与重症低龄儿童龋病相关的唾液微生物群落研究

doi:10.7518/hxkq.2018.02.007

华西口腔医学杂志 ›› 2018, Vol. 36 ›› Issue (2): 150-155.doi: 10.7518/hxkq.2018.02.007

与重症低龄儿童龋病相关的唾液微生物群落研究

孙同正¹, 滕飞^2,³, 贾松菠¹, 唐永平¹, 姜明¹, 黄适², 袁晓¹, 李晓岚³, 杨芳^1,³

1.青岛大学附属青岛市市立医院口腔医学中心,青岛 266071
2.中国科学院青岛生物能源与过程研究所,青岛 266100
3.中山大学广东省口腔医学重点实验室,广州 510055

收稿日期:2017-10-05 修回日期:2017-12-22 出版日期:2018-04-10 发布日期:2018-04-10
作者简介:
孙同正,硕士,E-mail：1576437971@qq.com
基金资助:
国家自然科学基金（81670979, 31600099）;青岛市自助创新计划（应用基础研究项目-青年专项）（16-5-1-67-jch）;广东省口腔医学重点实验室开放课题（KF2016120101）

Salivary microbial communities associated with severe early childhood caries

Tongzheng Sun¹, Fei Teng^2,³, Songbo Jia¹, Yongping Tang¹, Ming Jiang¹, Shi Huang², Xiao Yuan¹, Xiaolan Li³, Fang Yang^1,³

1. Stomatology Center, Qingdao Muni-cipal Hospital, The Affiliatel Hospital of Qingdao University, Qingdao 266071, China
2. Qingdao Biological Energy Process Research Institute of Chinese Academy of Sciences, Qingdao 266100, China
3. Guangdong Provincial Key Laboratory of Oral Diseases, Sun Yat-sen University, Guangzhou 510055, China

Received:2017-10-05 Revised:2017-12-22 Online:2018-04-10 Published:2018-04-10
Supported by:
Supported by: The National Natural Science Foundation of China (81670979, 31600099);The Self-help Innovation Plan of Qingdao (Application of Basic Research Project-Youth Special) (16-5-1-67-jch);Open Fund of Guangdong Provincial Key Laboratory of Oral Diseases (KF2016120101). Correspondence: Yang Fang, E-mail: fancy-yf@163.com.

摘要/Abstract

摘要：

目的通过高通量测序技术研究重症低龄儿童龋病和健康者唾液的菌群结构及其差异。方法在青岛市崂山区儿童中,经口腔检查选取健康（H组）和重症低龄龋病（C组）儿童各24名,采取唾液样本,提取其DNA进行聚合酶链式反应扩增,利用454测序平台对16S rRNA V1—V3区进行双端测序,对细菌群落结构及多样性进行差异分析。结果 C组唾液菌群物种丰度高于H组（P<0.05）,两组唾液菌群结构的差异有统计学意义（P<0.01）,且C组的群落结构更为相似和保守（P<0.001）;鉴别出C组高表达的可疑致龋微生物（P<0.1）及H组高表达的健康相关微生物（P<0.1）;基于唾液菌属图谱建立的龋病风险评估模型区分健康和龋病者的准确率可高达70%以上。结论唾液菌群和特定细菌种类,如比例升高的Prevotella菌属有助于评估和筛选低龄儿童龋病风险。

关键词: 低龄儿童龋, 口腔菌群, 唾液, 元基因组学

Abstract:

Objective To compare the salivary microbial profiles of healthy subjects and those with severe early childhood caries (S-ECC) by using high-throughput sequencing. Methods Salivary samples were obtained from children with S-ECC (group C, n=24) and healthy children (group H,n=24). Total metagenomic DNA was extracted, and DNA amplicons of the V1-V3 hypervariable region of the 16S rRNA gene were generated and subjected to 454 sequencing. The characteristics of oral microbial communities from the two groups were compared based on microbial diversity and taxonomy assignment. Results First, the microbial richness was significantly higher in group C than group H (P<0.05). Second, the microbial com-munity structure was significantly different for the groups H and C (P<0.01). In addition, caries microbiota was significantly conserved in group C (P<0.001). High expression of suspected cariogenic microorganisms in group C (P<0.1) and health related microorganisms in group H (P<0.1) were identified. Finally, models of caries risk assessment were proposed to distin-guish caries from healthy subjects with over 70% accuracy.Conclusion Salivary microbiota and certain taxa, such as caries-associated taxa (Prevotella), may be useful to screen/assess the children’s risk of developing caries.

