05 出版日期:2019-06-01 发布日期:2019-06-12
  • 通讯作者: 杨德琴 E-mail:yangdeqin@hospital.cqmu.edu.cn
  • 作者简介:刘兴,住院医师,硕士,E-mail:2362585980@qq.com
  • 基金资助:
    国家自然科学基金面上项目(31571508);国家自然科学基金面上项目(31371473);渝中区科委重点项目(2012);重庆市渝北区科委课题[2017(农社)45]

Oral microbiological diversity in patients with salivary adenoid cystic carcinoma

Xing Liu,Qifen Yang,Ning Gan,Deqin Yang()   

  1. Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China
  • Received:2018-11-19 Revised:2019-03-05 Online:2019-06-01 Published:2019-06-12
  • Contact: Deqin Yang E-mail:yangdeqin@hospital.cqmu.edu.cn
  • Supported by:
    The National Natural Science Foundation of China(31571508);The National Natural Science Foundation of China(31371473);The Key Project of Yuzhong District Science and Technology Commission(2012);The Science and Technology Project of Yubei District Chongqing [grant No. 2017 (agriculture society) 45]

摘要:

目的 探讨涎腺腺样囊性癌(SACC)患者口腔微生物的多样性和群落差异。方法 采集13例SACC患者与10例健康人的唾液样本,提取其口腔菌群的总DNA,经通用引物扩增16s rRNA基因,利用高通量测序平台进行细菌16S rRNA基因(V3-V4)区高通量测序分析后,利用Mothur软件分析微生物的多样性和群落结构。结果 SACC组优势种群有16种(相对丰度>1%),依次是:链球菌属(36.68%),奈瑟菌属(8.55%),普雷沃菌属_7(7.53%),韦荣球菌属(6.37%)等。对照组优势种群有15种,依次有:链球菌属(18.41%),奈瑟菌属(18.20%),普雷沃菌属_7(8.89%),卟啉单胞菌属(6.20%),梭杆菌属(5.86%),韦荣球菌属(5.82%)等。2组间有统计学差异的菌门包括厚壁菌门、变形菌门、梭杆菌门(P<0.05)。有统计学差异的菌属包括链球菌属、奈瑟菌属和卟啉单胞菌属(P<0.05),而嗜二氧化碳噬细胞菌属仅在SACC患者中检出。结论 SACC患者的唾液微生物与健康人群唾液微生物的种群结构存在明显差异。

关键词: 涎腺腺样囊性癌, 高通量测序, 唾液, 微生物多样性

Abstract:

Objective The aim of this study was to identify the differences in microbial diversity and community in patients with salivary adenoid cystic carcinoma (SACC). Methods Saliva was collected from 13 patients with SACC confirmed by histopathological diagnosis and 10 healthy control subjects. Total metagenomic DNA was extracted. The DNA amplicons of the V3-V4 hypervariable regions of the 16S rRNA gene were generated and subjected to high-throughput sequencing. Microbial diversity and community structure were analyzed with Mothur software. Results A total of 16 genera of dominant bacteria in the SACC group were found, including Streptococcus (36.68%), Neisseria (8.55%), Prevotella_7 (7.53%), and Veillonella (6.37%), whereas 15 dominant bacteria in the control group were found, including Streptococcus (18.41%), Neisseria (18.20%), Prevotella-7 (8.89%), Porphyromonas (6.20%), Fusobacterium (5.86%) and Veillonella (5.82%). The statistically different phyla between the two groups were Firmicutes, Proteobacteria and Fusobacterium (P<0.05). The statistically different genera between the two groups were Streptococcus, Neisseria andPorphyromonas (P<0.05), and Capnocytophaga was only detected in patients with SACC. Conclusion Significant differences were observed in the oral microorganisms between the two groups.

Key words: salivary adenoid cystic carcinoma, high-throughput sequencing, saliva, microbial diversity

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