含纳米抗菌无机填料树脂粘接剂的合成及相关性能研究

doi:10.7518/hxkq.2018.01.009

华西口腔医学杂志 ›› 2018, Vol. 36 ›› Issue (1): 46-51.doi: 10.7518/hxkq.2018.01.009

含纳米抗菌无机填料树脂粘接剂的合成及相关性能研究

陈德英¹(), 胡格¹, 周传健², 葛建华², 吴峻岭¹()

1.山东大学口腔医学院口腔修复学教研所,山东省口腔组织再生重点实验室,济南 250012
2.山东大学材料科学与工程学院高分子材料研究所,济南 250061

收稿日期:2017-07-01 修回日期:2017-09-28 出版日期:2018-02-07 发布日期:2018-02-01
作者简介:
陈德英,硕士,E-mail:atd172@outlook.com
基金资助:
[基金项目] 国家自然科学基金面上项目（81671032）;山东省自然科学基金面上项目（ZR2014HM073）

Antibacterial dental adhesive containing nanoantibacterial inorganic fillers

Deying Chen¹(), Ge Hu¹, Chuanjian Zhou², Jianhua Ge², Junling Wu¹()

1.1. Dept. of Prosthodontics, School of Stomatology, Shandong University, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250012, China
2. Research Institute of Polymer Materials, School of Materials Science and Engineering, Shandong University, Jinan 250061, China

Received:2017-07-01 Revised:2017-09-28 Online:2018-02-07 Published:2018-02-01
Supported by:
Supported by: The National Natural Science Foundation of China (81671032);Shandong Provincial Natural Science Founda-tion (ZR2014HM073).

摘要/Abstract

摘要：

目的合成含纳米抗菌无机填料的新型树脂粘接剂,并探讨其粘接强度、力学性能、体外抗菌性能等。方法制备长链烷基季铵盐修饰的纳米抗菌二氧化硅填料,并以0%、2.5%、5.0%、7.5%及10%的质量分数添加到树脂粘接剂中,生成新型纳米抗菌树脂粘接剂。以0%组作为对照组,用牙本质剪切试验来测试粘接强度;用三点弯曲试验来测试力学性能;建立人牙菌斑生物膜体外模型,通过菌落计数、乳酸代谢分析及活/死细菌染色等手段来评价新型树脂粘接剂对牙菌斑生物膜的影响。结果加入5.0%的纳米抗菌无机填料时,树脂粘接剂的牙本质粘接强度为28.9 MPa,弯曲强度及弹性模量分别为86.6 MPa和4.2 GPa,与对照组比较,粘接强度及力学性能明显提高（P<0.05）；当抗菌无机填料含量达到2.5%时，新型抗菌树脂粘接剂对牙菌斑生物膜代谢产生明显抑制作用，显示了良好的抗菌性能（P<0.05）。结论含一定质量分数（5.0%）新型纳米抗菌无机填料的树脂粘接剂具有较强的粘接强度、力学性能及抗菌功能，为进一步临床应用打下基础。

关键词: 季铵盐, 无机填料, 树脂粘接剂, 牙菌斑生物膜, 抗菌性

Abstract:

Objective This investigation aimed to develop a novel antibacterial dental adhesive containing nanoantibacterial inorganic fillers and measure the dentin bonding strength, mechanical properties, and antibacterial property of the novel adhesive in vitro.Methods Novel nanoantibacterial inorganic fillers containing quaternary ammonium salt with long chain alkyl were synthesized on the basis of previous research. These novel nanoantibacterial inorganic fillers were added into the dental adhesive to prepare novel nanoantibacterial dental resin composite at mass fractions of 0%, 2.5%, 5.0%, 7.5%, and 10%; 0% was used as control. Dentin shear bonding test was used to evaluate the bonding strength. Flexural test was utilized to measure the novel resin composite flexural strength and elastic modulus. A dental plaque microcosm biofilm model with human saliva as inoculum was formed. Colony forming unit, lactic acid production, and live/dead assay of the biofilm on novel dental adhesive were calculated to assess the effect of novel dental adhesive on human dental plaque microcosm biofilm. Results The dentin shear bond strength, flexural strength, and elastic modulus were 28.9 MPa, 86.6 MPa, and 4.2 GPa, respectively, when the nanoantibacterial inorganic filler mass fraction in the dental adhesive reached approximately 5.0%. Consequently, the dentin shear bond strength and mechanical properties signi-ficantly increased. Addition of 2.5% nanoantibacterial inor-ganic fillers into the dental adhesive exerted no adverse effect on the mechanical properties significantly (P>0.05). Dental adhesive containing 5% or more nanoantibacterial inorganic fillers inhibited the metabolic activity of the dental plaque micro-cosm biofilm significantly, thereby displaying a strong antibacterial potency (P<0.05).Conclusion This novel antibacterial dental adhesive, which contained 5.0% nanoantibacterial inorganic filler, exhibited promising bonding strength, mechanical property, and antibacterial ability. Hence, this adhesive can be potentially used in caries inhibition in dental application.

Key words: quaternary ammonium salt, inorganic fillers, dental adhesive, dental plaque microcosm biofilm, anti-bacterial property

中图分类号:

R783

陈德英, 胡格, 周传健, 葛建华, 吴峻岭. 含纳米抗菌无机填料树脂粘接剂的合成及相关性能研究[J]. 华西口腔医学杂志, 2018, 36(1): 46-51.

Deying Chen, Ge Hu, Chuanjian Zhou, Jianhua Ge, Junling Wu. Antibacterial dental adhesive containing nanoantibacterial inorganic fillers[J]. West China Journal of Stomatology, 2018, 36(1): 46-51.

图/表 3

表 1

表 2

图 1

参考文献 16

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组别	填料质量	粘接强	弯曲强	弹性模
组别	分数/%	度/MPa	度/MPa	量/GPa
A	0	21.9±3.2^a	64.5±10.0 ^a	3.0±0.5 ^a
B	2.5	25.0±3.2^b	72.0±11.0 ^a	3.4±0.7^a
C	5.0	28.9±3.5^c	86.6±14.4 ^b	4.2±0.8 ^b
D	7.5	17.7±3.4 ^d	66.0±12.0^a,c	2.9±0.5 ^a,c
E	10	16.8±3.4^d	50.6±9.4^d	2.4±0.4^d
F值		18.39	10.32	10.08
P值		<0.01	<0.01	<0.01

组别	填料质量分数/%	菌落计数/CFU			乳酸量/mmol·L^-1
组别	填料质量分数/%	全菌落	总链球菌	变异链球菌	乳酸量/mmol·L^-1
A	0	（5.4±1.0）×10^{9 a}	（5.3±0.9）×10^{8 a}	（5.0±1.0）×10^{7 a}	23.40±4.17^a
B	2.5	（5.0±1.0）×10^{6 b}	（5.5±0.8）×10^{5 b}	（5.4±1.0）×10^{3 b}	0.23±0.04 ^b
C	5.0	（5.7±1.0）×10^{6 b}	（5.8±1.0）×10^{5 b}	（5.5±0.9）×10^{3 b}	0.24±0.05 ^b
D	7.5	（5.6±1.2）×10^{6 b}	（5.6±1.0）×10^{5 b}	（5.9±1.1）×10^{3 b}	0.23±0.04 ^b
E	10	（5.9±1.1） ×10^{6 b}	（5.3±1.0）×10^{5 b}	（5.0±1.1）×10^{3 b}	0.25±0.05 ^b
F值		167.9	194.3	157.9	185.0
P值		<0.01	<0.01	<0.01	<0.01

含纳米抗菌无机填料树脂粘接剂的合成及相关性能研究

Antibacterial dental adhesive containing nanoantibacterial inorganic fillers

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