Key words: early childhood caries, oral microbiota, saliva, metagenomic

中图分类号:

R788

孙同正, 滕飞, 贾松菠, 唐永平, 姜明, 黄适, 袁晓, 李晓岚, 杨芳. 与重症低龄儿童龋病相关的唾液微生物群落研究[J]. 华西口腔医学杂志, 2018, 36(2): 150-155.

Tongzheng Sun, Fei Teng, Songbo Jia, Yongping Tang, Ming Jiang, Shi Huang, Xiao Yuan, Xiaolan Li, Fang Yang. Salivary microbial communities associated with severe early childhood caries[J]. West China Journal of Stomatology, 2018, 36(2): 150-155.

图/表 5

参考文献 18

[1]	Anderson M.Risk assessment and epidemiology of dental caries: review of the literature[J]. Pediatr Dent, 2002, 24(5):377-385.
[2]	Hallett KB, O’Rourke PK. Pattern and severity of early child-hood caries[J]. Community Dent Oral Epidemiol, 2006, 34(1):25-35.
[3]	Alm A, Wendt LK, Koch G, et al.Prevalence of approximal caries in posterior teeth in 15-year-old Swedish teenagers in relation to their caries experience at 3 years of age[J]. Caries Res, 2007, 41(5):392-398.
[4]	Rassool GH.WHO releases new report on global problem of oral diseases[J]. J Adv Nurs, 2004, 47(3):344.
[5]	Oral health: prevention is key[J]. Lancet, 2009, 373(9657):1.
[6]	Yang F, Zeng X, Ning K, et al.Saliva microbiomes distin-guish caries-active from healthy human populations[J]. ISME J, 2012, 6(1):1-10.
[7]	Marsh PD. Dental diseases—are these examples of ecolo-gical catastrophes[J]. Int J Dent Hyg, 2006, 4(Suppl 1):3-10; discussion 50-52.
[8]	Turnbaugh PJ, Ley RE, Hamady M, et al.The human micro-biome project[J]. Nature, 2007, 449(7164):804-810.
[9]	Teng F, Yang F, Huang S, et al.Prediction of early childhood caries via spatial-temporal variations of oral microbiota[J]. Cell Host Microbe, 2015, 18(3):296-306.
[10]	Ling Z, Kong J, Jia P, et al.Analysis of oral microbiota in children with dental caries by PCR-DGGE and barcoded pyrosequencing[J]. Microb Ecol, 2010, 60(3):677-690.
[11]	Jiang S, Gao X, Jin L, et al.Salivary microbiome diversity in caries-free and caries-affected children[J]. Int J Mol Sci, 2016, 17(12):E1978.
[12]	Ling Z, Liu X, Wang Y, et al.Pyrosequencing analysis of the salivary microbiota of healthy Chinese children and adults[J]. Microb Ecol, 2013, 65(2):487-495.
[13]	Crielaard W, Zaura E, Schuller AA, et al.Exploring the oral microbiota of children at various developmental stages of their dentition in the relation to their oral health[J]. BMC Med Genomics, 2011, 4(1):22.
[14]	Takahashi N, Nyvad B.The role of bacteria in the caries process: ecological perspectives[J]. J Dent Res, 2011, 90(3):294-303.
[15]	Gross EL, Beall CJ, Kutsch SR, et al.Beyond Streptococcus mutans: dental caries onset linked to multiple species by 16S rRNA community analysis[J]. PLoS One, 2012, 7(10):e47722.
[16]	Johansson I, Witkowska E, Kaveh B, et al.The microbiome in populations with a low and high prevalence of caries[J]. J Dent Res, 2016, 95(1):80-86.
[17]	Chen L, Qin B, Du M, et al.Extensive description and com-parison of human supra-gingival microbiome in root caries and health[J]. PLoS One, 2015, 10(2):e0117064.
[18]	Simón-Soro A, Belda-Ferre P, Cabrera-Rubio R, et al.A tissue-dependent hypothesis of dental caries[J]. Caries Res, 2013, 47(6):591-600.

与重症低龄儿童龋病相关的唾液微生物群落研究

Salivary microbial communities associated with severe early childhood caries

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